Gene description for GIT1
Gene name G protein-coupled receptor kinase interacting ArfGAP 1
Gene symbol GIT1
Other names/aliases -
Species Homo sapiens
 Database cross references - GIT1
ExoCarta ExoCarta_28964
Vesiclepedia VP_28964
Entrez Gene 28964
HGNC 4272
MIM 608434
UniProt Q9Y2X7  
 GIT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GIT1
Molecular Function
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IEA
Biological Process
    ephrin receptor signaling pathway GO:0048013 TAS
    axon guidance GO:0007411 TAS
    regulation of cytokinesis GO:0032465 IDA
    positive regulation of GTPase activity GO:0043547 IEA
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IDA
    aggresome GO:0016235 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified GIT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GIT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
2 GRK5 2869
Invivo Homo sapiens
3 C8orf33 65265
Two-hybrid Homo sapiens
4 PTPRF 5792
Affinity Capture-Western Homo sapiens
5 GRK6 2870
Invivo Homo sapiens
6 PXN  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
7 EIF6 3692
Two-hybrid Homo sapiens
8 PCLO  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 PPFIA2 8499
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
10 GIT2 9815
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
11 HMOX2 3163
Two-hybrid Homo sapiens
12 PAK2 5062
Affinity Capture-MS Homo sapiens
13 WDR33 55339
Two-hybrid Homo sapiens
14 PFDN1 5201
Two-hybrid Homo sapiens
15 PPFIA1 8500
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 ARHGEF7 8874
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 RGS2  
Two-hybrid Homo sapiens
18 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 TGFB1I1 7041
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
20 SDCCAG3  
Two-hybrid Homo sapiens
21 KIF1A 547
Invivo Homo sapiens
22 LAMTOR5 10542
Two-hybrid Homo sapiens
23 PMF1  
Two-hybrid Homo sapiens
24 TXNDC9 10190
Two-hybrid Homo sapiens
25 SRRT 51593
Two-hybrid Homo sapiens
26 TERF1 7013
Two-hybrid Homo sapiens
27 PTPRZ1  
Affinity Capture-Western Homo sapiens
28 GIT1 28964
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
29 MAN2A2 4122
Two-hybrid Homo sapiens
30 PPFIA4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 DDX24 57062
Two-hybrid Homo sapiens
32 PTK2B 2185
Affinity Capture-Western Homo sapiens
33 TRIB3  
Two-hybrid Homo sapiens
34 GRIP2 80852
Affinity Capture-Western Homo sapiens
35 ARHGEF6 9459
Affinity Capture-Western Homo sapiens
36 PLCG1 5335
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
37 HSPC065  
Two-hybrid Homo sapiens
38 ERC2 26059
Affinity Capture-Western Homo sapiens
39 ADRBK2  
Invivo Homo sapiens
40 PPFIA3 8541
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GIT1 is involved
PathwayEvidenceSource
Ephrin signaling TAS Reactome





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