Gene description for SHC2
Gene name SHC (Src homology 2 domain containing) transforming protein 2
Gene symbol SHC2
Other names/aliases SCK
SHCB
SLI
Species Homo sapiens
 Database cross references - SHC2
ExoCarta ExoCarta_25759
Vesiclepedia VP_25759
Entrez Gene 25759
HGNC 29869
MIM 605217
UniProt P98077  
 SHC2 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
 Gene ontology annotations for SHC2
Molecular Function
    protein binding GO:0005515 IPI
    receptor tyrosine kinase binding GO:0030971 IBA
Biological Process
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    intracellular signal transduction GO:0035556 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
 Experiment description of studies that identified SHC2 in sEVs
1
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SHC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA8 143471
Affinity Capture-MS Homo sapiens
2 GLS 2744
Affinity Capture-MS Homo sapiens
3 ARHGAP32  
Affinity Capture-Western Homo sapiens
4 ERBB2 2064
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
5 VWA8 23078
Affinity Capture-MS Homo sapiens
6 BCKDK 10295
Affinity Capture-MS Homo sapiens
7 GRB2 2885
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 SDR39U1  
Affinity Capture-MS Homo sapiens
10 MTIF2 4528
Affinity Capture-MS Homo sapiens
11 RSAD1  
Affinity Capture-MS Homo sapiens
12 KIT 3815
Reconstituted Complex Homo sapiens
13 MTG2  
Affinity Capture-MS Homo sapiens
14 ERBB4 2066
Reconstituted Complex Homo sapiens
15 EGFR 1956
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 GATB  
Affinity Capture-MS Homo sapiens
17 ERBB3 2065
Reconstituted Complex Homo sapiens
18 TMLHE 55217
Affinity Capture-MS Homo sapiens
19 AP2M1 1173
Affinity Capture-MS Homo sapiens
20 FLT1 2321
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 AARS2  
Affinity Capture-MS Homo sapiens
22 PC 5091
Affinity Capture-MS Homo sapiens
23 ERRFI1 54206
Affinity Capture-MS Homo sapiens
24 AP2A1 160
Affinity Capture-MS Homo sapiens
25 TARS2 80222
Affinity Capture-MS Homo sapiens
26 AP1B1 162
Affinity Capture-MS Homo sapiens
27 MET 4233
Reconstituted Complex Homo sapiens
28 CYP24A1  
Affinity Capture-MS Homo sapiens
29 KIAA0391  
Affinity Capture-MS Homo sapiens
30 ADCK4 79934
Affinity Capture-MS Homo sapiens
31 VAV2 7410
Affinity Capture-MS Homo sapiens
32 KDR  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
33 METTL15  
Affinity Capture-MS Homo sapiens
34 PTCD1 26024
Affinity Capture-MS Homo sapiens
35 SARDH 1757
Affinity Capture-MS Homo sapiens
36 DARS2 55157
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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