Gene description for GIT1
Gene name G protein-coupled receptor kinase interacting ArfGAP 1
Gene symbol GIT1
Other names/aliases -
Species Homo sapiens
 Database cross references - GIT1
ExoCarta ExoCarta_28964
Entrez Gene 28964
HGNC 4272
MIM 608434
UniProt Q9Y2X7  
 GIT1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GIT1
Molecular Function
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    GTPase activator activity GO:0005096 IEA
Biological Process
    ephrin receptor signaling pathway GO:0048013 TAS
    axon guidance GO:0007411 TAS
    regulation of cytokinesis GO:0032465 IDA
    positive regulation of GTPase activity GO:0043547 IEA
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IDA
    aggresome GO:0016235 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified GIT1 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GIT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
2 TGFB1I1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 GRK5 2869
Invivo Homo sapiens
4 C8orf33 65265
Two-hybrid Homo sapiens
5 HBXIP  
Two-hybrid Homo sapiens
6 PTPRF 5792
Affinity Capture-Western Homo sapiens
7 GRK6 2870
Invivo Homo sapiens
8 PXN  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
9 TXNDC9  
Two-hybrid Homo sapiens
10 EIF6 3692
Two-hybrid Homo sapiens
11 PFDN1  
Two-hybrid Homo sapiens
12 PCLO  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
13 PPFIA2 8499
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
14 HMOX2 3163
Two-hybrid Homo sapiens
15 PAK2 5062
Affinity Capture-MS Homo sapiens
16 WDR33 55339
Two-hybrid Homo sapiens
17 SRRT 51593
Two-hybrid Homo sapiens
18 PPFIA1 8500
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 ARHGEF7 8874
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 RGS2  
Two-hybrid Homo sapiens
21 MAN2A2  
Two-hybrid Homo sapiens
22 GIT2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
23 SDCCAG3  
Two-hybrid Homo sapiens
24 KIF1A 547
Invivo Homo sapiens
25 PMF1  
Two-hybrid Homo sapiens
26 CAST  
Affinity Capture-Western Homo sapiens
27 TERF1 7013
Two-hybrid Homo sapiens
28 PTPRZ1  
Affinity Capture-Western Homo sapiens
29 GIT1 28964
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
30 PPFIA4  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 DDX24 57062
Two-hybrid Homo sapiens
32 PTK2B 2185
Affinity Capture-Western Homo sapiens
33 TRIB3  
Two-hybrid Homo sapiens
34 PTK2 5747
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 GRIP2 80852
Affinity Capture-Western Homo sapiens
36 ARHGEF6 9459
Affinity Capture-Western Homo sapiens
37 PLCG1 5335
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 HSPC065  
Two-hybrid Homo sapiens
39 ADRBK2  
Invivo Homo sapiens
40 PPFIA3 8541
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GIT1 is involved
PathwayEvidenceSource
Ephrin signaling TAS Reactome





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