Gene description for UHRF1
Gene name ubiquitin-like with PHD and ring finger domains 1
Gene symbol UHRF1
Other names/aliases ICBP90
Np95
RNF106
hNP95
hUHRF1
huNp95
Species Homo sapiens
 Database cross references - UHRF1
ExoCarta ExoCarta_29128
Vesiclepedia VP_29128
Entrez Gene 29128
HGNC 12556
MIM 607990
UniProt Q96T88  
 UHRF1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for UHRF1
Molecular Function
    cis-regulatory region sequence-specific DNA binding GO:0000987 IDA
    nucleic acid binding GO:0003676 EXP
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
    methyl-CpG binding GO:0008327 IDA
    methylated histone binding GO:0035064 IDA
    histone binding GO:0042393 IDA
    histone binding GO:0042393 ISS
    identical protein binding GO:0042802 ISS
    hemi-methylated DNA-binding GO:0044729 IDA
    hemi-methylated DNA-binding GO:0044729 TAS
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IDA
    ubiquitin protein ligase activity GO:0061630 ISS
    H3K9me3 modified histone binding GO:0062072 TAS
    DNA damage sensor activity GO:0140612 TAS
    histone H3 ubiquitin ligase activity GO:0141055 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    double-strand break repair via homologous recombination GO:0000724 TAS
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    DNA damage response GO:0006974 TAS
    protein ubiquitination GO:0016567 IBA
    heterochromatin formation GO:0031507 IDA
    homologous recombination GO:0035825 TAS
    epigenetic regulation of gene expression GO:0040029 TAS
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IBA
    negative regulation of gene expression via chromosomal CpG island methylation GO:0044027 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IC
    regulation of epithelial cell proliferation GO:0050678 IMP
    positive regulation of protein metabolic process GO:0051247 IDA
    protein autoubiquitination GO:0051865 IDA
    mitotic spindle assembly GO:0090307 IDA
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 IC
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISS
    euchromatin GO:0000791 IDA
    heterochromatin GO:0000792 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    replication fork GO:0005657 IDA
    spindle GO:0005819 IC
    nuclear matrix GO:0016363 ISS
 Experiment description of studies that identified UHRF1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UHRF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 DPPA3  
Affinity Capture-MS Homo sapiens
5 KPNA1 3836
Affinity Capture-MS Homo sapiens
6 RNF4 6047
Affinity Capture-Western Homo sapiens
7 ZPR1 8882
Co-fractionation Homo sapiens
8 MBD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 DBN1 1627
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Affinity Capture-Western Homo sapiens
11 EME1  
Affinity Capture-Western Homo sapiens
12 RPL10 6134
Affinity Capture-MS Homo sapiens
13 UQCRB 7381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ANKRD39  
Affinity Capture-MS Homo sapiens
15 KAT5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 MILR1  
Affinity Capture-MS Homo sapiens
17 CENPA  
Proximity Label-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 TOP2A 7153
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 TRIM28 10155
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 PRC1 9055
Affinity Capture-MS Homo sapiens
22 KIF23 9493
Affinity Capture-MS Homo sapiens
23 LIG1 3978
Protein-peptide Homo sapiens
24 HIST2H3A 333932
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
25 FBXW7  
Affinity Capture-MS Homo sapiens
26 LASP1 3927
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 SUV39H1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 OBSL1 23363
Affinity Capture-MS Homo sapiens
29 HMGA1 3159
Affinity Capture-MS Homo sapiens
30 ATP2A3 489
Affinity Capture-MS Homo sapiens
31 BRD4 23476
Affinity Capture-MS Homo sapiens
32 Mbd4  
Affinity Capture-Western Mus musculus
33 PSD2  
Affinity Capture-MS Homo sapiens
34 CUL1 8454
Affinity Capture-Western Homo sapiens
35 BTRC 8945
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 MYCN  
Affinity Capture-MS Homo sapiens
37 USP7 7874
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 RB1 5925
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 E2F4  
Affinity Capture-MS Homo sapiens
41 AURKA 6790
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
43 CBX3 11335
Proximity Label-MS Homo sapiens
44 USP11 8237
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
45 TCEAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 DNMT3A 1788
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
48 TBC1D32 221322
Proximity Label-MS Homo sapiens
49 KDM4B  
Affinity Capture-MS Homo sapiens
50 Srp72  
Affinity Capture-MS Mus musculus
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 FAM24B  
Affinity Capture-MS Homo sapiens
53 RAD18  
Affinity Capture-MS Homo sapiens
54 WIPI1 55062
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 AOC4P  
Affinity Capture-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
56 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
57 ALYREF 10189
Affinity Capture-RNA Homo sapiens
58 PML 5371
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 UBE2D3 7323
Reconstituted Complex Homo sapiens
60 HECTD1 25831
Affinity Capture-MS Homo sapiens
61 SETD7 80854
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
62 DAXX  
Affinity Capture-MS Homo sapiens
63 UBXN6 80700
Affinity Capture-MS Homo sapiens
64 UBE2N 7334
Reconstituted Complex Homo sapiens
65 S100P 6286
Affinity Capture-MS Homo sapiens
66 TRPV5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 HIST1H3A 8350
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
68 FANCD2  
Affinity Capture-MS Homo sapiens
69 HBS1L 10767
Affinity Capture-MS Homo sapiens
70 MUS81  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
71 HIST2H3C 126961
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
72 LMNB1 4001
Proximity Label-MS Homo sapiens
73 BKRF4  
Affinity Capture-MS
74 BRD2  
Affinity Capture-MS Homo sapiens
75 CD274 29126
Affinity Capture-MS Homo sapiens
76 C19orf66  
Affinity Capture-MS Homo sapiens
77 ERCC4  
Affinity Capture-Western Homo sapiens
78 CUL3 8452
Affinity Capture-MS Homo sapiens
79 ATP5F1 515
Affinity Capture-MS Homo sapiens
80 UHRF2  
Affinity Capture-MS Homo sapiens
81 EGFR 1956
Negative Genetic Homo sapiens
82 ERCC1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
83 METTL21B  
Affinity Capture-MS Homo sapiens
84 SERINC5 256987
Affinity Capture-MS Homo sapiens
85 CCDC22 28952
Affinity Capture-MS Homo sapiens
86 SUZ12  
Affinity Capture-Western Homo sapiens
87 HIST3H3 8290
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Protein-peptide Homo sapiens
88 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
89 CCNDBP1 23582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 ZNF18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 PRKACA 5566
Biochemical Activity Homo sapiens
92 DNMT3B  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
93 MYC  
Affinity Capture-MS Homo sapiens
94 VIM 7431
Co-fractionation Homo sapiens
95 UHRF1BP1 54887
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
96 RPL35A 6165
Affinity Capture-MS Homo sapiens
97 EHMT2 10919
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
98 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
99 RNF2  
Affinity Capture-MS Homo sapiens
100 Rpl35 66489
Affinity Capture-MS Mus musculus
101 HNRNPU 3192
Affinity Capture-MS Homo sapiens
102 NFATC2  
Affinity Capture-MS Homo sapiens
103 BRD3 8019
Affinity Capture-MS Homo sapiens
104 CCDC8  
Affinity Capture-MS Homo sapiens
105 UBE2D1 7321
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
106 AHSA1 10598
Affinity Capture-MS Homo sapiens
107 PADI6  
Affinity Capture-MS Homo sapiens
108 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
109 FBXW11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
110 PARP1 142
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 TP53 7157
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
112 Pcgf1  
Affinity Capture-MS Mus musculus
113 SSRP1 6749
Affinity Capture-MS Homo sapiens
114 DDX3X 1654
Co-fractionation Homo sapiens
115 WRAP53  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
116 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
117 HDAC1 3065
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
118 PRMT5 10419
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
119 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
120 MKI67  
Affinity Capture-MS Homo sapiens
121 PARP2  
Affinity Capture-Western Homo sapiens
122 PCNA 5111
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
123 ZNF691  
Affinity Capture-MS Homo sapiens
124 RBM4 5936
Affinity Capture-MS Homo sapiens
125 HIST2H3D 653604
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
126 EZH2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
127 RNMTL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 H2AFZ 3015
Affinity Capture-MS Homo sapiens
129 Rrbp1  
Affinity Capture-MS Mus musculus
130 ERG  
Reconstituted Complex Homo sapiens
131 PTGES3 10728
Affinity Capture-MS Homo sapiens
132 KRAS 3845
Negative Genetic Homo sapiens
133 UHRF1 29128
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
134 TMCC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 NUCKS1 64710
Affinity Capture-MS Homo sapiens
136 DNMT1 1786
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
137 FBXO38 81545
Proximity Label-MS Homo sapiens
138 ZNF467  
Affinity Capture-MS Homo sapiens
139 KDM1A 23028
Affinity Capture-Western Homo sapiens
140 ARIH1 25820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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