Gene description for HBS1L
Gene name HBS1-like translational GTPase
Gene symbol HBS1L
Other names/aliases EF-1a
ERFS
HBS1
HSPC276
eRF3c
Species Homo sapiens
 Database cross references - HBS1L
ExoCarta ExoCarta_10767
Vesiclepedia VP_10767
Entrez Gene 10767
HGNC 4834
MIM 612450
UniProt Q9Y450  
 HBS1L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for HBS1L
Molecular Function
    translation elongation factor activity GO:0003746 IEA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
Biological Process
    translation GO:0006412 IBA
    regulation of translation GO:0006417 IEA
    signal transduction GO:0007165 TAS
    ribosome disassembly GO:0032790 IDA
    nuclear-transcribed mRNA catabolic process, no-go decay GO:0070966 IDA
    rescue of stalled ribosome GO:0072344 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    cytosolic ribosome GO:0022626 IDA
    extracellular exosome GO:0070062 HDA
    Dom34-Hbs1 complex GO:1990533 IDA
 Experiment description of studies that identified HBS1L in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HBS1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EXOSC7 23016
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 STK25 10494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 LAMP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SRPK2 6733
Biochemical Activity Homo sapiens
5 EPB41L5 57669
Affinity Capture-MS Homo sapiens
6 C9orf78 51759
Affinity Capture-MS Homo sapiens
7 FAXC  
Affinity Capture-MS Homo sapiens
8 EIF4E2  
Affinity Capture-MS Homo sapiens
9 APP 351
Reconstituted Complex Homo sapiens
10 GCHFR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 UBE2G2  
Two-hybrid Homo sapiens
12 APEX1 328
Affinity Capture-RNA Homo sapiens
13 PSIP1 11168
Co-fractionation Homo sapiens
14 RPS20 6224
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
15 EXOSC2 23404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C11orf87  
Affinity Capture-MS Homo sapiens
17 SLC31A1 1317
Affinity Capture-MS Homo sapiens
18 EXOSC4 54512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
19 GOPC 57120
Affinity Capture-MS Homo sapiens
20 CPLX1 10815
Co-fractionation Homo sapiens
21 EXOSC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
22 Rpl35 66489
Affinity Capture-MS Mus musculus
23 EXOSC6 118460
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 RAB39B 116442
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MPHOSPH6 10200
Affinity Capture-MS Homo sapiens
26 FAS 355
Proximity Label-MS Homo sapiens
27 EIF2S3 1968
Co-fractionation Homo sapiens
28 DIS3L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 JMJD6 23210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PELO 53918
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 PIAS4  
Affinity Capture-MS Homo sapiens
33 MSX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RBM7  
Affinity Capture-MS Homo sapiens
35 DTYMK 1841
Affinity Capture-MS Homo sapiens
36 PRPS2 5634
Affinity Capture-MS Homo sapiens
37 HTT 3064
Affinity Capture-MS Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 EXOSC5 56915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
40 TULP3 7289
Affinity Capture-MS Homo sapiens
41 RPA4  
Proximity Label-MS Homo sapiens
42 SPDYE4  
Affinity Capture-MS Homo sapiens
43 Exosc1  
Affinity Capture-MS Mus musculus
44 SRPK1 6732
Biochemical Activity Homo sapiens
45 PIPSL 266971
Affinity Capture-MS Homo sapiens
46 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
47 CXCR4 7852
Affinity Capture-MS Homo sapiens
48 AIFM1 9131
Affinity Capture-MS Homo sapiens
49 XPO1 7514
Affinity Capture-MS Homo sapiens
50 GPX1 2876
Affinity Capture-MS Homo sapiens
51 CD6 923
Affinity Capture-MS Homo sapiens
52 CUL3 8452
Affinity Capture-MS Homo sapiens
53 MAP3K1 4214
Biochemical Activity Homo sapiens
54 FAM90A1  
Affinity Capture-MS Homo sapiens
55 CCDC85A  
Affinity Capture-MS Homo sapiens
56 HOOK3 84376
Proximity Label-MS Homo sapiens
57 LIN9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 EXOSC3 51010
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
60 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
61 DDRGK1 65992
Affinity Capture-MS Homo sapiens
62 CACNG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 HUWE1 10075
Affinity Capture-MS Homo sapiens
64 RPS16 6217
Affinity Capture-MS Homo sapiens
65 SLC16A1 6566
Cross-Linking-MS (XL-MS) Homo sapiens
66 EXOSC10 5394
Affinity Capture-MS Homo sapiens
67 EIF1AD 84285
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SURF6  
Affinity Capture-MS Homo sapiens
69 GIGYF2 26058
Affinity Capture-MS Homo sapiens
70 SIRT6  
Affinity Capture-MS Homo sapiens
71 TP53 7157
Affinity Capture-MS Homo sapiens
72 DNAJA2 10294
Affinity Capture-MS Homo sapiens
73 ISCA2 122961
Affinity Capture-MS Homo sapiens
74 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 C1orf35  
Affinity Capture-MS Homo sapiens
76 FANCI 55215
Co-fractionation Homo sapiens
77 EXOSC9 5393
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
78 RPS5 6193
Co-fractionation Homo sapiens
79 C16orf72 29035
Affinity Capture-MS Homo sapiens
80 LIPA 3988
Affinity Capture-MS Homo sapiens
81 EP300 2033
Affinity Capture-MS Homo sapiens
82 PTGES3 10728
Affinity Capture-MS Homo sapiens
83 BICD2 23299
Proximity Label-MS Homo sapiens
84 UHRF1 29128
Affinity Capture-MS Homo sapiens
85 HS1BP3 64342
Affinity Capture-MS Homo sapiens
86 EXOSC1 51013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
87 FAM133A 286499
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HBS1L is involved
PathwayEvidenceSource
Deadenylation-dependent mRNA decay TAS Reactome
Metabolism of RNA TAS Reactome
mRNA decay by 3' to 5' exoribonuclease TAS Reactome





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