Gene description for GLS
Gene name glutaminase
Gene symbol GLS
Other names/aliases AAD20
GAC
GAM
GLS1
KGA
Species Homo sapiens
 Database cross references - GLS
ExoCarta ExoCarta_2744
Entrez Gene 2744
HGNC 4331
MIM 138280
UniProt O94925  
 GLS identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GLS
Molecular Function
    glutaminase activity GO:0004359 NAS
    protein binding GO:0005515 IPI
Biological Process
    synaptic transmission GO:0007268 TAS
    protein homotetramerization GO:0051289 IDA
    cellular amino acid biosynthetic process GO:0008652 TAS
    suckling behavior GO:0001967 IEA
    glutamine catabolic process GO:0006543 NAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    neurotransmitter secretion GO:0007269 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    regulation of respiratory gaseous exchange by neurological system process GO:0002087 IEA
    small molecule metabolic process GO:0044281 TAS
    glutamate biosynthetic process GO:0006537 TAS
    glutamate secretion GO:0014047 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    mitochondrion GO:0005739 NAS
    cytosol GO:0005829 IEA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified GLS in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GLS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNTA1 6640
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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