Gene description for PPFIA1
Gene name protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Gene symbol PPFIA1
Other names/aliases LIP.1
LIP1
LIPRIN
Species Homo sapiens
 Database cross references - PPFIA1
ExoCarta ExoCarta_8500
Entrez Gene 8500
HGNC 9245
MIM 611054
UniProt Q13136  
 PPFIA1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for PPFIA1
Molecular Function
    signal transducer activity GO:0004871 TAS
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 TAS
    negative regulation of establishment of protein localization to plasma membrane GO:0090005 IMP
    glutamate secretion GO:0014047 TAS
    neurotransmitter secretion GO:0007269 TAS
    synaptic transmission GO:0007268 TAS
    negative regulation of stress fiber assembly GO:0051497 IMP
    cell-matrix adhesion GO:0007160 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    presynaptic active zone GO:0048786 TAS
    focal adhesion GO:0005925 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified PPFIA1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPFIA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPFIBP2 8495
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
2 PPP2R5D  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 PTPRF 5792
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
4 GRIP2 80852
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 PPFIA2 8499
Invitro Homo sapiens
Invivo Homo sapiens
6 CAST  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 PTPRC 5788
Invitro Homo sapiens
8 PPFIBP1 8496
Invitro Homo sapiens
Invivo Homo sapiens
9 NCOA2 10499
Affinity Capture-Western Homo sapiens
10 MBIP  
Two-hybrid Homo sapiens
11 GIT1 28964
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
13 PTPRS 5802
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
14 PPFIA3 8541
Invitro Homo sapiens
15 PTPRD 5789
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
16 RAB6IP2  
Affinity Capture-MS Homo sapiens
17 PPP2CA 5515
Affinity Capture-MS Homo sapiens
18 TNNT1  
Two-hybrid Homo sapiens
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