Gene description for UHRF1
Gene name ubiquitin-like with PHD and ring finger domains 1
Gene symbol UHRF1
Other names/aliases ICBP90
Np95
RNF106
hNP95
hUHRF1
huNp95
Species Homo sapiens
 Database cross references - UHRF1
ExoCarta ExoCarta_29128
Vesiclepedia VP_29128
Entrez Gene 29128
HGNC 12556
MIM 607990
UniProt Q96T88  
 UHRF1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for UHRF1
Molecular Function
    nucleosomal histone binding GO:0031493 ISS
    ligase activity GO:0016874 IEA
    hemi-methylated DNA-binding GO:0044729 IDA
    ubiquitin protein ligase activity GO:0061630 ISS
    ubiquitin-protein transferase activity GO:0004842 IDA
    methylated histone binding GO:0035064 IDA
    methyl-CpG binding GO:0008327 IDA
    zinc ion binding GO:0008270 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    identical protein binding GO:0042802 ISS
    protein binding GO:0005515 IPI
    core promoter proximal region sequence-specific DNA binding GO:0000987 IDA
    histone binding GO:0042393 IDA
Biological Process
    cell cycle GO:0007049 IEA
    regulation of gene expression, epigenetic GO:0040029 TAS
    protein autoubiquitination GO:0051865 IDA
    positive regulation of cellular protein metabolic process GO:0032270 IDA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IC
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 IC
    transcription, DNA-templated GO:0006351 IEA
    histone ubiquitination GO:0016574 IDA
    cell proliferation GO:0008283 IEP
    DNA repair GO:0006281 IEA
    gene expression GO:0010467 TAS
    histone monoubiquitination GO:0010390 ISS
    DNA methylation on cytosine GO:0032776 TAS
    maintenance of DNA methylation GO:0010216 IMP
Subcellular Localization
    heterochromatin GO:0000792 IDA
    nuclear matrix GO:0016363 ISS
    nuclear chromatin GO:0000790 ISS
    euchromatin GO:0000791 IDA
    replication fork GO:0005657 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified UHRF1 in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UHRF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which UHRF1 is involved
PathwayEvidenceSource
DNA methylation TAS Reactome





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