Gene description for DOK2
Gene name docking protein 2, 56kDa
Gene symbol DOK2
Other names/aliases p56DOK
p56dok-2
Species Homo sapiens
 Database cross references - DOK2
ExoCarta ExoCarta_9046
Entrez Gene 9046
HGNC 2991
MIM 604997
UniProt O60496  
 DOK2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for DOK2
Molecular Function
    receptor signaling protein activity GO:0005057 IEA
    insulin receptor binding GO:0005158 IEA
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 IEA
Biological Process
    blood coagulation GO:0007596 TAS
    cell surface receptor signaling pathway GO:0007166 TAS
    leukocyte migration GO:0050900 TAS
    Ras protein signal transduction GO:0007265 IEA
    signal transduction GO:0007165 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DOK2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DOK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB10 10890
Affinity Capture-MS Homo sapiens
2 RASA1 5921
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 TEK  
Affinity Capture-Western Homo sapiens
4 KIF1BP 26128
Two-hybrid Homo sapiens
5 DOK1 1796
Affinity Capture-Western Homo sapiens
6 HSPA4L 22824
Affinity Capture-MS Homo sapiens
7 LCK 3932
Affinity Capture-MS Homo sapiens
8 SPOP  
Affinity Capture-MS Homo sapiens
9 INPP5D 3635
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 NCK1 4690
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
11 PSMD10 5716
Affinity Capture-MS Homo sapiens
12 CFLAR  
Affinity Capture-MS Homo sapiens
13 EGFR 1956
Reconstituted Complex Homo sapiens
14 SHC1 6464
Affinity Capture-Western Homo sapiens
15 RET 5979
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
16 DNAJB11 51726
Affinity Capture-MS Homo sapiens
17 SSSCA1  
Affinity Capture-MS Homo sapiens
18 ASF1B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DOK2 is involved
PathwayEvidenceSource
Tie2 Signaling TAS Reactome





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