ExoCarta: Gene summary

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Gene description for MRPS5
Gene name	mitochondrial ribosomal protein S5
Gene symbol	MRPS5
Other names/aliases	MRP-S5 S5mt
Species	Homo sapiens
Database cross references - MRPS5
ExoCarta	ExoCarta_64969
Vesiclepedia	VP_64969
Entrez Gene	64969
HGNC	14498
MIM	611972
UniProt	P82675
MRPS5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942
Bone marrow mesenchymal stem cells	36408942

Gene ontology annotations for MRPS5

Molecular Function
	RNA binding	GO:0003723	HDA
	structural constituent of ribosome	GO:0003735	IBA
Biological Process
	translation	GO:0006412	IBA
	mitochondrial translation	GO:0032543	NAS
Subcellular Localization
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	NAS
	mitochondrial inner membrane	GO:0005743	TAS
	mitochondrial small ribosomal subunit	GO:0005763	IBA
	mitochondrial small ribosomal subunit	GO:0005763	IDA
	mitochondrial small ribosomal subunit	GO:0005763	NAS

Experiment description of studies that identified MRPS5 in sEVs

Experiment ID

489

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 6

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

490

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 7

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

491

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 8

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

492

MISEV standards
✔ EM	Biophysical techniques
✔ Cd9\|Cd81\|Cd63\|Gapdh\|Sdcbp\|Lamp1\|Aqp1\|Rab5a\|Icam1\|Cd82\|Itga2b\|Tsg101\|Lamp2\|Rab35\|Flot1\|Flot2\|Cd151\|Rab5b\|Tfrc\|Uchl1	Enriched markers
✔ Canx	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

36408942

Organism

Rattus norvegicus

Experiment description

Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells

Authors

"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."

Journal name

Proteomics

Publication year

2023

Sample

Bone marrow mesenchymal stem cells

Sample name

BMSC - Passage 9

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for MRPS5

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

GNL2

29889

Affinity Capture-MS

Homo sapiens

UBE2H

7328

Affinity Capture-MS

Homo sapiens

ZBTB38

253461

Affinity Capture-MS

Homo sapiens

NUPL2

11097

Affinity Capture-MS

Homo sapiens

MRPS27

23107

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SLIRP

81892

Proximity Label-MS

Homo sapiens

H1FNT

Affinity Capture-MS

Homo sapiens

SNX3

8724

Affinity Capture-MS

Homo sapiens

RBMS3

Affinity Capture-MS

Homo sapiens

RPL36AL

6166

Affinity Capture-MS

Homo sapiens

ZNF324B

Affinity Capture-MS

Homo sapiens

RBMS2

5939

Affinity Capture-MS

Homo sapiens

LHX2

Proximity Label-MS

Homo sapiens

RPL13A

23521

Affinity Capture-MS

Homo sapiens

RPL26

6154

Affinity Capture-MS

Homo sapiens

TRUB2

Proximity Label-MS

Homo sapiens

KLF8

Proximity Label-MS

Homo sapiens

MTG2

Proximity Label-MS

Homo sapiens

NGRN

Proximity Label-MS

Homo sapiens

RPL10

6134

Affinity Capture-MS

Homo sapiens

MRPL24

Co-fractionation

Homo sapiens

CAND1

55832

Affinity Capture-MS

Homo sapiens

HNRNPR

10236

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPUSD4

84881

Proximity Label-MS

Homo sapiens

DNAJC15

Proximity Label-MS

Homo sapiens

MTRF1

Proximity Label-MS

Homo sapiens

GFM2

84340

Proximity Label-MS

Homo sapiens

CCDC90B

Proximity Label-MS

Homo sapiens

LRPPRC

10128

Proximity Label-MS

Homo sapiens

RPL4

6124

Affinity Capture-MS

Homo sapiens

IGF2BP3

10643

Affinity Capture-MS

Homo sapiens

ZBTB2

57621

Affinity Capture-MS

Homo sapiens

MCU

90550

Proximity Label-MS

Homo sapiens

SULF2

55959

Affinity Capture-MS

Homo sapiens

CRYZ

1429

Proximity Label-MS

Homo sapiens

TFAM

7019

Proximity Label-MS

Homo sapiens

PMPCA

23203

Proximity Label-MS

Homo sapiens

SDHB

6390

Co-fractionation

Homo sapiens

ZNF485

Affinity Capture-MS

Homo sapiens

FBXW7

Affinity Capture-MS

Homo sapiens

HINT2

84681

Proximity Label-MS

Homo sapiens

MRPS23

51649

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPS24

64951

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF460

Affinity Capture-MS

Homo sapiens

DDRGK1

65992

Affinity Capture-MS

Homo sapiens

KLF15

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPS9

6203

Affinity Capture-MS

Homo sapiens

MRPS26

64949

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SURF1

Proximity Label-MS

Homo sapiens

ADARB1

104

Affinity Capture-MS

Homo sapiens

EXD2

Proximity Label-MS

Homo sapiens

TULP3

7289

Affinity Capture-MS

Homo sapiens

ERAL1

Affinity Capture-MS

Homo sapiens

ZC3HAV1

56829

Affinity Capture-MS

Homo sapiens

KLF4

Affinity Capture-MS

Homo sapiens

E4F1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZNF689

Affinity Capture-MS

Homo sapiens

MRPS7

51081

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TLX3

Proximity Label-MS

Homo sapiens

PMPCB

9512

Proximity Label-MS

Homo sapiens

RBM42

Affinity Capture-MS

Homo sapiens

FASTKD2

Proximity Label-MS

Homo sapiens

MTIF2

4528

Proximity Label-MS

Homo sapiens

RPL19

6143

Affinity Capture-MS

Homo sapiens

MTERF3

Proximity Label-MS

Homo sapiens

MZT1

Affinity Capture-MS

Homo sapiens

MDH2

4191

Proximity Label-MS

Homo sapiens

SRSF6

6431

Affinity Capture-MS

Homo sapiens

KLF16

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ACTA2

Affinity Capture-MS

Homo sapiens

MRPS25

64432

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZKSCAN8

Affinity Capture-MS

Homo sapiens

C6orf203

Proximity Label-MS

Homo sapiens

COX15

1355

Proximity Label-MS

Homo sapiens

FASTKD5

Proximity Label-MS

Homo sapiens

SRP14

6727

Affinity Capture-MS

Homo sapiens

PARK2

Affinity Capture-MS

Homo sapiens

MRPS34

65993

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZBTB47

Affinity Capture-MS

Homo sapiens

PTCD3

55037

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TRA2A

29896

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TMOD1

Affinity Capture-MS

Homo sapiens

TUFM

7284

Co-fractionation		Homo sapiens
Proximity Label-MS		Homo sapiens

ZBTB48

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TMEM43

79188

Co-fractionation

Homo sapiens

HSCB

150274

Proximity Label-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

RPL35

11224

Affinity Capture-MS

Homo sapiens

SRSF7

6432

Affinity Capture-MS

Homo sapiens

DES

1674

Affinity Capture-MS

Homo sapiens

MRPS18B

28973

Affinity Capture-MS

Homo sapiens

NTRK1

4914

Affinity Capture-MS

Homo sapiens

PRR4

11272

Affinity Capture-MS

Homo sapiens

MRPS6

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CCDC109B

55013

Proximity Label-MS

Homo sapiens

MOV10

4343

Affinity Capture-RNA

Homo sapiens

ZMPSTE24

10269

Co-fractionation

Homo sapiens

FBXL6

Affinity Capture-MS

Homo sapiens

LCN1

3933

Affinity Capture-MS

Homo sapiens

100

RPS6

6194

Affinity Capture-MS

Homo sapiens

101

MTFMT

Proximity Label-MS

Homo sapiens

102

MRPL11

65003

Proximity Label-MS

Homo sapiens

103

MTRF1L

Proximity Label-MS

Homo sapiens

104

TMEM70

Proximity Label-MS

Homo sapiens

105

MRPS33

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

106

GLTSCR2

Affinity Capture-MS

Homo sapiens

107

MRPL2

51069

Affinity Capture-MS

Homo sapiens

108

CHCHD1

Proximity Label-MS

Homo sapiens

109

RPF1

Affinity Capture-MS

Homo sapiens

110

CCDC59

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

111

TLX2

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

112

OTC

5009

Proximity Label-MS

Homo sapiens

113

MRRF

Proximity Label-MS

Homo sapiens

114

ABT1

29777

Affinity Capture-MS

Homo sapiens

115

MRPS12

Proximity Label-MS

Homo sapiens

116

AURKAIP1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

117

DAP3

7818

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

118

DNAJC4

Proximity Label-MS

Homo sapiens

119

MRPS35

60488

Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens

120

RBM47

54502

Affinity Capture-MS

Homo sapiens

121

YBX1

4904

Affinity Capture-MS

Homo sapiens

122

MRPS11

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

123

MTIF3

Proximity Label-MS

Homo sapiens

124

SRSF4

6429

Affinity Capture-MS

Homo sapiens

125

EEF2

1938

Co-fractionation

Homo sapiens

126

SRSF1

6426

Affinity Capture-MS

Homo sapiens

127

USP36

Affinity Capture-MS

Homo sapiens

128

CUL3

8452

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

129

RPUSD3

Proximity Label-MS

Homo sapiens

130

RMND1

55005

Proximity Label-MS

Homo sapiens

131

FAHD1

81889

Affinity Capture-MS

Homo sapiens

132

HNRNPA1

3178

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

133

DHX30

22907

Proximity Label-MS

Homo sapiens

134

CORO1C

23603

Co-fractionation

Homo sapiens

135

NXF1

10482

Affinity Capture-RNA

Homo sapiens

136

SOX15

Affinity Capture-MS

Homo sapiens

137

C12orf65

Proximity Label-MS

Homo sapiens

138

MYCN

Affinity Capture-MS

Homo sapiens

139

PRR3

Affinity Capture-MS

Homo sapiens

140

CBX6

Affinity Capture-MS

Homo sapiens

141

TACO1

Proximity Label-MS

Homo sapiens

142

MTG1

Proximity Label-MS

Homo sapiens

143

MYC

Affinity Capture-MS

Homo sapiens

144

NOL12

79159

Affinity Capture-MS

Homo sapiens

145

TSFM

10102

Proximity Label-MS

Homo sapiens

146

ACAD9

28976

Proximity Label-MS

Homo sapiens

147

RPL18

6141

Affinity Capture-MS

Homo sapiens

148

VWA8

23078

Proximity Label-MS

Homo sapiens

149

CLPB

81570

Proximity Label-MS

Homo sapiens

150

GALNT2

2590

Co-fractionation

Homo sapiens

151

MRPS17

51373

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

152

METTL15

Proximity Label-MS

Homo sapiens

153

ZC3H3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

154

UQCRFS1P1

Co-fractionation

Homo sapiens

155

MCUR1

63933

Proximity Label-MS

Homo sapiens

156

FLOT1

10211

Co-fractionation

Homo sapiens

157

ZNF263

Affinity Capture-MS

Homo sapiens

158

SOX2

Proximity Label-MS

Homo sapiens

159

ZNF574

Affinity Capture-MS

Homo sapiens

160

RPL13

6137

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

161

CLPP

8192

Proximity Label-MS

Homo sapiens

162

1431

Proximity Label-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

163

ZNF707

Affinity Capture-MS

Homo sapiens

164

PLEKHA4

57664

Affinity Capture-MS

Homo sapiens

165

RIT1

6016

Negative Genetic

Homo sapiens

166

RC3H1

149041

Affinity Capture-MS

Homo sapiens

167

AUH

549

Proximity Label-MS

Homo sapiens

168

MRPS2

51116

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

169

PDGFB

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

170

ILF3

3609

Co-fractionation

Homo sapiens

171

ZFC3H1

196441

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

172

DNAJC19

131118

Proximity Label-MS

Homo sapiens

173

MCAT

27349

Affinity Capture-MS

Homo sapiens

174

TP53

7157

Affinity Capture-MS

Homo sapiens

175

RPL6

6128

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

176

ICT1

3396

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

177

APOBEC3D

Affinity Capture-MS

Homo sapiens

178

LONP1

9361

Proximity Label-MS

Homo sapiens

179

MRPS18C

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

180

MRPS31

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

181

PDHA1

5160

Proximity Label-MS

Homo sapiens

182

DNAJA3

9093

Proximity Label-MS

Homo sapiens

183

SMURF1

57154

Affinity Capture-MS

Homo sapiens

184

SQSTM1

8878

Proximity Label-MS

Homo sapiens

185

MRPS15

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

186

COX8A

Proximity Label-MS

Homo sapiens

187

MRPS10

55173

Co-fractionation

Homo sapiens

188

ZNF764

92595

Affinity Capture-MS

Homo sapiens

189

ZNF467

Affinity Capture-MS

Homo sapiens

190

ZNF331

Affinity Capture-MS

Homo sapiens

191

C21orf33

Proximity Label-MS

Homo sapiens

192

MRPL18

29074

Affinity Capture-MS

Homo sapiens

193

SSBP1

6742

Proximity Label-MS

Homo sapiens

194

KLF12

Proximity Label-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

195

UFL1

23376

Affinity Capture-MS

Homo sapiens

196

FASTKD3

Proximity Label-MS

Homo sapiens

197

NEIL1

Affinity Capture-MS

Homo sapiens

198

ACTA1

Affinity Capture-MS

Homo sapiens

199

ZNF556

80032

Affinity Capture-MS

Homo sapiens

200

SRSF3

6428

Affinity Capture-MS

Homo sapiens

201

GFM1

85476

Proximity Label-MS

Homo sapiens

202

AARS2

Proximity Label-MS

Homo sapiens

203

TEFM

Proximity Label-MS

Homo sapiens

204

MRPS9

64965

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

205

RPL23

9349

Co-fractionation

Homo sapiens

206

SLC25A51

Proximity Label-MS

Homo sapiens

207

RPS8

6202

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

208

TBRG4

9238

Proximity Label-MS

Homo sapiens

209

TRMT10B

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

210

GRSF1

2926

Proximity Label-MS

Homo sapiens

211

C1QBP

708

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

212

GLI4

Affinity Capture-MS

Homo sapiens

213

KRAS

3845

Negative Genetic

Homo sapiens

214

HNRNPU

3192

Affinity Capture-MS

Homo sapiens

215

C17orf80

55028

Proximity Label-MS

Homo sapiens

216

METTL17

Proximity Label-MS

Homo sapiens

217

MRPS22

56945

Affinity Capture-MS

Homo sapiens

218

C8orf82

Proximity Label-MS

Homo sapiens

219

C19orf52

Proximity Label-MS

Homo sapiens

220

LUC7L

55692

Affinity Capture-MS

Homo sapiens

221

DDX56

Affinity Capture-MS

Homo sapiens

222

DDX28

Proximity Label-MS

Homo sapiens

223

RPL17

6139

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

View the network

Pathways in which MRPS5 is involved

Pathway	Evidence	Source
Metabolism of proteins	TAS	Reactome
Metabolism of proteins	IEA	Reactome
Mitochondrial translation	TAS	Reactome
Mitochondrial translation	IEA	Reactome
Mitochondrial translation elongation	TAS	Reactome
Mitochondrial translation elongation	IEA	Reactome
Mitochondrial translation initiation	IEA	Reactome
Mitochondrial translation termination	TAS	Reactome
Translation	TAS	Reactome
Translation	IEA	Reactome

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