Gene description for APOE
Gene name apolipoprotein E
Gene symbol APOE
Other names/aliases AD2
APO-E
LDLCQ5
LPG
Species Homo sapiens
 Database cross references - APOE
ExoCarta ExoCarta_348
Vesiclepedia VP_348
Entrez Gene 348
HGNC 613
MIM 107741
UniProt P02649  
 APOE identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast milk 17641064    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Melanoma cells 22635005    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for APOE
Molecular Function
    amyloid-beta binding GO:0001540 IDA
    amyloid-beta binding GO:0001540 IPI
    signaling receptor binding GO:0005102 IPI
    structural molecule activity GO:0005198 TAS
    lipid transporter activity GO:0005319 IDA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    phospholipid binding GO:0005543 IDA
    heparin binding GO:0008201 IDA
    lipid binding GO:0008289 IDA
    antioxidant activity GO:0016209 IDA
    enzyme binding GO:0019899 IPI
    identical protein binding GO:0042802 IDA
    protein homodimerization activity GO:0042803 IPI
    heparan sulfate proteoglycan binding GO:0043395 IDA
    protein-containing complex binding GO:0044877 IDA
    metal chelating activity GO:0046911 IDA
    protein dimerization activity GO:0046983 IPI
    receptor ligand activity GO:0048018 IDA
    tau protein binding GO:0048156 IPI
    low-density lipoprotein particle receptor binding GO:0050750 IDA
    low-density lipoprotein particle receptor binding GO:0050750 IPI
    low-density lipoprotein particle receptor binding GO:0050750 NAS
    phosphatidylcholine-sterol O-acyltransferase activator activity GO:0060228 IBA
    phosphatidylcholine-sterol O-acyltransferase activator activity GO:0060228 IDA
    very-low-density lipoprotein particle receptor binding GO:0070326 IDA
    very-low-density lipoprotein particle receptor binding GO:0070326 IPI
    lipoprotein particle binding GO:0071813 IEA
    cholesterol transfer activity GO:0120020 IBA
Biological Process
    response to reactive oxygen species GO:0000302 NAS
    negative regulation of endothelial cell proliferation GO:0001937 IDA
    response to dietary excess GO:0002021 IEA
    triglyceride metabolic process GO:0006641 IDA
    triglyceride metabolic process GO:0006641 IMP
    cholesterol catabolic process GO:0006707 IEA
    intracellular calcium ion homeostasis GO:0006874 IEA
    receptor-mediated endocytosis GO:0006898 IDA
    receptor-mediated endocytosis GO:0006898 TAS
    cytoskeleton organization GO:0007010 TAS
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    synaptic transmission, cholinergic GO:0007271 TAS
    long-term memory GO:0007616 IGI
    cholesterol metabolic process GO:0008203 IBA
    cholesterol metabolic process GO:0008203 IDA
    cholesterol metabolic process GO:0008203 IMP
    gene expression GO:0010467 IEA
    negative regulation of platelet activation GO:0010544 IDA
    negative regulation of endothelial cell migration GO:0010596 IMP
    negative regulation of gene expression GO:0010629 ISS
    positive regulation of cholesterol efflux GO:0010875 IDA
    positive regulation of cholesterol efflux GO:0010875 IGI
    lipid transport involved in lipid storage GO:0010877 ISS
    positive regulation of neuron projection development GO:0010976 IDA
    positive regulation of neuron projection development GO:0010976 ISS
    negative regulation of neuron projection development GO:0010977 IDA
    long-chain fatty acid transport GO:0015909 IDA
    protein import GO:0017038 IDA
    virion assembly GO:0019068 IMP
    negative regulation of blood coagulation GO:0030195 IDA
    regulation of axon extension GO:0030516 TAS
    neuron projection development GO:0031175 IDA
    melanosome organization GO:0032438 IMP
    regulation of Cdc42 protein signal transduction GO:0032489 IDA
    positive regulation of low-density lipoprotein particle receptor catabolic process GO:0032805 IDA
    cholesterol efflux GO:0033344 IBA
    cholesterol efflux GO:0033344 IDA
    phospholipid efflux GO:0033700 IBA
    phospholipid efflux GO:0033700 IDA
    very-low-density lipoprotein particle remodeling GO:0034372 IDA
    very-low-density lipoprotein particle remodeling GO:0034372 IGI
    low-density lipoprotein particle remodeling GO:0034374 IEA
    high-density lipoprotein particle remodeling GO:0034375 IGI
    high-density lipoprotein particle assembly GO:0034380 IDA
    chylomicron remnant clearance GO:0034382 IDA
    chylomicron remnant clearance GO:0034382 IMP
    high-density lipoprotein particle clearance GO:0034384 IDA
    very-low-density lipoprotein particle clearance GO:0034447 IDA
    very-low-density lipoprotein particle clearance GO:0034447 IMP
    locomotory exploration behavior GO:0035641 IMP
    nitric oxide-cGMP-mediated signaling GO:0038060 IDA
    lipoprotein biosynthetic process GO:0042158 IDA
    lipoprotein catabolic process GO:0042159 IEA
    vasodilation GO:0042311 IEA
    cholesterol homeostasis GO:0042632 IDA
    cholesterol homeostasis GO:0042632 IGI
    regulation of apoptotic process GO:0042981 IEA
    amyloid precursor protein metabolic process GO:0042982 IDA
    regulation of protein-containing complex assembly GO:0043254 IDA
    negative regulation of MAP kinase activity GO:0043407 IDA
    negative regulation of blood vessel endothelial cell migration GO:0043537 IDA
    reverse cholesterol transport GO:0043691 IDA
    positive regulation by host of viral process GO:0044794 IMP
    regulation of innate immune response GO:0045088 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IDA
    negative regulation of cholesterol biosynthetic process GO:0045541 IDA
    positive regulation of endocytosis GO:0045807 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of lipid biosynthetic process GO:0046889 IDA
    intracellular transport GO:0046907 TAS
    regulation of neuronal synaptic plasticity GO:0048168 TAS
    negative regulation of smooth muscle cell proliferation GO:0048662 ISS
    artery morphogenesis GO:0048844 IEA
    negative regulation of protein secretion GO:0050709 IMP
    negative regulation of inflammatory response GO:0050728 IDA
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 IDA
    positive regulation of membrane protein ectodomain proteolysis GO:0051044 IGI
    negative regulation of lipid biosynthetic process GO:0051055 IDA
    regulation of protein metabolic process GO:0051246 IGI
    negative regulation of protein metabolic process GO:0051248 IGI
    maintenance of location in cell GO:0051651 IEA
    fatty acid homeostasis GO:0055089 IDA
    acylglycerol homeostasis GO:0055090 IBA
    positive regulation of dendritic spine development GO:0060999 IDA
    negative regulation of dendritic spine development GO:0061000 IDA
    regulation of proteasomal protein catabolic process GO:0061136 IMP
    response to caloric restriction GO:0061771 IGI
    triglyceride homeostasis GO:0070328 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    cellular response to lipoprotein particle stimulus GO:0071402 IDA
    triglyceride-rich lipoprotein particle clearance GO:0071830 IMP
    intermediate-density lipoprotein particle clearance GO:0071831 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    regulation of cholesterol metabolic process GO:0090181 IGI
    positive regulation of cholesterol metabolic process GO:0090205 IDA
    negative regulation of triglyceride metabolic process GO:0090209 IEA
    negative regulation of cholesterol efflux GO:0090370 IDA
    AMPA glutamate receptor clustering GO:0097113 IDA
    NMDA glutamate receptor clustering GO:0097114 IDA
    cellular oxidant detoxification GO:0098869 IEA
    positive regulation of lipoprotein transport GO:0140077 IDA
    regulation of amyloid-beta clearance GO:1900221 IDA
    positive regulation of amyloid-beta clearance GO:1900223 ISS
    negative regulation of long-term synaptic potentiation GO:1900272 IDA
    negative regulation of postsynaptic membrane organization GO:1901627 IDA
    positive regulation of presynaptic membrane organization GO:1901631 IDA
    positive regulation of amyloid-beta formation GO:1902004 IDA
    negative regulation of amyloid-beta formation GO:1902430 IDA
    negative regulation of dendritic spine maintenance GO:1902951 IDA
    positive regulation of dendritic spine maintenance GO:1902952 IDA
    regulation of amyloid precursor protein catabolic process GO:1902991 IDA
    positive regulation of phospholipid efflux GO:1902995 IDA
    positive regulation of neurofibrillary tangle assembly GO:1902998 IDA
    negative regulation of phospholipid efflux GO:1902999 IDA
    negative regulation of lipid transport across blood-brain barrier GO:1903001 IDA
    positive regulation of lipid transport across blood-brain barrier GO:1903002 IDA
    regulation of cellular response to very-low-density lipoprotein particle stimulus GO:1905890 IDA
    regulation of amyloid fibril formation GO:1905906 IDA
    negative regulation of amyloid fibril formation GO:1905907 ISS
    positive regulation of amyloid fibril formation GO:1905908 TAS
    positive regulation of CoA-transferase activity GO:1905920 IDA
    regulation of behavioral fear response GO:2000822 IMP
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISS
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 NAS
    cytoplasm GO:0005737 TAS
    early endosome GO:0005769 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 HDA
    dendrite GO:0030425 NAS
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    extracellular matrix GO:0031012 IDA
    chylomicron remnant GO:0034360 IDA
    very-low-density lipoprotein particle GO:0034361 IBA
    very-low-density lipoprotein particle GO:0034361 IDA
    low-density lipoprotein particle GO:0034362 IBA
    low-density lipoprotein particle GO:0034362 IDA
    intermediate-density lipoprotein particle GO:0034363 IDA
    high-density lipoprotein particle GO:0034364 IBA
    high-density lipoprotein particle GO:0034364 IDA
    discoidal high-density lipoprotein particle GO:0034365 TAS
    melanosome GO:0042470 IDA
    chylomicron GO:0042627 IBA
    chylomicron GO:0042627 IDA
    neuronal cell body GO:0043025 NAS
    synaptic cleft GO:0043083 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
    endocytic vesicle lumen GO:0071682 TAS
    blood microparticle GO:0072562 HDA
    multivesicular body, internal vesicle GO:0097487 IDA
    glutamatergic synapse GO:0098978 IDA
    extracellular vesicle GO:1903561 HDA
    extracellular vesicle GO:1903561 IBA
    lipoprotein particle GO:1990777 IDA
 Experiment description of studies that identified APOE in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 48
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
HSC70|CD63|CD86|MHCII
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17641064    
Organism Homo sapiens
Experiment description Exosomes with immune modulatory features are present in human breast milk.
Authors "Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S"
Journal name JIMMU
Publication year 2007
Sample Breast milk
Sample name Breast milk - Mature milk
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.10-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
FACS
9
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
14
Experiment ID 234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
15
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
16
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 200
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|TSG101|Alix|GAPDH
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22635005    
Organism Homo sapiens
Experiment description Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET.
Authors "Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, Hergueta-Redondo M, Williams C, Garcia-Santos G, Ghajar C, Nitadori-Hoshino A, Hoffman C, Badal K, Garcia BA, Callahan MK, Yuan J, Martins VR, Skog J, Kaplan RN, Brady MS, Wolchok JD, Chapman PB, Kang Y, Bromberg J, Lyden D."
Journal name Nat Med
Publication year 2012
Sample Melanoma cells
Sample name B16-F10
SK-MEL-202
SK-MEL035
SK-MEL-265
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
20
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
25
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
27
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
28
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
30
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
31
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
32
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
33
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
34
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
35
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
36
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
37
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
38
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
39
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
40
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for APOE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Co-fractionation Homo sapiens
2 CSTL1  
Affinity Capture-MS Homo sapiens
3 POR 5447
Co-fractionation Homo sapiens
4 CEACAM8 1088
Affinity Capture-MS Homo sapiens
5 SORL1 6653
Reconstituted Complex Homo sapiens
6 TPP1 1200
Co-fractionation Homo sapiens
7 MID1IP1  
Two-hybrid Homo sapiens
8 ALB 213
Affinity Capture-MS Homo sapiens
9 APOE 348
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 CSNK2A1 1457
Biochemical Activity Homo sapiens
11 LOXL4 84171
Two-hybrid Homo sapiens
12 PDHB 5162
Co-fractionation Homo sapiens
13 APP 351
Protein-peptide Homo sapiens
14 HSPA5 3309
Co-fractionation Homo sapiens
15 PTGES2 80142
Co-fractionation Homo sapiens
16 LRPPRC 10128
Co-fractionation Homo sapiens
17 LRP1 4035
Affinity Capture-Western Homo sapiens
18 IFIT5 24138
Two-hybrid Homo sapiens
19 RHEB 6009
Two-hybrid Homo sapiens
20 NDUFS3 4722
Co-fractionation Homo sapiens
21 OGDH 4967
Co-fractionation Homo sapiens
22 PRDX3 10935
Co-fractionation Homo sapiens
23 NDUFB8 4714
Co-fractionation Homo sapiens
24 CYB5R1 51706
Co-fractionation Homo sapiens
25 IFIT3 3437
Two-hybrid Homo sapiens
26 LONP1 9361
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
27 PRDX2 7001
Two-hybrid Homo sapiens
28 Set 56086
Reconstituted Complex Mus musculus
29 ATP5O 539
Co-fractionation Homo sapiens
30 NDUFV1 4723
Co-fractionation Homo sapiens
31 PHB 5245
Co-fractionation Homo sapiens
32 NDUFS1 4719
Co-fractionation Homo sapiens
33 TMEM59 9528
Affinity Capture-MS Homo sapiens
34 APOC2 344
Affinity Capture-MS Homo sapiens
35 SFXN1 94081
Co-fractionation Homo sapiens
36 HSPA8 3312
Affinity Capture-MS Homo sapiens
37 NNT 23530
Co-fractionation Homo sapiens
38 FARSA 2193
Two-hybrid Homo sapiens
39 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 ATP5A1 498
Co-fractionation Homo sapiens
41 P4HA1 5033
Co-fractionation Homo sapiens
42 RNF32  
Two-hybrid Homo sapiens
43 RPL4 6124
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
44 MMP3 4314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 MMP10 4319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NDUFA8 4702
Co-fractionation Homo sapiens
47 A2M 2
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
48 TUFM 7284
Co-fractionation Homo sapiens
49 MAPT  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
50 VCP 7415
Co-fractionation Homo sapiens
51 APOA2 336
Cross-Linking-MS (XL-MS) Homo sapiens
52 HMOX2 3163
Co-fractionation Homo sapiens
53 RPS7 6201
Affinity Capture-MS Homo sapiens
54 EEF2 1938
Co-fractionation Homo sapiens
55 CYC1 1537
Co-fractionation Homo sapiens
56 UQCRC1 7384
Co-fractionation Homo sapiens
57 ACTB 60
Co-fractionation Homo sapiens
58 UQCRH 7388
Co-fractionation Homo sapiens
59 AGPS 8540
Co-fractionation Homo sapiens
60 KLK10 5655
Affinity Capture-MS Homo sapiens
61 ACTG1 71
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
62 ATP5B 506
Co-fractionation Homo sapiens
63 HTRA1 5654
Two-hybrid Homo sapiens
64 ARFGAP1 55738
Two-hybrid Homo sapiens
65 ST13 6767
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
66 LRP8 7804
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
67 C9 735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 SLC25A24 29957
Co-fractionation Homo sapiens
69 IQSEC1 9922
Two-hybrid Homo sapiens
70 PSEN1 5663
Two-hybrid Homo sapiens
71 HADHA 3030
Co-fractionation Homo sapiens
72 CANX 821
Co-fractionation Homo sapiens
73 RAB7A 7879
Co-fractionation Homo sapiens
74 EMC8 10328
Co-fractionation Homo sapiens
75 LRRC59 55379
Co-fractionation Homo sapiens
76 LETM1 3954
Co-fractionation Homo sapiens
77 NOS3  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
78 UQCRC2 7385
Co-fractionation Homo sapiens
79 NPTN 27020
Affinity Capture-MS Homo sapiens
80 CTNNB1 1499
Affinity Capture-MS Homo sapiens
81 GCDH 2639
Two-hybrid Homo sapiens
82 FOXG1  
Two-hybrid Homo sapiens
83 YWHAZ 7534
Affinity Capture-MS Homo sapiens
84 C16orf92  
Affinity Capture-MS Homo sapiens
85 FBXL12 54850
Two-hybrid Homo sapiens
86 CYP2C18  
Two-hybrid Homo sapiens
87 PDCD4 27250
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
88 PLTP 5360
Affinity Capture-Western Homo sapiens
89 TPM1 7168
Affinity Capture-MS Homo sapiens
90 MICB  
Affinity Capture-MS Homo sapiens
91 ZNF558  
Two-hybrid Homo sapiens
92 NEFM 4741
Reconstituted Complex Homo sapiens
93 EMC2 9694
Co-fractionation Homo sapiens
94 ATP6V1A 523
Co-fractionation Homo sapiens
95 RPS14 6208
Affinity Capture-MS Homo sapiens
96 FXYD7  
Two-hybrid Homo sapiens
97 RCN2 5955
Co-fractionation Homo sapiens
98 VDAC1 7416
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 CLTC 1213
Affinity Capture-MS Homo sapiens
100 AKT1 207
Biochemical Activity Homo sapiens
101 MMRN1 22915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 TFRC 7037
Co-fractionation Homo sapiens
103 SCCPDH 51097
Co-fractionation Homo sapiens
104 TPM3 7170
Affinity Capture-MS Homo sapiens
105 CACNA2D3  
Affinity Capture-MS Homo sapiens
106 CYP2C8  
Two-hybrid Homo sapiens
107 SNCA 6622
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
108 PHB2 11331
Co-fractionation Homo sapiens
109 PLEKHA6 22874
Two-hybrid Homo sapiens
110 RRBP1 6238
Affinity Capture-MS Homo sapiens
111 RPL6 6128
Affinity Capture-MS Homo sapiens
112 PCMT1 5110
Two-hybrid Homo sapiens
113 IFNA8  
Affinity Capture-MS Homo sapiens
114 SAA1 6288
Cross-Linking-MS (XL-MS) Homo sapiens
115 CDC37 11140
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
116 TYRO3 7301
Two-hybrid Homo sapiens
117 LCAT 3931
Biochemical Activity Homo sapiens
118 NDUFS7 374291
Co-fractionation Homo sapiens
119 ELAVL1 1994
Two-hybrid Homo sapiens
120 EPN2 22905
Two-hybrid Homo sapiens
121 ATP2A2 488
Co-fractionation Homo sapiens
122 ANKH 56172
Two-hybrid Homo sapiens
123 PRAM1  
Two-hybrid Homo sapiens
124 RPS26 6231
Affinity Capture-MS Homo sapiens
125 RPS28 6234
Affinity Capture-MS Homo sapiens
126 NIPSNAP1 8508
Co-fractionation Homo sapiens
127 NDUFS8 4728
Co-fractionation Homo sapiens
128 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 PCBP1 5093
Affinity Capture-MS Homo sapiens
130 C19orf52  
Two-hybrid Homo sapiens
131 Lck 16818
Affinity Capture-MS Mus musculus
132 LRP2 4036
Reconstituted Complex Homo sapiens
133 CYB5R3 1727
Co-fractionation Homo sapiens
134 RPL8 6132
Affinity Capture-MS Homo sapiens
135 ATP5C1 509
Co-fractionation Homo sapiens
136 HADHB 3032
Co-fractionation Homo sapiens
137 VDAC3 7419
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 MAST1  
Two-hybrid Homo sapiens
139 BCKDHA 593
Co-fractionation Homo sapiens
140 ECSIT 51295
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which APOE is involved
PathwayEvidenceSource
Amyloid fiber formation TAS Reactome
Binding and Uptake of Ligands by Scavenger Receptors TAS Reactome
Binding and Uptake of Ligands by Scavenger Receptors IEA Reactome
Chylomicron assembly TAS Reactome
Chylomicron clearance TAS Reactome
Chylomicron clearance IEA Reactome
Chylomicron remodeling TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
HDL remodeling TAS Reactome
Metabolism TAS Reactome
Metabolism IEA Reactome
Metabolism of fat-soluble vitamins TAS Reactome
Metabolism of fat-soluble vitamins IEA Reactome
Metabolism of proteins TAS Reactome
Metabolism of vitamins and cofactors TAS Reactome
Metabolism of vitamins and cofactors IEA Reactome
NR1H2 and NR1H3-mediated signaling TAS Reactome
NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
Plasma lipoprotein assembly TAS Reactome
Plasma lipoprotein assembly, remodeling, and clearance TAS Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance TAS Reactome
Plasma lipoprotein clearance IEA Reactome
Plasma lipoprotein remodeling TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein phosphorylation TAS Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) TAS Reactome
Retinoid metabolism and transport TAS Reactome
Retinoid metabolism and transport IEA Reactome
RNA Polymerase II Transcription TAS Reactome
Scavenging by Class A Receptors TAS Reactome
Scavenging by Class A Receptors IEA Reactome
Sensory Perception TAS Reactome
Sensory Perception IEA Reactome
Signal Transduction TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Transcriptional regulation by the AP-2 (TFAP2) family of transcription factors TAS Reactome
Transport of small molecules TAS Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport TAS Reactome
Vesicle-mediated transport IEA Reactome
Visual phototransduction TAS Reactome
Visual phototransduction IEA Reactome





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