Gene description for DNMT3A
Gene name DNA (cytosine-5-)-methyltransferase 3 alpha
Gene symbol DNMT3A
Other names/aliases DNMT3A2
M.HsaIIIA
TBRS
Species Homo sapiens
 Database cross references - DNMT3A
ExoCarta ExoCarta_1788
Entrez Gene 1788
HGNC 2978
MIM 602769
UniProt Q9Y6K1  
 DNMT3A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for DNMT3A
Molecular Function
    DNA (cytosine-5-)-methyltransferase activity GO:0003886 IDA
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IDA
    DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates GO:0051718 IEA
    chromatin binding GO:0003682 IEA
    identical protein binding GO:0042802 IPI
    unmethylated CpG binding GO:0045322 IEA
    metal ion binding GO:0046872 IEA
    DNA-methyltransferase activity GO:0009008 TAS
Biological Process
    C-5 methylation of cytosine GO:0090116 IEA
    hypermethylation of CpG island GO:0044027 IEA
    S-adenosylmethioninamine metabolic process GO:0046499 IEA
    regulation of gene expression, epigenetic GO:0040029 TAS
    regulation of gene expression by genetic imprinting GO:0006349 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    DNA methylation on cytosine GO:0032776 TAS
    S-adenosylhomocysteine metabolic process GO:0046498 IEA
    methylation-dependent chromatin silencing GO:0006346 IEA
    chromatin organization GO:0006325 TAS
    DNA methylation on cytosine within a CG sequence GO:0010424 IEA
    DNA methylation involved in embryo development GO:0043045 IEA
    gene expression GO:0010467 TAS
    DNA methylation involved in gamete generation GO:0043046 IEA
    cellular response to amino acid stimulus GO:0071230 IEA
    spermatogenesis GO:0007283 IEA
    DNA methylation GO:0006306 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    euchromatin GO:0000791 IDA
    nuclear heterochromatin GO:0005720 IEA
    nuclear matrix GO:0016363 IDA
    nucleus GO:0005634 IDA
    chromosome, centromeric region GO:0000775 IEA
    nucleoplasm GO:0005654 TAS
    XY body GO:0001741 IEA
 Experiment description of studies that identified DNMT3A in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for DNMT3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF238  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 DNMT3L  
Affinity Capture-Western Homo sapiens
3 SUV39H1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 UBE2I 7329
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 ZBTB17  
Affinity Capture-Western Homo sapiens
6 CBX1 10951
Reconstituted Complex Homo sapiens
7 DNMT3B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 PIAS1 8554
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 MYC  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 DNMT1 1786
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 Zfp238  
Two-hybrid Mus musculus
12 HDAC1 3065
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 RB1 5925
Reconstituted Complex Homo sapiens
14 CBX5 23468
Affinity Capture-Western Homo sapiens
15 PIAS2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 RPS8 6202
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which DNMT3A is involved
PathwayEvidenceSource
DNA methylation TAS Reactome
PRC2 methylates histones and DNA TAS Reactome
RMTs methylate histone arginines TAS Reactome





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