Gene description for HCK
Gene name HCK proto-oncogene, Src family tyrosine kinase
Gene symbol HCK
Other names/aliases JTK9
p59Hck
p61Hck
Species Homo sapiens
 Database cross references - HCK
ExoCarta ExoCarta_3055
Vesiclepedia VP_3055
Entrez Gene 3055
HGNC 4840
MIM 142370
UniProt P08631  
 HCK identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for HCK
Molecular Function
    protein tyrosine kinase activity GO:0004713 IMP
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IBA
    protein binding GO:0005515 IPI
    receptor binding GO:0005102 IBA
    ATP binding GO:0005524 IEA
Biological Process
    cytokine-mediated signaling pathway GO:0019221 TAS
    inflammatory response GO:0006954 IEA
    viral process GO:0016032 TAS
    positive regulation of actin cytoskeleton reorganization GO:2000251 IMP
    innate immune response GO:0045087 TAS
    integrin-mediated signaling pathway GO:0007229 TAS
    innate immune response-activating signal transduction GO:0002758 TAS
    regulation of sequence-specific DNA binding transcription factor activity GO:0051090 IMP
    regulation of inflammatory response GO:0050727 TAS
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    leukocyte degranulation GO:0043299 TAS
    positive regulation of actin filament polymerization GO:0030838 TAS
    regulation of defense response to virus by virus GO:0050690 TAS
    protein phosphorylation GO:0006468 TAS
    regulation of cell shape GO:0008360 IMP
    cell adhesion GO:0007155 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    regulation of podosome assembly GO:0071801 IDA
    lipopolysaccharide-mediated signaling pathway GO:0031663 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IMP
    regulation of phagocytosis GO:0050764 IMP
    negative regulation of apoptotic process GO:0043066 IMP
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    cell differentiation GO:0030154 IBA
    protein autophosphorylation GO:0046777 IMP
    cellular response to peptide hormone stimulus GO:0071375 IBA
    mesoderm development GO:0007498 TAS
    leukocyte migration involved in immune response GO:0002522 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    respiratory burst after phagocytosis GO:0045728 TAS
    positive regulation of cell proliferation GO:0008284 IMP
Subcellular Localization
    caveola GO:0005901 IDA
    actin filament GO:0005884 IDA
    nucleus GO:0005634 IEA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IMP
    lysosome GO:0005764 IDA
    cytosol GO:0005829 TAS
    cell projection GO:0042995 IEA
    Golgi apparatus GO:0005794 IEA
    focal adhesion GO:0005925 IMP
    transport vesicle GO:0030133 IEA
 Experiment description of studies that identified HCK in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 191
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HCK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 FCGR1A  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 ELMO1 9844
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 WIPF1 7456
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 PLAUR 5329
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 ABL1 25
Affinity Capture-Western Homo sapiens
7 CCR3  
Affinity Capture-Western Homo sapiens
8 RASA3 22821
Invitro Homo sapiens
Invivo Homo sapiens
9 FCGR2A 2212
Affinity Capture-MS Homo sapiens
10 HCK 3055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ADAM15 8751
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 IL6ST 3572
Invivo Homo sapiens
13 SKAP2 8935
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 CBL 867
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
15 DOK1 1796
Reconstituted Complex Homo sapiens
16 CSF3R  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 CHST15 51363
Invivo Homo sapiens
18 WAS 7454
Reconstituted Complex Homo sapiens
19 UNC119 9094
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 BTK 695
Reconstituted Complex Homo sapiens
21 TRPV4 59341
Invitro Homo sapiens
Invivo Homo sapiens
22 RAPGEF1 2889
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 BCAR1 9564
Reconstituted Complex Homo sapiens
24 PECAM1 5175
Affinity Capture-Western Homo sapiens
25 BCR 613
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which HCK is involved
PathwayEvidenceSource
FCGR activation TAS Reactome
Nef and signal transduction TAS Reactome
Regulation of signaling by CBL TAS Reactome





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