Gene description for PHACTR4
Gene name phosphatase and actin regulator 4
Gene symbol PHACTR4
Other names/aliases PPP1R124
Species Homo sapiens
 Database cross references - PHACTR4
ExoCarta ExoCarta_65979
Entrez Gene 65979
HGNC 25793
MIM 608726
UniProt Q8IZ21  
 PHACTR4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PHACTR4
Molecular Function
    actin binding GO:0003779 ISS
    protein phosphatase type 1 activator activity GO:0071862 ISS
    protein phosphatase 1 binding GO:0008157 ISS
Biological Process
    neural crest cell migration GO:0001755 ISS
    closure of optic fissure GO:0061386 ISS
    positive regulation of catalytic activity GO:0043085 ISS
    Rho protein signal transduction GO:0007266 ISS
    enteric nervous system development GO:0048484 ISS
    neural tube closure GO:0001843 ISS
    regulation of cell cycle GO:0051726 ISS
    actin cytoskeleton organization GO:0030036 ISS
    negative regulation of integrin-mediated signaling pathway GO:2001045 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    lamellipodium GO:0030027 ISS
 Experiment description of studies that identified PHACTR4 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHACTR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PHACTR4 is involved
No pathways found





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