Gene description for UBE2I
Gene name ubiquitin-conjugating enzyme E2I
Gene symbol UBE2I
Other names/aliases C358B7.1
P18
UBC9
Species Homo sapiens
 Database cross references - UBE2I
ExoCarta ExoCarta_7329
Vesiclepedia VP_7329
Entrez Gene 7329
HGNC 12485
MIM 601661
UniProt P63279  
 UBE2I identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for UBE2I
Molecular Function
    enzyme binding GO:0019899 IPI
    ligase activity GO:0016874 IEA
    SUMO conjugating enzyme activity GO:0061656 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    RING-like zinc finger domain binding GO:0071535 IPI
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    protein C-terminus binding GO:0008022 IEA
    transcription factor binding GO:0008134 IPI
    SUMO transferase activity GO:0019789 EXP
    HLH domain binding GO:0043398 IEA
    bHLH transcription factor binding GO:0043425 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    positive regulation of SUMO transferase activity GO:1903755 IDA
    cellular protein modification process GO:0006464 TAS
    regulation of receptor activity GO:0010469 IEA
    chromosome segregation GO:0007059 IEA
    mitotic nuclear division GO:0007067 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IMP
    cell division GO:0051301 IEA
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IEA
    protein ubiquitination GO:0016567 IBA
    viral process GO:0016032 IEA
    positive regulation of intracellular steroid hormone receptor signaling pathway GO:0033145 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    protein sumoylation GO:0016925 TAS
    post-translational protein modification GO:0043687 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IDA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IDA
    sumoylated E2 ligase complex GO:1990356 IC
    fibrillar center GO:0001650 IEA
    transferase complex GO:1990234 IDA
    PML body GO:0016605 IDA
    synapse GO:0045202 IEA
    synaptonemal complex GO:0000795 TAS
    cytoplasm GO:0005737 IDA
    dendrite GO:0030425 IEA
 Experiment description of studies that identified UBE2I in exosomes
1
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBE2I
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Affinity Capture-MS Homo sapiens
2 MAPK1IP1L 93487
Two-hybrid Homo sapiens
3 TRIM63  
Two-hybrid Homo sapiens
4 CBX4  
Affinity Capture-MS Homo sapiens
5 HNRNPC 3183
Two-hybrid Homo sapiens
6 ZBED1  
Two-hybrid Homo sapiens
7 MITF  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
8 ZNFN1A1  
Two-hybrid Homo sapiens
9 SAE1 10055
Affinity Capture-MS Homo sapiens
10 TRIM29 23650
Two-hybrid Homo sapiens
11 SCNN1A  
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 TP53 7157
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 TRAF4 9618
Affinity Capture-MS Homo sapiens
14 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 GMEB2  
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 PDLIM7 9260
Two-hybrid Homo sapiens
17 FMR1 2332
Two-hybrid Homo sapiens
18 C18orf25 147339
Two-hybrid Homo sapiens
19 PPM1J  
Reconstituted Complex Homo sapiens
20 ATF2  
Two-hybrid Homo sapiens
Far Western Homo sapiens
21 PIAS2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
22 TDP2 51567
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
23 SREBF1 6720
Affinity Capture-Western Homo sapiens
24 RAD52  
Two-hybrid Homo sapiens
25 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
26 UNC119 9094
Two-hybrid Homo sapiens
27 NR3C1 2908
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 DACH1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
29 PTEN 5728
Affinity Capture-Western Homo sapiens
30 SMAD4  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
31 TRIM55  
Two-hybrid Homo sapiens
32 PRPF40A 55660
Affinity Capture-MS Homo sapiens
33 SALL1 6299
Invitro Homo sapiens
Two-hybrid Homo sapiens
34 ETS1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
35 TP73  
Two-hybrid Homo sapiens
36 HNRNPK 3190
Two-hybrid Homo sapiens
37 TRIM54  
Two-hybrid Homo sapiens
38 DNMT3A 1788
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 HSF2  
Two-hybrid Homo sapiens
40 MAP3K1 4214
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
41 APEX1 328
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
42 JUN 3725
Two-hybrid Homo sapiens
43 DAXX  
Two-hybrid Homo sapiens
44 NUDCD3  
Two-hybrid Homo sapiens
45 SNIP1  
Affinity Capture-MS Homo sapiens
46 DNMT3B  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
47 UBA2 10054
Affinity Capture-MS Homo sapiens
48 TOP1 7150
Invivo Homo sapiens
Invitro Homo sapiens
49 CHMP4B 128866
Two-hybrid Homo sapiens
50 SREBF2 6721
Affinity Capture-Western Homo sapiens
51 IPO13 9670
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
52 RIPK2  
Two-hybrid Homo sapiens
53 RAD54B  
Two-hybrid Homo sapiens
54 HIPK2 28996
Two-hybrid Homo sapiens
55 WT1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
56 PHC1  
Two-hybrid Homo sapiens
57 GMEB1  
Invitro Homo sapiens
Two-hybrid Homo sapiens
58 TCF3  
Invivo Homo sapiens
59 BLMH 642
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
60 SUMO2 6613
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
61 STAT1 6772
Affinity Capture-MS Homo sapiens
62 TOPORS  
Two-hybrid Homo sapiens
63 ZNFN1A3  
Two-hybrid Homo sapiens
64 HGS 9146
Two-hybrid Homo sapiens
65 FHIT 2272
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
66 PIAS1 8554
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
67 SUMO3 6612
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 AR 367
Invitro Homo sapiens
Two-hybrid Homo sapiens
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
69 PDZK1 5174
Two-hybrid Homo sapiens
70 SUMO1 7341
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
71 MYB  
Invitro Homo sapiens
Two-hybrid Homo sapiens
72 ZNF451 26036
Two-hybrid Homo sapiens
73 RAD51  
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
74 UCHL1 7345
Two-hybrid Homo sapiens
75 RANGAP1 5905
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
76 RANBP2 5903
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
77 CHD3 1107
Two-hybrid Homo sapiens
78 SATB1 6304
Two-hybrid Homo sapiens
79 ZNF646  
Two-hybrid Homo sapiens
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