Gene description for ACPP
Gene name acid phosphatase, prostate
Gene symbol ACPP
Other names/aliases 5'-NT
ACP-3
ACP3
Species Homo sapiens
 Database cross references - ACPP
ExoCarta ExoCarta_55
Vesiclepedia VP_55
Entrez Gene 55
HGNC 125
MIM 171790
UniProt P15309  
 ACPP identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ACPP
Molecular Function
    acid phosphatase activity GO:0003993 IDA
    acid phosphatase activity GO:0003993 IMP
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    5'-nucleotidase activity GO:0008253 IDA
    phosphatase activity GO:0016791 IBA
    phosphatase activity GO:0016791 IMP
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    choline binding GO:0033265 IEA
    thiamine phosphate phosphatase activity GO:0042131 ISS
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    lysophosphatidic acid phosphatase activity GO:0052642 IDA
    molecular adaptor activity GO:0060090 EXP
    XMP 5'-nucleosidase activity GO:0106411 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    purine nucleobase metabolic process GO:0006144 IEA
    chromatin remodeling GO:0006338 IEA
    lipid metabolic process GO:0006629 IEA
    thiamine metabolic process GO:0006772 ISS
    nucleotide metabolic process GO:0009117 IEA
    dephosphorylation GO:0016311 IMP
    adenosine metabolic process GO:0046085 IDA
    regulation of sensory perception of pain GO:0051930 IBA
    regulation of sensory perception of pain GO:0051930 IMP
    positive regulation of adenosine receptor signaling pathway GO:0060168 IBA
    positive regulation of adenosine receptor signaling pathway GO:0060168 IMP
Subcellular Localization
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IMP
    nucleus GO:0005634 HDA
    lysosomal membrane GO:0005765 HDA
    multivesicular body GO:0005771 IEA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    vesicle membrane GO:0012506 ISS
    filopodium GO:0030175 IEA
    Golgi cisterna GO:0031985 IEA
    azurophil granule membrane GO:0035577 TAS
    apical part of cell GO:0045177 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ACPP in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
3
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
4
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
5
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ACPP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC103  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MBD4  
Affinity Capture-MS Homo sapiens
3 UBC 7316
Affinity Capture-MS Homo sapiens
4 SEMG2 6407
Affinity Capture-MS Homo sapiens
5 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLAC8L1  
Affinity Capture-MS Homo sapiens
7 ST7  
Affinity Capture-MS Homo sapiens
8 AZGP1 563
Affinity Capture-MS Homo sapiens
9 HSF2  
Affinity Capture-MS Homo sapiens
10 EFNA3  
Affinity Capture-MS Homo sapiens
11 L2HGDH  
Affinity Capture-MS Homo sapiens
12 LRRC10  
Affinity Capture-MS Homo sapiens
13 IL31RA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ACPP 55
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
15 E2F6 1876
Affinity Capture-MS Homo sapiens
16 NUDT3 11165
Affinity Capture-MS Homo sapiens
17 CPLX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PHF11 51131
Affinity Capture-MS Homo sapiens
19 KIF3A 11127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 C18orf21  
Affinity Capture-MS Homo sapiens
21 GAR1 54433
Affinity Capture-MS Homo sapiens
22 FCF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HAT1 8520
Affinity Capture-MS Homo sapiens
24 DDX19B 11269
Affinity Capture-MS Homo sapiens
25 ZNF550  
Affinity Capture-MS Homo sapiens
26 FCRL5  
Affinity Capture-MS Homo sapiens
27 ST6GALNAC6 30815
Affinity Capture-MS Homo sapiens
28 LACC1  
Affinity Capture-MS Homo sapiens
29 RNF115  
Affinity Capture-MS Homo sapiens
30 TPPP 11076
Affinity Capture-MS Homo sapiens
31 MSMB  
Affinity Capture-MS Homo sapiens
32 PEMT  
Affinity Capture-MS Homo sapiens
33 PAG1 55824
Affinity Capture-MS Homo sapiens
34 SARAF  
Affinity Capture-MS Homo sapiens
35 DHH  
Affinity Capture-MS Homo sapiens
36 SSUH2  
Affinity Capture-MS Homo sapiens
37 FBXL4 26235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 MRM1  
Affinity Capture-MS Homo sapiens
39 RHBDD1 84236
Affinity Capture-MS Homo sapiens
40 EFNA4  
Affinity Capture-MS Homo sapiens
41 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
42 CCNC  
Affinity Capture-MS Homo sapiens
43 NT5C1A  
Affinity Capture-MS Homo sapiens
44 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PCP4  
Affinity Capture-MS Homo sapiens
46 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
47 NPPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FTH1 2495
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PLD5  
Affinity Capture-MS Homo sapiens
50 SEMG1 6406
Affinity Capture-MS Homo sapiens
51 ANXA9  
Affinity Capture-MS Homo sapiens
52 FOS 2353
Affinity Capture-MS Homo sapiens
53 KLK3  
Affinity Capture-MS Homo sapiens
54 PRSS1 5644
Affinity Capture-MS Homo sapiens
55 DUSP21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SEH1L 81929
Affinity Capture-MS Homo sapiens
57 SHBG 6462
Two-hybrid Homo sapiens
58 RPS6KB2  
Affinity Capture-MS Homo sapiens
59 SLC37A4 2542
Affinity Capture-MS Homo sapiens
60 MMRN1 22915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 STX17 55014
Affinity Capture-MS Homo sapiens
62 RIPPLY3  
Affinity Capture-MS Homo sapiens
63 PRSS16 10279
Affinity Capture-MS Homo sapiens
64 FAM153B  
Affinity Capture-MS Homo sapiens
65 SLC25A6 293
Affinity Capture-MS Homo sapiens
66 NMB  
Affinity Capture-MS Homo sapiens
67 SH2D3A  
Affinity Capture-MS Homo sapiens
68 CAPZA2 830
Affinity Capture-MS Homo sapiens
69 CDK15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 C1orf35  
Affinity Capture-MS Homo sapiens
71 TREX2  
Affinity Capture-MS Homo sapiens
72 PTDSS1 9791
Affinity Capture-MS Homo sapiens
73 KLHL11  
Affinity Capture-MS Homo sapiens
74 ERCC3  
Affinity Capture-MS Homo sapiens
75 MBNL1 4154
Affinity Capture-MS Homo sapiens
76 KDELR2 11014
Affinity Capture-MS Homo sapiens
77 TEFM  
Affinity Capture-MS Homo sapiens
78 AIRE  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TSPAN6 7105
Affinity Capture-MS Homo sapiens
80 PINK1  
Affinity Capture-MS Homo sapiens
81 GOT1 2805
Affinity Capture-MS Homo sapiens
82 FOXN3 1112
Affinity Capture-MS Homo sapiens
83 OR2A4 79541
Affinity Capture-MS Homo sapiens
84 GABPA 2551
Affinity Capture-MS Homo sapiens
85 CCR1  
Affinity Capture-MS Homo sapiens
86 IFNW1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ACPP is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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