Gene description for CCDC109B
Gene name coiled-coil domain containing 109B
Gene symbol CCDC109B
Other names/aliases MCUb
Species Homo sapiens
 Database cross references - CCDC109B
ExoCarta ExoCarta_55013
Vesiclepedia VP_55013
Entrez Gene 55013
HGNC 26076
UniProt Q9NWR8  
 CCDC109B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CCDC109B
Molecular Function
    monoatomic ion channel activity GO:0005216 ISS
    protein binding GO:0005515 IPI
    calcium channel inhibitor activity GO:0019855 IBA
    calcium channel inhibitor activity GO:0019855 IDA
    calcium channel inhibitor activity GO:0019855 ISS
Biological Process
    mitochondrial calcium ion transmembrane transport GO:0006851 ISS
    calcium import into the mitochondrion GO:0036444 IBA
    calcium import into the mitochondrion GO:0036444 NAS
    regulation of macrophage activation GO:0043030 ISS
    skeletal muscle tissue regeneration GO:0043403 ISS
    mitochondrial calcium ion homeostasis GO:0051560 IBA
    mitochondrial calcium ion homeostasis GO:0051560 ISS
    mitochondrial calcium ion homeostasis GO:0051560 NAS
    negative regulation of calcium import into the mitochondrion GO:0110099 IDA
    positive regulation of skeletal muscle satellite cell differentiation GO:1902726 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISS
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    membrane GO:0016020 ISS
    calcium channel complex GO:0034704 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    uniplex complex GO:1990246 IBA
    uniplex complex GO:1990246 IDA
    uniplex complex GO:1990246 NAS
 Experiment description of studies that identified CCDC109B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CCDC109B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 MRPS27 23107
Proximity Label-MS Homo sapiens
3 AFG3L2 10939
Proximity Label-MS Homo sapiens
4 SLIRP 81892
Proximity Label-MS Homo sapiens
5 MRPL48  
Proximity Label-MS Homo sapiens
6 NDUFS1 4719
Proximity Label-MS Homo sapiens
7 PTPMT1 114971
Proximity Label-MS Homo sapiens
8 MRPL24  
Proximity Label-MS Homo sapiens
9 MRPL37 51253
Proximity Label-MS Homo sapiens
10 MRPL27 51264
Proximity Label-MS Homo sapiens
11 CNP 1267
Proximity Label-MS Homo sapiens
12 NDUFS7 374291
Proximity Label-MS Homo sapiens
13 MRPL50 54534
Proximity Label-MS Homo sapiens
14 GATB  
Proximity Label-MS Homo sapiens
15 PDHB 5162
Proximity Label-MS Homo sapiens
16 MRPL49 740
Proximity Label-MS Homo sapiens
17 PDE12 201626
Proximity Label-MS Homo sapiens
18 SETDB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NDUFS2 4720
Proximity Label-MS Homo sapiens
20 CARS2 79587
Proximity Label-MS Homo sapiens
21 LRPPRC 10128
Proximity Label-MS Homo sapiens
22 MRPS5 64969
Proximity Label-MS Homo sapiens
23 NDUFS3 4722
Proximity Label-MS Homo sapiens
24 MRPL20 55052
Proximity Label-MS Homo sapiens
25 MCU 90550
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
26 MRPS34 65993
Proximity Label-MS Homo sapiens
27 OGDH 4967
Proximity Label-MS Homo sapiens
28 IARS2 55699
Proximity Label-MS Homo sapiens
29 NDUFA7 4701
Proximity Label-MS Homo sapiens
30 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 HINT2 84681
Proximity Label-MS Homo sapiens
32 MRPS23 51649
Proximity Label-MS Homo sapiens
33 MRPS24 64951
Proximity Label-MS Homo sapiens
34 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
35 ETFA 2108
Proximity Label-MS Homo sapiens
36 MRPS26 64949
Proximity Label-MS Homo sapiens
37 ERAL1  
Proximity Label-MS Homo sapiens
38 MRPL39 54148
Proximity Label-MS Homo sapiens
39 NDUFV1 4723
Proximity Label-MS Homo sapiens
40 PHB 5245
Proximity Label-MS Homo sapiens
41 NDUFAB1 4706
Proximity Label-MS Homo sapiens
42 ATP5H 10476
Proximity Label-MS Homo sapiens
43 SLC30A9 10463
Proximity Label-MS Homo sapiens
44 BCS1L 617
Proximity Label-MS Homo sapiens
45 MRPL4 51073
Proximity Label-MS Homo sapiens
46 NME4 4833
Proximity Label-MS Homo sapiens
47 FAF2 23197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MTIF2 4528
Proximity Label-MS Homo sapiens
49 MRPL47 57129
Proximity Label-MS Homo sapiens
50 TRMT10C 54931
Proximity Label-MS Homo sapiens
51 MDH2 4191
Proximity Label-MS Homo sapiens
52 STOML2 30968
Proximity Label-MS Homo sapiens
53 MRPS30 10884
Proximity Label-MS Homo sapiens
54 MRPL13  
Proximity Label-MS Homo sapiens
55 ATAD3B 83858
Proximity Label-MS Homo sapiens
56 MRPS25 64432
Proximity Label-MS Homo sapiens
57 MRPS33  
Proximity Label-MS Homo sapiens
58 NDUFS4 4724
Proximity Label-MS Homo sapiens
59 MRPL45 84311
Proximity Label-MS Homo sapiens
60 ZBTB16  
Two-hybrid Homo sapiens
61 ACOT2 10965
Proximity Label-MS Homo sapiens
62 NT5DC2 64943
Proximity Label-MS Homo sapiens
63 ATPAF1  
Proximity Label-MS Homo sapiens
64 PAM16  
Proximity Label-MS Homo sapiens
65 PTCD3 55037
Proximity Label-MS Homo sapiens
66 NDUFS8 4728
Proximity Label-MS Homo sapiens
67 MMAB 326625
Proximity Label-MS Homo sapiens
68 IL2RA  
Affinity Capture-MS Homo sapiens
69 MRPS6  
Proximity Label-MS Homo sapiens
70 PDHX 8050
Proximity Label-MS Homo sapiens
71 MRPL3  
Proximity Label-MS Homo sapiens
72 DBT 1629
Proximity Label-MS Homo sapiens
73 NDUFAF7  
Proximity Label-MS Homo sapiens
74 NDUFAF3 25915
Proximity Label-MS Homo sapiens
75 ATP5B 506
Proximity Label-MS Homo sapiens
76 ABCB7 22
Proximity Label-MS Homo sapiens
77 COX4I1 1327
Proximity Label-MS Homo sapiens
78 MRPL41 64975
Proximity Label-MS Homo sapiens
79 OXA1L  
Proximity Label-MS Homo sapiens
80 MRPL44  
Proximity Label-MS Homo sapiens
81 ACAT1 38
Proximity Label-MS Homo sapiens
82 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MRPL21 219927
Proximity Label-MS Homo sapiens
84 HADHA 3030
Proximity Label-MS Homo sapiens
85 DAP3 7818
Proximity Label-MS Homo sapiens
86 NDUFB10 4716
Proximity Label-MS Homo sapiens
87 MRPS35 60488
Proximity Label-MS Homo sapiens
88 NDUFA9 4704
Proximity Label-MS Homo sapiens
89 NDUFV2 4729
Proximity Label-MS Homo sapiens
90 LETM1 3954
Proximity Label-MS Homo sapiens
91 SECTM1 6398
Affinity Capture-MS Homo sapiens
92 RTN4IP1  
Proximity Label-MS Homo sapiens
93 MRPS7 51081
Proximity Label-MS Homo sapiens
94 ETFB 2109
Proximity Label-MS Homo sapiens
95 MRPL9 65005
Proximity Label-MS Homo sapiens
96 MRPS14  
Proximity Label-MS Homo sapiens
97 NFS1 9054
Proximity Label-MS Homo sapiens
98 ATP5F1 515
Proximity Label-MS Homo sapiens
99 GLS 2744
Proximity Label-MS Homo sapiens
100 PYCR1 5831
Proximity Label-MS Homo sapiens
101 ELAC2 60528
Proximity Label-MS Homo sapiens
102 GADD45GIP1  
Proximity Label-MS Homo sapiens
103 VARS2  
Proximity Label-MS Homo sapiens
104 PYCR2 29920
Proximity Label-MS Homo sapiens
105 ECHS1 1892
Proximity Label-MS Homo sapiens
106 GLUD1 2746
Proximity Label-MS Homo sapiens
107 MRPL15 29088
Proximity Label-MS Homo sapiens
108 TACO1  
Proximity Label-MS Homo sapiens
109 NEFL 4747
Proximity Label-MS Homo sapiens
110 DLST 1743
Proximity Label-MS Homo sapiens
111 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 NDUFA6  
Proximity Label-MS Homo sapiens
113 ACAD9 28976
Proximity Label-MS Homo sapiens
114 MRPS36 92259
Proximity Label-MS Homo sapiens
115 VWA8 23078
Proximity Label-MS Homo sapiens
116 CLPB 81570
Proximity Label-MS Homo sapiens
117 COX2 4513
Proximity Label-MS Homo sapiens
118 MRPS17 51373
Proximity Label-MS Homo sapiens
119 MRPL38  
Proximity Label-MS Homo sapiens
120 DLAT 1737
Proximity Label-MS Homo sapiens
121 MRPL18 29074
Proximity Label-MS Homo sapiens
122 PPIF 10105
Proximity Label-MS Homo sapiens
123 IMMT 10989
Proximity Label-MS Homo sapiens
124 AK4 205
Proximity Label-MS Homo sapiens
125 CS 1431
Proximity Label-MS Homo sapiens
126 MRPL42  
Proximity Label-MS Homo sapiens
127 C17orf80 55028
Proximity Label-MS Homo sapiens
128 PHB2 11331
Proximity Label-MS Homo sapiens
129 NDUFAF2  
Proximity Label-MS Homo sapiens
130 ATP5J 522
Proximity Label-MS Homo sapiens
131 MRPS2 51116
Proximity Label-MS Homo sapiens
132 TXNDC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ICT1 3396
Proximity Label-MS Homo sapiens
134 MRPS28  
Proximity Label-MS Homo sapiens
135 PNPLA8 50640
Proximity Label-MS Homo sapiens
136 LARS2 23395
Proximity Label-MS Homo sapiens
137 MRPS18C  
Proximity Label-MS Homo sapiens
138 MRPL53  
Proximity Label-MS Homo sapiens
139 MRPL43 84545
Proximity Label-MS Homo sapiens
140 COX5A 9377
Proximity Label-MS Homo sapiens
141 NDUFA12 55967
Proximity Label-MS Homo sapiens
142 GRPEL1 80273
Proximity Label-MS Homo sapiens
143 NDUFV3 4731
Proximity Label-MS Homo sapiens
144 MRPS10 55173
Proximity Label-MS Homo sapiens
145 SUCLA2 8803
Proximity Label-MS Homo sapiens
146 SHMT2 6472
Proximity Label-MS Homo sapiens
147 ATAD3A 55210
Proximity Label-MS Homo sapiens
148 ME2 4200
Proximity Label-MS Homo sapiens
149 NNT 23530
Proximity Label-MS Homo sapiens
150 NIPSNAP1 8508
Proximity Label-MS Homo sapiens
151 NDUFA5 4698
Proximity Label-MS Homo sapiens
152 MRPS18B 28973
Proximity Label-MS Homo sapiens
153 NDUFA2 4695
Proximity Label-MS Homo sapiens
154 SEC62 7095
Affinity Capture-MS Homo sapiens
155 ACOT1 641371
Proximity Label-MS Homo sapiens
156 MRPL46  
Proximity Label-MS Homo sapiens
157 FECH 2235
Proximity Label-MS Homo sapiens
158 MRPS16  
Proximity Label-MS Homo sapiens
159 GRSF1 2926
Proximity Label-MS Homo sapiens
160 NDUFAF4 29078
Proximity Label-MS Homo sapiens
161 ATP5C1 509
Proximity Label-MS Homo sapiens
162 MRPS31  
Proximity Label-MS Homo sapiens
163 MRPS22 56945
Proximity Label-MS Homo sapiens
164 MRPL28 10573
Proximity Label-MS Homo sapiens
165 NDUFS6  
Proximity Label-MS Homo sapiens
166 MRPS9 64965
Proximity Label-MS Homo sapiens
167 TIMM44 10469
Proximity Label-MS Homo sapiens
168 ECSIT 51295
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CCDC109B is involved
PathwayEvidenceSource
Mitochondrial calcium ion transport TAS Reactome
Processing of SMDT1 TAS Reactome
Transport of small molecules TAS Reactome





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