Gene description for HCK
Gene name HCK proto-oncogene, Src family tyrosine kinase
Gene symbol HCK
Other names/aliases JTK9
p59Hck
p61Hck
Species Homo sapiens
 Database cross references - HCK
ExoCarta ExoCarta_3055
Vesiclepedia VP_3055
Entrez Gene 3055
HGNC 4840
MIM 142370
UniProt P08631  
 HCK identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for HCK
Molecular Function
    phosphotyrosine residue binding GO:0001784 IPI
    protein tyrosine kinase activity GO:0004713 EXP
    protein tyrosine kinase activity GO:0004713 IMP
    protein tyrosine kinase activity GO:0004713 TAS
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    lipid binding GO:0008289 EXP
    histone H3Y41 kinase activity GO:0035401 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    leukocyte migration involved in immune response GO:0002522 TAS
    innate immune response-activating signaling pathway GO:0002758 TAS
    negative regulation of inflammatory response to antigenic stimulus GO:0002862 TAS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 TAS
    inflammatory response GO:0006954 IEA
    cell adhesion GO:0007155 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    integrin-mediated signaling pathway GO:0007229 TAS
    mesoderm development GO:0007498 TAS
    positive regulation of cell population proliferation GO:0008284 IMP
    regulation of cell shape GO:0008360 IMP
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
    cell differentiation GO:0030154 IBA
    positive regulation of actin filament polymerization GO:0030838 TAS
    lipopolysaccharide-mediated signaling pathway GO:0031663 TAS
    regulation of actin cytoskeleton organization GO:0032956 IMP
    intracellular signal transduction GO:0035556 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    leukocyte degranulation GO:0043299 TAS
    respiratory burst after phagocytosis GO:0045728 TAS
    protein autophosphorylation GO:0046777 IMP
    regulation of inflammatory response GO:0050727 TAS
    regulation of phagocytosis GO:0050764 IMP
    regulation of DNA-binding transcription factor activity GO:0051090 IMP
    type II interferon-mediated signaling pathway GO:0060333 TAS
    regulation of podosome assembly GO:0071801 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    lysosome GO:0005764 IDA
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    caveola GO:0005901 IDA
    focal adhesion GO:0005925 IMP
    cytoplasmic side of plasma membrane GO:0009898 IMP
    transport vesicle GO:0030133 IEA
    cell projection GO:0042995 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified HCK in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HCK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BPIFA1 51297
Two-hybrid Homo sapiens
2 ABL2  
Two-hybrid Homo sapiens
3 CCDC88B 283234
Two-hybrid Homo sapiens
4 RBPMS 11030
Two-hybrid Homo sapiens
5 ABL1 25
Affinity Capture-Western Homo sapiens
6 PAK2 5062
Reconstituted Complex Homo sapiens
7 RPL10 6134
Reconstituted Complex Homo sapiens
8 FLT3  
Affinity Capture-Western Homo sapiens
9 SPRR2A  
Reconstituted Complex Homo sapiens
10 APP 351
Reconstituted Complex Homo sapiens
11 IL6ST 3572
Affinity Capture-Western Homo sapiens
12 PLEKHG4  
Two-hybrid Homo sapiens
13 KRT40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 SRC 6714
Affinity Capture-Western Homo sapiens
15 MAP4K5 11183
Two-hybrid Homo sapiens
16 KHDRBS1 10657
Protein-peptide Homo sapiens
FRET Homo sapiens
Affinity Capture-Western Homo sapiens
17 MTUS2 23281
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 PLCG1 5335
Co-localization Homo sapiens
19 GOPC 57120
Two-hybrid Homo sapiens
20 KPRP 448834
Two-hybrid Homo sapiens
21 Mbp 17196
Protein-peptide Mus musculus
22 FCGR1A  
Affinity Capture-Western Homo sapiens
23 ERBB2 2064
Reconstituted Complex Homo sapiens
24 KIF14 9928
Affinity Capture-MS Homo sapiens
25 WIPF1 7456
Reconstituted Complex Homo sapiens
26 BCAR1 9564
Reconstituted Complex Homo sapiens
27 KIT 3815
Reconstituted Complex Homo sapiens
28 FASLG 356
Protein-peptide Homo sapiens
29 FHL5 9457
Two-hybrid Homo sapiens
30 CCR3  
Affinity Capture-Western Homo sapiens
31 RUFY1 80230
Affinity Capture-MS Homo sapiens
32 CYB5D2  
Two-hybrid Homo sapiens
33 ERBB3 2065
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
34 SKAP2 8935
Affinity Capture-Western Homo sapiens
35 CBL 867
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 SH2B2  
Two-hybrid Homo sapiens
37 FLT1 2321
Co-localization Homo sapiens
38 PIK3CB 5291
Co-localization Homo sapiens
Co-localization Homo sapiens
39 CYSRT1 375791
Two-hybrid Homo sapiens
40 CRKL 1399
Co-localization Homo sapiens
41 CHST15 51363
Affinity Capture-Western Homo sapiens
42 AGR2 10551
Two-hybrid Homo sapiens
43 UNC119 9094
Reconstituted Complex Homo sapiens
44 ACTB 60
Affinity Capture-MS Homo sapiens
45 BTK 695
Reconstituted Complex Homo sapiens
46 TOX2  
Two-hybrid Homo sapiens
47 RAPGEF1 2889
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
48 PECAM1 5175
Affinity Capture-Western Homo sapiens
49 KRTAP10-7  
Two-hybrid Homo sapiens
50 CTAG1A  
Two-hybrid Homo sapiens
51 ANLN 54443
Affinity Capture-MS Homo sapiens
52 NOTCH2NL 388677
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
53 PRRG4  
Reconstituted Complex Homo sapiens
54 ZSCAN4  
Two-hybrid Homo sapiens
55 KRTAP1-3  
Two-hybrid Homo sapiens
56 ELMO1 9844
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
57 KRT35 3886
Two-hybrid Homo sapiens
58 KRTAP10-8  
Two-hybrid Homo sapiens
59 TEKT4  
Two-hybrid Homo sapiens
60 ERBB4 2066
Reconstituted Complex Homo sapiens
61 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Co-localization Homo sapiens
62 GAB1  
Reconstituted Complex Homo sapiens
63 CSF3R  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
64 MYC  
Dosage Lethality Homo sapiens
65 TRIM27  
Two-hybrid Homo sapiens
66 MET 4233
Reconstituted Complex Homo sapiens
67 TRPV4 59341
Affinity Capture-Western Homo sapiens
68 ENTHD2  
Two-hybrid Homo sapiens
69 WNT2  
Two-hybrid Homo sapiens
70 FHL2 2274
Two-hybrid Homo sapiens
71 MDM2  
Protein-peptide Homo sapiens
72 RASA1 5921
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
73 KRTAP3-1  
Two-hybrid Homo sapiens
74 TNK2 10188
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
75 KRT36 8689
Two-hybrid Homo sapiens
76 MDK 4192
Two-hybrid Homo sapiens
77 CTAG1B  
Two-hybrid Homo sapiens
78 INCA1  
Two-hybrid Homo sapiens
79 CDC37 11140
Affinity Capture-Western Homo sapiens
80 NBPF19  
Two-hybrid Homo sapiens
81 MKRN3  
Two-hybrid Homo sapiens
82 KRTAP1-1  
Two-hybrid Homo sapiens
83 RASA3 22821
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
84 CREBBP  
Reconstituted Complex Homo sapiens
85 PNMA1 9240
Two-hybrid Homo sapiens
86 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
87 ADAM15 8751
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
88 WAS 7454
Reconstituted Complex Homo sapiens
89 DOK1 1796
Reconstituted Complex Homo sapiens
90 SOS1 6654
Co-localization Homo sapiens
91 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
92 AR 367
Reconstituted Complex Homo sapiens
93 HSF2BP  
Two-hybrid Homo sapiens
94 KRT34  
Two-hybrid Homo sapiens
95 C1orf74  
Affinity Capture-MS Homo sapiens
96 BCR 613
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
97 MED28  
Affinity Capture-Western Homo sapiens
98 KRTAP10-9  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which HCK is involved
PathwayEvidenceSource
Anti-inflammatory response favouring Leishmania parasite infection TAS Reactome
Cytokine Signaling in Immune system IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
FCGR activation TAS Reactome
FCGR3A-mediated IL10 synthesis TAS Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FLT3 Signaling IEA Reactome
FLT3 signaling through SRC family kinases IEA Reactome
HIV Infection TAS Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Inactivation of CSF3 (G-CSF) signaling IEA Reactome
Inactivation of CSF3 (G-CSF) signaling TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Leishmania infection TAS Reactome
Leishmania parasite growth and survival TAS Reactome
Leishmania phagocytosis TAS Reactome
Nef and signal transduction TAS Reactome
Parasite infection TAS Reactome
Parasitic Infection Pathways TAS Reactome
Regulation of signaling by CBL IEA Reactome
Regulation of signaling by CBL TAS Reactome
Signaling by CSF1 (M-CSF) in myeloid cells IEA Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by CSF3 (G-CSF) TAS Reactome
Signaling by Interleukins IEA Reactome
Signaling by Interleukins TAS Reactome
The role of Nef in HIV-1 replication and disease pathogenesis TAS Reactome
Viral Infection Pathways TAS Reactome





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