Gene description for FLT1
Gene name fms-related tyrosine kinase 1
Gene symbol FLT1
Other names/aliases FLT
FLT-1
VEGFR-1
VEGFR1
Species Homo sapiens
 Database cross references - FLT1
ExoCarta ExoCarta_2321
Vesiclepedia VP_2321
Entrez Gene 2321
HGNC 3763
MIM 165070
UniProt P17948  
 FLT1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FLT1
Molecular Function
    VEGF-A-activated receptor activity GO:0036326 IDA
    vascular endothelial growth factor-activated receptor activity GO:0005021 IMP
    growth factor binding GO:0019838 IPI
    placental growth factor-activated receptor activity GO:0036332 IDA
    identical protein binding GO:0042802 IEA
    protein binding GO:0005515 IPI
    transmembrane receptor protein tyrosine kinase activity GO:0004714 TAS
    VEGF-B-activated receptor activity GO:0036327 IDA
    ATP binding GO:0005524 IEA
Biological Process
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    vascular endothelial growth factor signaling pathway GO:0038084 TAS
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    sprouting angiogenesis GO:0002040 IEA
    cell migration GO:0016477 IMP
    embryonic morphogenesis GO:0048598 ISS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    protein autophosphorylation GO:0046777 IDA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IMP
    patterning of blood vessels GO:0001569 IEA
    positive regulation of angiogenesis GO:0045766 IMP
    cell differentiation GO:0030154 IEA
    positive regulation of MAP kinase activity GO:0043406 IDA
    vascular endothelial growth factor receptor-1 signaling pathway GO:0036323 IDA
    positive regulation of cell proliferation GO:0008284 TAS
    positive regulation of phospholipase C activity GO:0010863 IMP
    positive regulation of phosphatidylinositol 3-kinase signaling GO:0014068 IMP
    monocyte chemotaxis GO:0002548 IDA
    positive regulation of MAPK cascade GO:0043410 IDA
    positive regulation of cell migration GO:0030335 IDA
    blood vessel morphogenesis GO:0048514 ISS
Subcellular Localization
    focal adhesion GO:0005925 IDA
    plasma membrane GO:0005886 TAS
    receptor complex GO:0043235 IDA
    integral component of plasma membrane GO:0005887 IDA
    endosome GO:0005768 IEA
    extracellular space GO:0005615 TAS
 Experiment description of studies that identified FLT1 in exosomes
1
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FLT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 KDR  
Invivo Homo sapiens
Biochemical Activity Homo sapiens
3 PLCG2 5336
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 SHC1 6464
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 PGF  
Reconstituted Complex Homo sapiens
6 VEGFB  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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