Gene description for DOK2
Gene name docking protein 2, 56kDa
Gene symbol DOK2
Other names/aliases p56DOK
p56dok-2
Species Homo sapiens
 Database cross references - DOK2
ExoCarta ExoCarta_9046
Vesiclepedia VP_9046
Entrez Gene 9046
HGNC 2991
MIM 604997
UniProt O60496  
 DOK2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for DOK2
Molecular Function
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IEA
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    Ras protein signal transduction GO:0007265 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DOK2 in exosomes
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RASA1 5921
Affinity Capture-Western Homo sapiens
2 HVCN1 84329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 POT1  
Two-hybrid Homo sapiens
4 SPOP  
Affinity Capture-MS Homo sapiens
5 NCK1 4690
Affinity Capture-Western Homo sapiens
6 CFLAR  
Affinity Capture-MS Homo sapiens
7 SHC1 6464
Affinity Capture-Western Homo sapiens
8 DVL2 1856
Affinity Capture-MS Homo sapiens
9 ADCK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ASF1B  
Affinity Capture-MS Homo sapiens
11 PALM 5064
Affinity Capture-MS Homo sapiens
12 MAPK1 5594
Affinity Capture-MS Homo sapiens
13 RAB10 10890
Affinity Capture-MS Homo sapiens
14 SSSCA1 10534
Affinity Capture-MS Homo sapiens
15 EGFR 1956
Reconstituted Complex Homo sapiens
16 PLEKHO1 51177
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PSMD10 5716
Affinity Capture-MS Homo sapiens
18 IQCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 FAM120B 84498
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TEK  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 DOK1 1796
Affinity Capture-Western Homo sapiens
23 RSBN1  
Affinity Capture-MS Homo sapiens
24 KIF1BP 26128
Two-hybrid Homo sapiens
25 CENPM  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 NCEH1 57552
Affinity Capture-MS Homo sapiens
27 DNPEP 23549
Affinity Capture-MS Homo sapiens
28 YES1 7525
Two-hybrid Homo sapiens
29 EVI2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TMEM184B  
Affinity Capture-MS Homo sapiens
31 FN3K 64122
Affinity Capture-MS Homo sapiens
32 HSPA4L 22824
Affinity Capture-MS Homo sapiens
33 FAM188A 80013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 TMEM38A 79041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 PLCG1 5335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 RET 5979
Affinity Capture-Western Homo sapiens
38 LANCL2 55915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DNAJB11 51726
Affinity Capture-MS Homo sapiens
40 PDK4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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