Gene description for DTYMK
Gene name deoxythymidylate kinase (thymidylate kinase)
Gene symbol DTYMK
Other names/aliases CDC8
PP3731
TMPK
TYMK
Species Homo sapiens
 Database cross references - DTYMK
ExoCarta ExoCarta_1841
Entrez Gene 1841
HGNC 3061
MIM 188345
UniProt P23919  
 DTYMK identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for DTYMK
Molecular Function
    thymidylate kinase activity GO:0004798 IDA
    uridylate kinase activity GO:0009041 IBA
    nucleoside phosphate kinase activity GO:0050145 EXP
    ATP binding GO:0005524 IEA
Biological Process
    response to cadmium ion GO:0046686 IEA
    nucleobase-containing small molecule interconversion GO:0015949 TAS
    nucleotide phosphorylation GO:0046939 TAS
    cell cycle GO:0007049 TAS
    nucleoside monophosphate phosphorylation GO:0046940 EXP
    myoblast differentiation GO:0045445 IEA
    response to estrogen GO:0043627 IEA
    small molecule metabolic process GO:0044281 TAS
    cellular response to growth factor stimulus GO:0071363 IEA
    dTTP biosynthetic process GO:0006235 IEA
    dUDP biosynthetic process GO:0006227 IBA
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    dTDP biosynthetic process GO:0006233 IBA
    cell proliferation GO:0008283 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrial intermembrane space GO:0005758 IEA
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified DTYMK in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DTYMK
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DTYMK is involved
PathwayEvidenceSource
Synthesis and interconversion of nucleotide di- and triphosphates TAS Reactome





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