Gene description for SP100
Gene name SP100 nuclear antigen
Gene symbol SP100
Other names/aliases lysp100b
Species Homo sapiens
 Database cross references - SP100
ExoCarta ExoCarta_6672
Vesiclepedia VP_6672
Entrez Gene 6672
HGNC 11206
MIM 604585
UniProt P23497  
 SP100 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SP100
Molecular Function
    kinase binding GO:0019900 IPI
    transcription factor binding GO:0008134 IPI
    DNA binding GO:0003677 IEA
    protein domain specific binding GO:0019904 IPI
    protein homodimerization activity GO:0042803 IPI
    transcription coactivator activity GO:0003713 IDA
    identical protein binding GO:0042802 IPI
    transcription corepressor activity GO:0003714 IDA
    chromo shadow domain binding GO:0070087 IPI
    chromatin binding GO:0003682 IBA
    protein binding GO:0005515 IPI
Biological Process
    response to interferon-gamma GO:0034341 IMP
    negative regulation of viral transcription GO:0032897 IDA
    negative regulation of protein export from nucleus GO:0046826 IMP
    regulation of angiogenesis GO:0045765 IMP
    response to cytokine GO:0034097 IDA
    cellular protein metabolic process GO:0044267 TAS
    positive regulation of sequence-specific DNA binding transcription factor activity GO:0051091 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    negative regulation of cellular component movement GO:0051271 IMP
    viral process GO:0016032 IEA
    response to type I interferon GO:0034340 IMP
    response to retinoic acid GO:0032526 IDA
    protein sumoylation GO:0016925 TAS
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    negative regulation of nucleic acid-templated transcription GO:1903507 IDA
    chromatin remodeling GO:0006338 IBA
    post-translational protein modification GO:0043687 TAS
    interferon-gamma-mediated signaling pathway GO:0060333 TAS
    regulation of Fas signaling pathway GO:1902044 IMP
    retinoic acid receptor signaling pathway GO:0048384 IC
    transcription, DNA-templated GO:0006351 IEA
    negative regulation of DNA binding GO:0043392 IDA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator GO:0006978 IDA
    negative regulation of endothelial cell migration GO:0010596 IMP
    type I interferon signaling pathway GO:0060337 IC
    negative regulation of sequence-specific DNA binding transcription factor activity GO:0043433 IDA
    telomere maintenance GO:0000723 IMP
    regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902041 IMP
    cytokine-mediated signaling pathway GO:0019221 TAS
Subcellular Localization
    PML body GO:0016605 IDA
    nucleoplasm GO:0005654 TAS
    Mre11 complex GO:0030870 IDA
    nuclear periphery GO:0034399 IDA
    nucleolus GO:0005730 IMP
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SP100 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SP100
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIPK2 28996
Affinity Capture-Western Homo sapiens
2 CBX3 11335
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 SNIP1  
Affinity Capture-MS Homo sapiens
4 CBX5 23468
Affinity Capture-MS Homo sapiens
5 SUMO1 7341
Two-hybrid Homo sapiens
6 ETS1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SP100 is involved
PathwayEvidenceSource
Interferon gamma signaling TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here