Gene description for DAP3
Gene name death associated protein 3
Gene symbol DAP3
Other names/aliases DAP-3
MRP-S29
MRPS29
bMRP-10
Species Homo sapiens
 Database cross references - DAP3
ExoCarta ExoCarta_7818
Vesiclepedia VP_7818
Entrez Gene 7818
HGNC 2673
MIM 602074
UniProt P51398  
 DAP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DAP3
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mitochondrial translational elongation GO:0070125 TAS
    mitochondrial translational initiation GO:0070124 TAS
    mitochondrial translation GO:0032543 TAS
    organelle organization GO:0006996 TAS
    mitochondrial translational termination GO:0070126 TAS
    apoptotic signaling pathway GO:0097190 TAS
    apoptotic mitochondrial changes GO:0008637 IEA
Subcellular Localization
    mitochondrial ribosome GO:0005761 IEA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrion GO:0005739 IDA
    small ribosomal subunit GO:0015935 IEA
    nucleolus GO:0005730 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified DAP3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNBP 7555
Affinity Capture-MS Homo sapiens
2 ESR1  
Reconstituted Complex Homo sapiens
3 THRA  
Reconstituted Complex Homo sapiens
4 AR 367
Reconstituted Complex Homo sapiens
5 FADD 8772
Invivo Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 ESR2  
Reconstituted Complex Homo sapiens
7 NR3C1 2908
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
8 TNFRSF25  
Reconstituted Complex Homo sapiens
9 PPARA  
Reconstituted Complex Homo sapiens
10 AHR 196
Reconstituted Complex Homo sapiens
11 DAP3 7818
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 TNFRSF10A 8797
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
14 PGR  
Reconstituted Complex Homo sapiens
15 HIF1A 3091
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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