Gene description for DOK1
Gene name docking protein 1, 62kDa (downstream of tyrosine kinase 1)
Gene symbol DOK1
Other names/aliases P62DOK
Species Homo sapiens
 Database cross references - DOK1
ExoCarta ExoCarta_1796
Vesiclepedia VP_1796
Entrez Gene 1796
HGNC 2990
MIM 602919
UniProt Q99704  
 DOK1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DOK1
Molecular Function
    insulin receptor binding GO:0005158 IEA
    protein binding GO:0005515 IPI
    receptor signaling protein activity GO:0005057 IEA
Biological Process
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    Ras protein signal transduction GO:0007265 IEA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified DOK1 in exosomes
1
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
2 RASA1 5921
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 KIT 3815
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 TEC 7006
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
5 SRC 6714
Reconstituted Complex Homo sapiens
6 FGR 2268
Reconstituted Complex Homo sapiens
7 DOK2 9046
Affinity Capture-Western Homo sapiens
8 DOK1 1796
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 HCK 3055
Reconstituted Complex Homo sapiens
10 CRKL 1399
Reconstituted Complex Homo sapiens
11 NCK2 8440
Reconstituted Complex Homo sapiens
12 YES1 7525
Reconstituted Complex Homo sapiens
13 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
14 ABL1 25
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 PLCG1 5335
Reconstituted Complex Homo sapiens
16 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 PIK3R1 5295
Reconstituted Complex Homo sapiens
18 RET 5979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 PTPN6 5777
Invivo Homo sapiens
20 PTPRH 5794
Invivo Homo sapiens
21 SH2D1A 4068
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DOK1 is involved
PathwayEvidenceSource
IRS activation TAS Reactome
PI3K Cascade TAS Reactome
PI3K Cascade TAS Reactome
Signal attenuation TAS Reactome





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