Gene description for PSEN2
Gene name presenilin 2
Gene symbol PSEN2
Other names/aliases AD3L
AD4
CMD1V
PS2
STM2
Species Homo sapiens
 Database cross references - PSEN2
ExoCarta ExoCarta_5664
Vesiclepedia VP_5664
Entrez Gene 5664
HGNC 9509
MIM 600759
UniProt P49810  
 PSEN2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PSEN2
Molecular Function
    endopeptidase activity GO:0004175 IBA
    protein binding GO:0005515 IPI
    aspartic-type endopeptidase activity GO:0004190 IEA
Biological Process
    lung alveolus development GO:0048286 IEA
    endoplasmic reticulum calcium ion homeostasis GO:0032469 IEA
    Notch signaling pathway GO:0007219 TAS
    positive regulation of catalytic activity GO:0043085 IDA
    negative regulation of protein binding GO:0032091 IEA
    positive regulation of coagulation GO:0050820 IEA
    negative regulation of protein complex assembly GO:0031333 IEA
    embryonic limb morphogenesis GO:0030326 IEA
    cell fate specification GO:0001708 IEA
    axon guidance GO:0007411 TAS
    response to hypoxia GO:0001666 IEA
    protein transport GO:0015031 IEA
    myeloid leukocyte differentiation GO:0002573 IEA
    memory GO:0007613 IEA
    positive regulation of apoptotic process GO:0043065 TAS
    negative regulation of protein phosphorylation GO:0001933 IEA
    protein processing GO:0016485 IDA
    negative regulation of apoptotic process GO:0043066 IBA
    thymus development GO:0048538 IEA
    calcium ion transport GO:0006816 IBA
    T cell activation involved in immune response GO:0002286 IEA
    beta-amyloid metabolic process GO:0050435 IBA
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    T cell receptor signaling pathway GO:0050852 IEA
    apoptotic signaling pathway GO:0097190 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    Notch receptor processing GO:0007220 TAS
    brain morphogenesis GO:0048854 IEA
    somitogenesis GO:0001756 IEA
    regulation of synaptic plasticity GO:0048167 IEA
    membrane protein intracellular domain proteolysis GO:0031293 TAS
    amyloid precursor protein catabolic process GO:0042987 TAS
    negative regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902042 IEA
    regulation of epidermal growth factor-activated receptor activity GO:0007176 IEA
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 IEA
    dorsal/ventral neural tube patterning GO:0021904 IEA
    hair follicle development GO:0001942 IEA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    cardiac muscle contraction GO:0060048 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    forebrain development GO:0030900 IEA
    intracellular signal transduction GO:0035556 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    growth cone GO:0030426 IBA
    protein complex GO:0043234 IDA
    cell cortex GO:0005938 IBA
    cytosol GO:0005829 IEA
    ciliary basal body GO:0036064 IEA
    dendritic shaft GO:0043198 IBA
    perinuclear region of cytoplasm GO:0048471 IBA
    integral component of plasma membrane GO:0005887 IDA
    membrane GO:0016020 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Z disc GO:0030018 IBA
    lysosomal membrane GO:0005765 IBA
    centrosome GO:0005813 IDA
    kinetochore GO:0000776 IDA
    ciliary rootlet GO:0035253 IBA
    membrane raft GO:0045121 IBA
    nucleus GO:0005634 IBA
    mitochondrial inner membrane GO:0005743 IBA
    plasma membrane GO:0005886 TAS
    neuromuscular junction GO:0031594 IBA
    nuclear inner membrane GO:0005637 IDA
    Golgi membrane GO:0000139 IEA
    endoplasmic reticulum GO:0005783 IDA
    apical plasma membrane GO:0016324 IBA
    axon GO:0030424 IBA
    cell surface GO:0009986 IBA
    neuronal cell body GO:0043025 IBA
 Experiment description of studies that identified PSEN2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PSEN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APBA2 321
Affinity Capture-Western Homo sapiens
2 SRI 6717
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 KCNIP4 80333
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 NCSTN 23385
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 APH1A 51107
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
6 APBA1  
Affinity Capture-Western Homo sapiens
7 UBQLN1 29979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 CSEN  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 CAPN1 823
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 BCL2L1 598
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
11 PSEN1 5663
Affinity Capture-Western Homo sapiens
12 FLNB 2317
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
13 APBA3  
Affinity Capture-Western Homo sapiens
14 FHL2 2274
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
15 GFAP 2670
Two-hybrid Homo sapiens
16 CIB1 10519
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
17 CTNND2 1501
Invivo Homo sapiens
18 DOCK3  
Invitro Homo sapiens
Two-hybrid Homo sapiens
19 METTL2B 55798
Two-hybrid Homo sapiens
20 PSENEN  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
21 ICAM5 7087
Reconstituted Complex Homo sapiens
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