Gene description for MMRN1
Gene name multimerin 1
Gene symbol MMRN1
Other names/aliases ECM
EMILIN4
GPIa*
MMRN
Species Homo sapiens
 Database cross references - MMRN1
ExoCarta ExoCarta_22915
Vesiclepedia VP_22915
Entrez Gene 22915
HGNC 7178
MIM 601456
UniProt Q13201  
 MMRN1 identified in sEVs derived from the following tissue/cell type
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for MMRN1
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 TAS
    blood coagulation GO:0007596 TAS
    negative regulation of vascular endothelial growth factor receptor signaling pathway GO:0030948 IBA
    cell adhesion mediated by integrin GO:0033627 IDA
    negative regulation of wound healing GO:0061045 IDA
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 IBA
    positive regulation of platelet aggregation GO:1901731 IDA
Subcellular Localization
    extracellular region GO:0005576 IMP
    extracellular region GO:0005576 TAS
    platelet alpha granule GO:0031091 IDA
    platelet alpha granule lumen GO:0031093 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    multimerin complex GO:1990972 IPI
 Experiment description of studies that identified MMRN1 in sEVs
1
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MMRN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CEP57  
Affinity Capture-MS Homo sapiens
2 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ACPP 55
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KPRP 448834
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SERPINB4 6318
Affinity Capture-MS Homo sapiens
6 ECM1 1893
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 APOE 348
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CDSN 1041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RNASE7 84659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PDAP1 11333
Cross-Linking-MS (XL-MS) Homo sapiens
11 S100A7 6278
Affinity Capture-MS Homo sapiens
12 SBSN 374897
Affinity Capture-MS Homo sapiens
13 F5 2153
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 CST6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CTSV 1515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CDKN2A 1029
Reconstituted Complex Homo sapiens
18 CKMT1A 548596
Cross-Linking-MS (XL-MS) Homo sapiens
19 AZGP1 563
Affinity Capture-MS Homo sapiens
20 ALOX12B  
Affinity Capture-MS Homo sapiens
21 CTSH 1512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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