Gene description for PLCG1
Gene name phospholipase C, gamma 1
Gene symbol PLCG1
Other names/aliases NCKAP3
PLC-II
PLC1
PLC148
PLCgamma1
Species Homo sapiens
 Database cross references - PLCG1
ExoCarta ExoCarta_5335
Vesiclepedia VP_5335
Entrez Gene 5335
HGNC 9065
MIM 172420
UniProt P19174  
 PLCG1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PLCG1
Molecular Function
    calcium ion binding GO:0005509 IEA
    glutamate receptor binding GO:0035254 IEA
    receptor signaling protein activity GO:0005057 NAS
    protein kinase binding GO:0019901 IPI
    phospholipase C activity GO:0004629 TAS
    receptor tyrosine kinase binding GO:0030971 IEA
    neurotrophin TRKA receptor binding GO:0005168 IPI
    phosphatidylinositol phospholipase C activity GO:0004435 TAS
    protein binding GO:0005515 IPI
Biological Process
    positive regulation of epithelial cell migration GO:0010634 IMP
    calcium-mediated signaling GO:0019722 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    in utero embryonic development GO:0001701 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    inositol phosphate metabolic process GO:0043647 TAS
    small molecule metabolic process GO:0044281 TAS
    axon guidance GO:0007411 TAS
    blood coagulation GO:0007596 TAS
    activation of MAPKK activity GO:0000186 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    cell migration GO:0016477 IMP
    innate immune response GO:0045087 TAS
    activation of phospholipase C activity GO:0007202 TAS
    signal transduction GO:0007165 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    phospholipid catabolic process GO:0009395 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    cytokine-mediated signaling pathway GO:0019221 TAS
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IMP
    positive regulation of angiogenesis GO:0045766 IDA
    viral process GO:0016032 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    leukocyte migration GO:0050900 TAS
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cell-cell junction GO:0005911 IEA
    plasma membrane GO:0005886 TAS
    cell projection GO:0042995 IDA
    cytosol GO:0005829 TAS
    lamellipodium GO:0030027 IDA
    ruffle GO:0001726 IDA
    COP9 signalosome GO:0008180 IDA
 Experiment description of studies that identified PLCG1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PLCG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDGFA 5154
Affinity Capture-Western Homo sapiens
2 BAG3 9531
Invivo Homo sapiens
Invitro Homo sapiens
3 LAT 27040
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
4 PKN2 5586
Reconstituted Complex Homo sapiens
5 RASA1 5921
Affinity Capture-Western Homo sapiens
6 ERBB2 2064
Invivo Homo sapiens
Invivo Homo sapiens
7 FGFR1 2260
Two-hybrid Homo sapiens
8 GNB2L1 10399
Invivo Homo sapiens
Invitro Homo sapiens
9 GRB2 2885
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 EEF1A1 1915
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 RICS  
Affinity Capture-Western Homo sapiens
12 CD22 933
Invitro Homo sapiens
13 PAK1 5058
Reconstituted Complex Homo sapiens
14 PECAM1 5175
Invitro Homo sapiens
15 SNAP91 9892
Invivo Homo sapiens
Invitro Homo sapiens
16 TRPM7 54822
Invitro Homo sapiens
Two-hybrid Homo sapiens
17 MST1R 4486
Invivo Homo sapiens
18 SOS1 6654
Invitro Homo sapiens
19 PDGFRA 5156
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 KIT 3815
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
21 VAV1 7409
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 PTK2 5747
Affinity Capture-Western Homo sapiens
23 SHB 6461
Affinity Capture-Western Homo sapiens
24 PRKD1 5587
Affinity Capture-Western Homo sapiens
25 EGFR 1956
Invivo Homo sapiens
26 TUB  
Invivo Homo sapiens
Invitro Homo sapiens
27 RET 5979
Invivo Homo sapiens
Invitro Homo sapiens
28 PDGFRB 5159
Affinity Capture-Western Homo sapiens
29 LAT2 7462
Affinity Capture-Western Homo sapiens
30 SOCS7  
Invitro Homo sapiens
31 GAB1  
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
32 FGFR2 2263
Invivo Homo sapiens
33 CBL 867
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
34 PITPNA 5306
Invivo Homo sapiens
Invitro Homo sapiens
35 SELE  
Affinity Capture-Western Homo sapiens
36 DOK1 1796
Reconstituted Complex Homo sapiens
37 LIFR 3977
Invivo Homo sapiens
38 DGKZ  
Affinity Capture-Western Homo sapiens
39 NTRK3 4916
Reconstituted Complex Homo sapiens
40 TNK1  
Invitro Homo sapiens
41 GIT1 28964
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 SH3BP2  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
43 PTPRJ 5795
Invitro Homo sapiens
44 NTRK1 4914
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
45 LCP2 3937
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
46 ZAP70 7535
Affinity Capture-Western Homo sapiens
47 SYNCRIP 10492
Reconstituted Complex Homo sapiens
48 CTSS 1520
Invivo Homo sapiens
Invitro Homo sapiens
49 SOS2 6655
Invivo Homo sapiens
Two-hybrid Homo sapiens
50 WAS 7454
Reconstituted Complex Homo sapiens
51 SRC 6714
Affinity Capture-Western Homo sapiens
52 ITK 3702
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
53 KHDRBS1 10657
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 SYK 6850
Affinity Capture-Western Homo sapiens
55 NTRK2 4915
Reconstituted Complex Homo sapiens
56 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 PICALM 8301
Invitro Homo sapiens
58 PLD2 5338
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
59 ALK 238
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
60 IRS2 8660
Invivo Homo sapiens
61 SHC1 6464
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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