Gene description for IFIT5
Gene name interferon-induced protein with tetratricopeptide repeats 5
Gene symbol IFIT5
Other names/aliases ISG58
P58
RI58
Species Homo sapiens
 Database cross references - IFIT5
ExoCarta ExoCarta_24138
Vesiclepedia VP_24138
Entrez Gene 24138
HGNC 13328
MIM 616135
UniProt Q13325  
 IFIT5 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for IFIT5
Molecular Function
    tRNA binding GO:0000049 IDA
    RNA cap binding GO:0000339 IDA
    double-stranded DNA binding GO:0003690 IDA
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IDA
    single-stranded RNA binding GO:0003727 IDA
    protein binding GO:0005515 IPI
    poly(U) RNA binding GO:0008266 IDA
Biological Process
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    negative regulation of viral genome replication GO:0045071 IDA
    innate immune response GO:0045087 IEA
    defense response to virus GO:0051607 IBA
    defense response to virus GO:0051607 IMP
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    IkappaB kinase complex GO:0008385 IMP
    actin cytoskeleton GO:0015629 IDA
    ruffle membrane GO:0032587 IDA
    apical part of cell GO:0045177 IDA
 Experiment description of studies that identified IFIT5 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IFIT5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Two-hybrid Homo sapiens
2 RPL13 6137
Affinity Capture-MS Homo sapiens
3 PHLPP1  
Proximity Label-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 TSFM 10102
Co-fractionation Homo sapiens
7 ALDH9A1 223
Co-fractionation Homo sapiens
8 RAD23A 5886
Two-hybrid Homo sapiens
9 KCTD6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ATG5 9474
Proximity Label-MS Homo sapiens
11 ABCB9  
Cross-Linking-MS (XL-MS) Homo sapiens
12 LIN28A  
Affinity Capture-MS Homo sapiens
13 SRPK2 6733
Biochemical Activity Homo sapiens
14 SSB 6741
Affinity Capture-MS Homo sapiens
15 LONP1 9361
Two-hybrid Homo sapiens
16 RPA2 6118
Proximity Label-MS Homo sapiens
17 MEX3A  
Affinity Capture-RNA Homo sapiens
18 CDC37 11140
Two-hybrid Homo sapiens
19 RNF41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RNF32  
Two-hybrid Homo sapiens
21 APOE 348
Two-hybrid Homo sapiens
22 MAGEL2  
Proximity Label-MS Homo sapiens
23 RPL3 6122
Affinity Capture-MS Homo sapiens
24 EGFR 1956
Affinity Capture-MS Homo sapiens
25 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 ABCB1 5243
Cross-Linking-MS (XL-MS) Homo sapiens
28 MAP3K7  
Co-fractionation Homo sapiens
29 A2M 2
Two-hybrid Homo sapiens
30 RECQL 5965
Co-fractionation Homo sapiens
31 ABCB5  
Cross-Linking-MS (XL-MS) Homo sapiens
32 IFIT1 3434
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 RPA3 6119
Proximity Label-MS Homo sapiens
34 CRKL 1399
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 H19 283120
Protein-RNA Homo sapiens
36 ABCB4 5244
Cross-Linking-MS (XL-MS) Homo sapiens
37 IFIT3 3437
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
38 DMD 1756
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PRDX2 7001
Two-hybrid Homo sapiens
40 MAST1  
Two-hybrid Homo sapiens
41 PRKRA 8575
Affinity Capture-MS Homo sapiens
42 RBMS2 5939
Affinity Capture-MS Homo sapiens
43 SRPK1 6732
Biochemical Activity Homo sapiens
44 USP33  
Affinity Capture-MS Homo sapiens
45 ECSIT 51295
Two-hybrid Homo sapiens
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