Gene description for LLGL2
Gene name lethal giant larvae homolog 2 (Drosophila)
Gene symbol LLGL2
Other names/aliases HGL
Hugl-2
LGL2
Species Homo sapiens
 Database cross references - LLGL2
ExoCarta ExoCarta_3993
Vesiclepedia VP_3993
Entrez Gene 3993
HGNC 6629
UniProt Q6P1M3  
 LLGL2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LLGL2
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    PDZ domain binding GO:0030165 IPI
    myosin II binding GO:0045159 IBA
Biological Process
    exocytosis GO:0006887 IEA
    Golgi to plasma membrane transport GO:0006893 IBA
    regulation of Notch signaling pathway GO:0008593 IBA
    L-leucine transport GO:0015820 IMP
    cortical actin cytoskeleton organization GO:0030866 IBA
    regulation of establishment or maintenance of cell polarity GO:0032878 IBA
    regulation of establishment or maintenance of cell polarity GO:0032878 IDA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 NAS
    establishment of spindle orientation GO:0051294 IBA
    cell division GO:0051301 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    adherens junction GO:0005912 NAS
    cortical actin cytoskeleton GO:0030864 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified LLGL2 in exosomes
1
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LLGL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAD18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ZNF668  
Affinity Capture-MS Homo sapiens
3 UBE2O 63893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 LLGL1 3996
Affinity Capture-MS Homo sapiens
5 ATP5S 27109
Affinity Capture-MS Homo sapiens
6 EXO1  
Affinity Capture-MS Homo sapiens
7 VPRBP 9730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 DIEXF  
Affinity Capture-MS Homo sapiens
10 ZNF687  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 KBTBD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 DDA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TAF9 6880
Affinity Capture-MS Homo sapiens
14 GSE1 23199
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 NOLC1 9221
Affinity Capture-MS Homo sapiens
16 C18orf25 147339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 BRWD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 IMP3 55272
Affinity Capture-MS Homo sapiens
19 IMPDH2 3615
Co-fractionation Homo sapiens
20 UBE2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PLEKHG1  
Affinity Capture-MS Homo sapiens
22 DNAJB1 3337
Affinity Capture-MS Homo sapiens
23 KIAA0556  
Affinity Capture-MS Homo sapiens
24 HMG20A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 HMG20B  
Affinity Capture-MS Homo sapiens
26 DOCK1 1793
Affinity Capture-MS Homo sapiens
27 PALLD 23022
Affinity Capture-MS Homo sapiens
28 RCOR3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DAPK2  
Affinity Capture-MS Homo sapiens
30 KIAA1715 80856
Affinity Capture-MS Homo sapiens
31 C1QBP 708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 BAG5 9529
Affinity Capture-MS Homo sapiens
33 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
34 BHLHE41  
Affinity Capture-MS Homo sapiens
35 TRIM26 7726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 IMPACT 55364
Affinity Capture-MS Homo sapiens
37 BAG6 7917
Affinity Capture-MS Homo sapiens
38 ATF2  
Affinity Capture-MS Homo sapiens
39 RNF146  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
40 ALDH18A1 5832
Affinity Capture-MS Homo sapiens
41 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 IWS1  
Affinity Capture-MS Homo sapiens
43 CCNB1 891
Affinity Capture-MS Homo sapiens
44 GCC1 79571
Affinity Capture-MS Homo sapiens
45 NUDC 10726
Affinity Capture-MS Homo sapiens
46 BPTF 2186
Affinity Capture-MS Homo sapiens
47 DNAJA1 3301
Affinity Capture-MS Homo sapiens
48 LYST 1130
Affinity Capture-MS Homo sapiens
49 NKTR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DNM1L 10059
Affinity Capture-MS Homo sapiens
51 ST5 6764
Affinity Capture-MS Homo sapiens
52 CD99L2 83692
Affinity Capture-MS Homo sapiens
53 BCLAF1 9774
Affinity Capture-MS Homo sapiens
54 RBBP6 5930
Affinity Capture-MS Homo sapiens
55 ZNF316  
Affinity Capture-MS Homo sapiens
56 ELMO3 79767
Affinity Capture-MS Homo sapiens
57 C11orf57  
Affinity Capture-MS Homo sapiens
58 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 PRKCZ 5590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SF1 7536
Affinity Capture-MS Homo sapiens
61 PTEN 5728
Synthetic Growth Defect Homo sapiens
62 MAP3K1 4214
Affinity Capture-MS Homo sapiens
63 ARF6 382
Proximity Label-MS Homo sapiens
64 METTL21B  
Affinity Capture-MS Homo sapiens
65 KBTBD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FMN1 342184
Affinity Capture-MS Homo sapiens
67 PRKCI 5584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 FASN 2194
Negative Genetic Homo sapiens
69 BIRC6 57448
Affinity Capture-MS Homo sapiens
70 RBM26 64062
Affinity Capture-MS Homo sapiens
71 ZMYND8 23613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 MTOR 2475
Affinity Capture-MS Homo sapiens
73 PHF21A  
Affinity Capture-MS Homo sapiens
74 FIP1L1 81608
Affinity Capture-MS Homo sapiens
75 DDB1 1642
Affinity Capture-MS Homo sapiens
76 EPB41L4B 54566
Affinity Capture-MS Homo sapiens
77 CACTIN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PPFIA1 8500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 ZC3H3  
Affinity Capture-MS Homo sapiens
80 NAA10 8260
Co-fractionation Homo sapiens
81 NKAP 79576
Affinity Capture-MS Homo sapiens
82 STUB1 10273
Affinity Capture-MS Homo sapiens
83 SUGT1 10910
Affinity Capture-MS Homo sapiens
84 LLGL2 3993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 ZNF707  
Affinity Capture-MS Homo sapiens
86 Prkci  
Affinity Capture-MS Mus musculus
87 ZNF592 9640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 SVIL 6840
Affinity Capture-MS Homo sapiens
89 MKNK1  
Affinity Capture-MS Homo sapiens
90 PARD6G 84552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 NEK7 140609
Affinity Capture-MS Homo sapiens
93 Pard6b  
Affinity Capture-MS Mus musculus
94 ABCA7 10347
Affinity Capture-MS Homo sapiens
95 SQSTM1 8878
Affinity Capture-MS Homo sapiens
96 THRAP3 9967
Affinity Capture-MS Homo sapiens
97 RNF20 56254
Affinity Capture-MS Homo sapiens
98 ELMO2 63916
Affinity Capture-MS Homo sapiens
99 HMGXB4 10042
Affinity Capture-MS Homo sapiens
100 KDM1A 23028
Affinity Capture-MS Homo sapiens
101 GTPBP4 23560
Affinity Capture-MS Homo sapiens
102 PHACTR4 65979
Affinity Capture-MS Homo sapiens
103 PARD6B 84612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 SSSCA1 10534
Affinity Capture-MS Homo sapiens
105 CEP78 84131
Affinity Capture-MS Homo sapiens
106 EPHA2 1969
Proximity Label-MS Homo sapiens
107 TOPORS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 PTGES3 10728
Affinity Capture-MS Homo sapiens
109 KRAS 3845
Proximity Label-MS Homo sapiens
110 CASC3  
Affinity Capture-MS Homo sapiens
111 UBA6 55236
Co-fractionation Homo sapiens
112 PARD6A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 RCOR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 TIMM13 26517
Affinity Capture-MS Homo sapiens
115 ZNHIT3  
Affinity Capture-MS Homo sapiens
116 ECSIT 51295
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which LLGL2 is involved
No pathways found





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