Gene description for ATP6AP2
Gene name ATPase, H+ transporting, lysosomal accessory protein 2
Gene symbol ATP6AP2
Other names/aliases APT6M8-9
ATP6IP2
ATP6M8-9
ELDF10
HT028
M8-9
MRXE
MRXSH
MSTP009
PRR
RENR
XMRE
XPDS
Species Homo sapiens
 Database cross references - ATP6AP2
ExoCarta ExoCarta_10159
Vesiclepedia VP_10159
Entrez Gene 10159
HGNC 18305
MIM 300556
UniProt O75787  
 ATP6AP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25857301    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for ATP6AP2
Molecular Function
    protein binding GO:0005515 IPI
    signaling receptor activity GO:0038023 IEA
Biological Process
    angiotensin maturation GO:0002003 IDA
    vacuolar acidification GO:0007035 NAS
    lysosomal lumen acidification GO:0007042 IMP
    lysosomal lumen acidification GO:0007042 NAS
    central nervous system maturation GO:0021626 IMP
    rostrocaudal neural tube patterning GO:0021903 IMP
    positive regulation of Wnt signaling pathway GO:0030177 IBA
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    positive regulation of transforming growth factor beta1 production GO:0032914 IDA
    regulation of MAPK cascade GO:0043408 IDA
    eye pigmentation GO:0048069 IMP
    endosomal lumen acidification GO:0048388 NAS
    intracellular pH reduction GO:0051452 NAS
    head morphogenesis GO:0060323 IMP
    Golgi lumen acidification GO:0061795 NAS
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    synaptic vesicle lumen acidification GO:0097401 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 ISS
    autophagosome membrane GO:0000421 IEA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 ISS
    lysosomal membrane GO:0005765 NAS
    endoplasmic reticulum membrane GO:0005789 IDA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    endosome membrane GO:0010008 ISS
    endosome membrane GO:0010008 NAS
    membrane GO:0016020 IDA
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IMP
    axon GO:0030424 IEA
    clathrin-coated vesicle membrane GO:0030665 IEA
    synaptic vesicle membrane GO:0030672 IEA
    dendritic spine membrane GO:0032591 IEA
    proton-transporting V-type ATPase complex GO:0033176 NAS
    postsynaptic membrane GO:0045211 IEA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified ATP6AP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
10
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
14
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 250
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|TSG101|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857301    
Organism Homo sapiens
Experiment description Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles.
Authors "Minciacchi VR, You S, Spinelli C, Morley S, Zandian M, Aspuria PJ, Cavallini L, Ciardiello C, Reis Sobreiro M, Morello M, Kharmate G, Jang SC, Kim DK, Hosseini-Beheshti E, Tomlinson Guns E, Gleave M, Gho YS, Mathivanan S, Yang W, Freeman MR, Di Vizio D"
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - expressing DIAPH3 hRNA
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
22
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATP6AP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 CHMP2A 27243
Affinity Capture-MS Homo sapiens
3 CAPNS2 84290
Affinity Capture-MS Homo sapiens
4 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 RIT1 6016
Negative Genetic Homo sapiens
6 HSD17B11 51170
Proximity Label-MS Homo sapiens
7 CASP14 23581
Affinity Capture-MS Homo sapiens
8 AGT 183
Biochemical Activity Homo sapiens
9 SERPINB4 6318
Affinity Capture-MS Homo sapiens
10 ZBTB11  
Affinity Capture-MS Homo sapiens
11 MITD1 129531
Affinity Capture-MS Homo sapiens
12 METTL7A 25840
Proximity Label-MS Homo sapiens
13 LAMP3  
Proximity Label-MS Homo sapiens
14 CPM 1368
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CMTM8  
Affinity Capture-MS Homo sapiens
16 SH3BP4 23677
Affinity Capture-MS Homo sapiens
17 ACTR2 10097
Affinity Capture-MS Homo sapiens
18 BEGAIN  
Affinity Capture-MS Homo sapiens
19 CD72  
Two-hybrid Homo sapiens
20 KIAA2013 90231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RAB11B 9230
Affinity Capture-MS Homo sapiens
22 GLB1 2720
Affinity Capture-MS Homo sapiens
23 JPH1 56704
Affinity Capture-MS Homo sapiens
24 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
25 CD2AP 23607
Affinity Capture-MS Homo sapiens
26 PLBD1 79887
Affinity Capture-MS Homo sapiens
27 SLC2A1 6513
Affinity Capture-MS Homo sapiens
28 TMEM203  
Two-hybrid Homo sapiens
29 COPB2 9276
Affinity Capture-MS Homo sapiens
30 AGA 175
Affinity Capture-MS Homo sapiens
31 GJD3  
Proximity Label-MS Homo sapiens
32 DNAJC25 548645
Proximity Label-MS Homo sapiens
33 BASP1 10409
Affinity Capture-MS Homo sapiens
34 SERPINA3 12
Affinity Capture-MS Homo sapiens
35 PTPN1 5770
Proximity Label-MS Homo sapiens
36 AZGP1 563
Affinity Capture-MS Homo sapiens
37 ALOX12B  
Affinity Capture-MS Homo sapiens
38 S100P 6286
Affinity Capture-MS Homo sapiens
39 URB2  
Affinity Capture-MS Homo sapiens
40 SERINC2 347735
Affinity Capture-MS Homo sapiens
41 APEX1 328
Affinity Capture-RNA Homo sapiens
42 CLCN7 1186
Affinity Capture-MS Homo sapiens
43 FIS1 51024
Proximity Label-MS Homo sapiens
44 GSDMA 284110
Affinity Capture-MS Homo sapiens
45 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
46 PRKCDBP 112464
Affinity Capture-MS Homo sapiens
47 ATP6V1G1 9550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SDHB 6390
Co-fractionation Homo sapiens
49 CHI3L1 1116
Affinity Capture-MS Homo sapiens
50 TIMMDC1  
Two-hybrid Homo sapiens
51 RAB3B 5865
Proximity Label-MS Homo sapiens
52 TFRC 7037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 VKORC1 79001
Two-hybrid Homo sapiens
54 STX7 8417
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ST8SIA5  
Affinity Capture-MS Homo sapiens
56 SSR1 6745
Proximity Label-MS Homo sapiens
57 IL37  
Affinity Capture-MS Homo sapiens
58 CHMP2B 25978
Affinity Capture-MS Homo sapiens
59 MTUS2 23281
Affinity Capture-MS Homo sapiens
60 KLK10 5655
Affinity Capture-MS Homo sapiens
61 FLG 2312
Affinity Capture-MS Homo sapiens
62 KRAS 3845
Negative Genetic Homo sapiens
63 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 TMEM97 27346
Two-hybrid Homo sapiens
65 CPA4 51200
Affinity Capture-MS Homo sapiens
66 KPRP 448834
Affinity Capture-MS Homo sapiens
67 CCDC47 57003
Affinity Capture-MS Homo sapiens
68 CCDC115 84317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TYMP 1890
Affinity Capture-MS Homo sapiens
71 RAB35 11021
Proximity Label-MS Homo sapiens
72 TMEM106A  
Affinity Capture-MS Homo sapiens
73 RHOG 391
Affinity Capture-MS Homo sapiens
74 MARCKS 4082
Proximity Label-MS Homo sapiens
75 STX12 23673
Affinity Capture-MS Homo sapiens
76 TES 26136
Affinity Capture-MS Homo sapiens
77 ADRA1A  
Two-hybrid Homo sapiens
78 KIF14 9928
Affinity Capture-MS Homo sapiens
79 CTSV 1515
Affinity Capture-MS Homo sapiens
80 KIDINS220 57498
Affinity Capture-MS Homo sapiens
81 WDR1 9948
Co-fractionation Homo sapiens
82 LMAN1 3998
Proximity Label-MS Homo sapiens
83 SCPEP1 59342
Affinity Capture-MS Homo sapiens
84 NUP155 9631
Proximity Label-MS Homo sapiens
85 UNK  
Affinity Capture-RNA Homo sapiens
86 PXMP2  
Proximity Label-MS Homo sapiens
87 RAB5A 5868
Proximity Label-MS Homo sapiens
88 RHOA 387
Affinity Capture-MS Homo sapiens
89 LMNB1 4001
Proximity Label-MS Homo sapiens
90 POF1B 79983
Affinity Capture-MS Homo sapiens
91 SCFD1 23256
Affinity Capture-MS Homo sapiens
92 TOP3B 8940
Affinity Capture-MS Homo sapiens
93 ACPP 55
Affinity Capture-MS Homo sapiens
94 EMD 2010
Proximity Label-MS Homo sapiens
95 RAB14 51552
Affinity Capture-MS Homo sapiens
96 NSG1  
Two-hybrid Homo sapiens
97 ZBTB16  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
98 CST6  
Affinity Capture-MS Homo sapiens
99 COPE 11316
Affinity Capture-MS Homo sapiens
100 SBSN 374897
Affinity Capture-MS Homo sapiens
101 RALA 5898
Affinity Capture-MS Homo sapiens
102 PLD1 5337
Affinity Capture-MS Homo sapiens
103 DTYMK 1841
Co-fractionation Homo sapiens
104 IL36G 56300
Affinity Capture-MS Homo sapiens
105 CERS2 29956
Affinity Capture-MS Homo sapiens
106 UBAC2 337867
Affinity Capture-MS Homo sapiens
107 CHRNB2  
Affinity Capture-MS Homo sapiens
108 PILRB 29990
Affinity Capture-MS Homo sapiens
109 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
111 RAP1B 5908
Affinity Capture-MS Homo sapiens
112 NUMB 8650
Affinity Capture-MS Homo sapiens
113 FLOT1 10211
Affinity Capture-MS Homo sapiens
114 SERPINB8 5271
Affinity Capture-MS Homo sapiens
115 S100A8 6279
Affinity Capture-MS Homo sapiens
116 PON2 5445
Affinity Capture-MS Homo sapiens
117 CD207  
Affinity Capture-MS Homo sapiens
118 CREB3  
Two-hybrid Homo sapiens
119 LGALS1 3956
Affinity Capture-MS Homo sapiens
120 RPN1 6184
Proximity Label-MS Homo sapiens
121 PTTG1IP 754
Two-hybrid Homo sapiens
122 VMA21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 GDPD3 79153
Affinity Capture-MS Homo sapiens
124 NRDE2  
Affinity Capture-MS Homo sapiens
125 TGM3 7053
Affinity Capture-MS Homo sapiens
126 SERPINB7 8710
Affinity Capture-MS Homo sapiens
127 ATP6V1H 51606
Affinity Capture-MS Homo sapiens
128 FLOT2 2319
Affinity Capture-MS Homo sapiens
129 RHOT2 89941
Proximity Label-MS Homo sapiens
130 ELOVL5 60481
Proximity Label-MS Homo sapiens
131 TMUB1  
Affinity Capture-MS Homo sapiens
132 SEC61B 10952
Proximity Label-MS Homo sapiens
133 ATP6V1E1 529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 REN  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
135 ZACN  
Affinity Capture-MS Homo sapiens
136 RMDN3 55177
Proximity Label-MS Homo sapiens
137 GPRC5A 9052
Affinity Capture-MS Homo sapiens
138 TMEM185A  
Affinity Capture-MS Homo sapiens
139 ENO1 2023
Affinity Capture-MS Homo sapiens
140 UPK2 7379
Two-hybrid Homo sapiens
141 REEP5 7905
Proximity Label-MS Homo sapiens
142 TGM5  
Affinity Capture-MS Homo sapiens
143 DMKN  
Affinity Capture-MS Homo sapiens
144 SEC63 11231
Proximity Label-MS Homo sapiens
145 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 HSD3B7 80270
Proximity Label-MS Homo sapiens
147 IVL 3713
Affinity Capture-MS Homo sapiens
148 CLINT1 9685
Affinity Capture-MS Homo sapiens
149 RPN2 6185
Proximity Label-MS Homo sapiens
150 CUL3 8452
Affinity Capture-MS Homo sapiens
151 CANX 821
Affinity Capture-MS Homo sapiens
152 CALML5 51806
Affinity Capture-MS Homo sapiens
153 LRRC59 55379
Proximity Label-MS Homo sapiens
154 PANX1 24145
Proximity Label-MS Homo sapiens
155 IL1RN 3557
Affinity Capture-MS Homo sapiens
156 SERPINB13 5275
Affinity Capture-MS Homo sapiens
157 CLTA 1211
Affinity Capture-MS Homo sapiens
158 LAMP1 3916
Proximity Label-MS Homo sapiens
159 ETFB 2109
Affinity Capture-MS Homo sapiens
160 ATP6V1D 51382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 BCAP31 10134
Proximity Label-MS Homo sapiens
162 TMEM199  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 ATP6AP2 10159
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
164 S100A7 6278
Affinity Capture-MS Homo sapiens
165 STIM1 6786
Proximity Label-MS Homo sapiens
166 PDIA3 2923
Affinity Capture-MS Homo sapiens
167 SPECC1 92521
Affinity Capture-MS Homo sapiens
168 FKBP8 23770
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 VAMP3 9341
Affinity Capture-MS Homo sapiens
170 ARF6 382
Proximity Label-MS Homo sapiens
171 HTR2C  
Two-hybrid Homo sapiens
172 CD59 966
Affinity Capture-MS Homo sapiens
173 ATP6V0A4 50617
Affinity Capture-MS Homo sapiens
174 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
175 NXF1 10482
Affinity Capture-RNA Homo sapiens
176 TCIRG1 10312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 PHLDB2 90102
Affinity Capture-MS Homo sapiens
178 ATP6V0E1  
Affinity Capture-MS Homo sapiens
179 MAPK6  
Affinity Capture-MS Homo sapiens
180 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
181 IST1 9798
Affinity Capture-MS Homo sapiens
182 ESYT2 57488
Affinity Capture-MS Homo sapiens
183 PACSIN3 29763
Affinity Capture-MS Homo sapiens
184 ATP2A1 487
Proximity Label-MS Homo sapiens
185 S100A7A 338324
Affinity Capture-MS Homo sapiens
186 GPR45  
Affinity Capture-MS Homo sapiens
187 ATP6V1A 523
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 A4GNT  
Affinity Capture-MS Homo sapiens
189 RAB9A 9367
Proximity Label-MS Homo sapiens
190 LGALS7B 653499
Affinity Capture-MS Homo sapiens
191 RAB11FIP2  
Affinity Capture-MS Homo sapiens
192 WDR75  
Affinity Capture-MS Homo sapiens
193 PMP22  
Two-hybrid Homo sapiens
194 DHFRL1  
Proximity Label-MS Homo sapiens
195 SYNGR2 9144
Affinity Capture-MS Homo sapiens
196 KTN1 3895
Affinity Capture-MS Homo sapiens
197 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 CLGN 1047
Affinity Capture-MS Homo sapiens
199 NKG7  
Two-hybrid Homo sapiens
200 SDR9C7  
Affinity Capture-MS Homo sapiens
201 LMNA 4000
Proximity Label-MS Homo sapiens
202 ATP6AP1 537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
203 SLC14A1  
Affinity Capture-MS Homo sapiens
204 RAB11A 8766
Affinity Capture-MS Homo sapiens
205 CHI3L2 1117
Affinity Capture-MS Homo sapiens
206 STUB1 10273
Affinity Capture-MS Homo sapiens
207 MECR 51102
Co-fractionation Homo sapiens
208 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
209 SLC38A2 54407
Affinity Capture-MS Homo sapiens
210 RNASE7 84659
Affinity Capture-MS Homo sapiens
211 LAMTOR1 55004
Affinity Capture-MS Homo sapiens
212 SERPINA12  
Affinity Capture-MS Homo sapiens
213 JUP 3728
Affinity Capture-MS Homo sapiens
214 PTRF 284119
Affinity Capture-MS Homo sapiens
215 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
216 PDIA4 9601
Proximity Label-MS Homo sapiens
217 WBP11  
Affinity Capture-MS Homo sapiens
218 OSTM1 28962
Affinity Capture-MS Homo sapiens
219 TGM1 7051
Affinity Capture-MS Homo sapiens
220 LAMTOR2 28956
Affinity Capture-MS Homo sapiens
221 GOSR1 9527
Affinity Capture-MS Homo sapiens
222 SLC35F6 54978
Affinity Capture-MS Homo sapiens
223 KLK7 5650
Affinity Capture-MS Homo sapiens
224 MFSD4  
Affinity Capture-MS Homo sapiens
225 ERGIC1 57222
Proximity Label-MS Homo sapiens
226 SERPINB3 6317
Affinity Capture-MS Homo sapiens
227 KIAA1715 80856
Proximity Label-MS Homo sapiens
228 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
229 SFTPC  
Affinity Capture-MS Homo sapiens
230 GOLIM4 27333
Affinity Capture-MS Homo sapiens
231 RABL3 285282
Affinity Capture-MS Homo sapiens
232 WWP2 11060
Affinity Capture-MS Homo sapiens
233 ATP6V0C 527
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
234 ARSF 416
Affinity Capture-MS Homo sapiens
235 ALAD 210
Affinity Capture-MS Homo sapiens
236 SEC62 7095
Proximity Label-MS Homo sapiens
237 GM2A 2760
Affinity Capture-MS Homo sapiens
238 B3GAT1  
Proximity Label-MS Homo sapiens
239 RAB7A 7879
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
240 SEC22A  
Two-hybrid Homo sapiens
241 NCCRP1 342897
Affinity Capture-MS Homo sapiens
242 VAMP5 10791
Two-hybrid Homo sapiens
243 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
244 DNAJC1 64215
Proximity Label-MS Homo sapiens
245 VIPR1  
Affinity Capture-MS Homo sapiens
246 RAB5C 5878
Proximity Label-MS Homo sapiens
247 ECM1 1893
Affinity Capture-MS Homo sapiens
248 LCN2 3934
Affinity Capture-MS Homo sapiens
249 NEU2  
Affinity Capture-MS Homo sapiens
250 CKAP4 10970
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
251 PCDHB3  
Affinity Capture-MS Homo sapiens
252 VIM 7431
Affinity Capture-MS Homo sapiens
253 FKBP2 2286
Co-fractionation Homo sapiens
254 RANGAP1 5905
Affinity Capture-MS Homo sapiens
255 USP20  
Affinity Capture-MS Homo sapiens
256 CTSH 1512
Affinity Capture-MS Homo sapiens
257 DERL1 79139
Proximity Label-MS Homo sapiens
258 DKKL1  
Affinity Capture-MS Homo sapiens
259 FLG2 388698
Affinity Capture-MS Homo sapiens
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Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here