Gene description for GCDH
Gene name glutaryl-CoA dehydrogenase
Gene symbol GCDH
Other names/aliases ACAD5
GCD
Species Homo sapiens
 Database cross references - GCDH
ExoCarta ExoCarta_2639
Vesiclepedia VP_2639
Entrez Gene 2639
HGNC 4189
MIM 608801
UniProt Q92947  
 GCDH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GCDH
Molecular Function
    fatty-acyl-CoA binding GO:0000062 IBA
    glutaryl-CoA dehydrogenase activity GO:0004361 IBA
    glutaryl-CoA dehydrogenase activity GO:0004361 IDA
    flavin adenine dinucleotide binding GO:0050660 IBA
Biological Process
    tryptophan metabolic process GO:0006568 IEA
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 IBA
    fatty acid beta-oxidation using acyl-CoA dehydrogenase GO:0033539 IDA
    fatty-acyl-CoA biosynthetic process GO:0046949 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified GCDH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GCDH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Two-hybrid Homo sapiens
2 MTG2  
Proximity Label-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 KLHL8  
Affinity Capture-MS Homo sapiens
5 C17orf80 55028
Proximity Label-MS Homo sapiens
6 PMPCB 9512
Proximity Label-MS Homo sapiens
7 TACO1  
Proximity Label-MS Homo sapiens
8 NAAA  
Affinity Capture-MS Homo sapiens
9 TSFM 10102
Proximity Label-MS Homo sapiens
10 FAF2 23197
Affinity Capture-MS Homo sapiens
11 VWA8 23078
Affinity Capture-MS Homo sapiens
12 PSEN1 5663
Two-hybrid Homo sapiens
13 MRRF  
Proximity Label-MS Homo sapiens
14 TBRG4 9238
Proximity Label-MS Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 MTIF2 4528
Proximity Label-MS Homo sapiens
17 TRIM33 51592
Affinity Capture-MS Homo sapiens
18 MDH2 4191
Proximity Label-MS Homo sapiens
19 NOS3  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 ADRB2  
Affinity Capture-MS Homo sapiens
21 TRUB2  
Proximity Label-MS Homo sapiens
22 CDC37 11140
Two-hybrid Homo sapiens
23 BRD8 10902
Affinity Capture-MS Homo sapiens
24 ISCA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PHIP 55023
Affinity Capture-MS Homo sapiens
26 Ccdc12  
Affinity Capture-MS Mus musculus
27 APOE 348
Two-hybrid Homo sapiens
28 RNF32  
Two-hybrid Homo sapiens
29 MRPS26 64949
Proximity Label-MS Homo sapiens
30 COX8A  
Proximity Label-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 FBXO6 26270
Affinity Capture-MS Homo sapiens
33 XPNPEP3 63929
Affinity Capture-MS Homo sapiens
34 TAF1  
Affinity Capture-MS Homo sapiens
35 C21orf33  
Proximity Label-MS Homo sapiens
36 MTRF1  
Proximity Label-MS Homo sapiens
37 A2M 2
Two-hybrid Homo sapiens
38 SSBP1 6742
Proximity Label-MS Homo sapiens
39 LRPPRC 10128
Proximity Label-MS Homo sapiens
40 C1QBP 708
Proximity Label-MS Homo sapiens
41 MTRF1L  
Proximity Label-MS Homo sapiens
42 GFM1 85476
Proximity Label-MS Homo sapiens
43 TUBA1C 84790
Affinity Capture-MS Homo sapiens
44 MYC  
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 PMPCA 23203
Proximity Label-MS Homo sapiens
47 AARS2  
Proximity Label-MS Homo sapiens
48 MARS2 92935
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 SPRTN  
Affinity Capture-MS Homo sapiens
50 EXOC6 54536
Two-hybrid Homo sapiens
51 BRD1 23774
Affinity Capture-MS Homo sapiens
52 ACAD9 28976
Proximity Label-MS Homo sapiens
53 PDHA1 5160
Proximity Label-MS Homo sapiens
54 HINT2 84681
Proximity Label-MS Homo sapiens
55 TRIM66  
Affinity Capture-MS Homo sapiens
56 C9orf72  
Affinity Capture-MS Homo sapiens
57 SERBP1 26135
Affinity Capture-MS Homo sapiens
58 METTL17  
Proximity Label-MS Homo sapiens
59 LONP1 9361
Proximity Label-MS Homo sapiens
60 AUH 549
Proximity Label-MS Homo sapiens
61 MAST1  
Two-hybrid Homo sapiens
62 ECSIT 51295
Two-hybrid Homo sapiens
63 SP100 6672
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GCDH is involved
PathwayEvidenceSource
Lysine catabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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