Gene description for CRTAP
Gene name cartilage associated protein
Gene symbol CRTAP
Other names/aliases CASP
LEPREL3
OI7
P3H5
Species Homo sapiens
 Database cross references - CRTAP
ExoCarta ExoCarta_10491
Vesiclepedia VP_10491
Entrez Gene 10491
HGNC 2379
MIM 605497
UniProt O75718  
 CRTAP identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CRTAP
Molecular Function
    protein binding GO:0005515 IPI
    protein binding GO:0005515 ISS
    collagen binding GO:0005518 IBA
Biological Process
    spermatogenesis GO:0007283 IEA
    collagen fibril organization GO:0030199 IBA
    protein stabilization GO:0050821 IMP
    chaperone-mediated protein folding GO:0061077 ISS
    negative regulation of post-translational protein modification GO:1901874 IMP
Subcellular Localization
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    protein-containing complex GO:0032991 ISS
 Experiment description of studies that identified CRTAP in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
4
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CRTAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 P3H1 64175
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 SNRPA 6626
Affinity Capture-MS Homo sapiens
4 ACTR2 10097
Co-fractionation Homo sapiens
5 NUDT21 11051
Affinity Capture-MS Homo sapiens
6 GPN1  
Co-fractionation Homo sapiens
7 MGARP  
Co-fractionation Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 TOM1L1 10040
Co-fractionation Homo sapiens
10 ZNHIT1  
Affinity Capture-MS Homo sapiens
11 C1QTNF9B  
Affinity Capture-MS Homo sapiens
12 AHDC1  
Affinity Capture-MS Homo sapiens
13 CBFA2T3  
Affinity Capture-MS Homo sapiens
14 FAM20C 56975
Affinity Capture-MS Homo sapiens
15 TMEM87A 25963
Affinity Capture-MS Homo sapiens
16 CPSF6 11052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 KIF14 9928
Affinity Capture-MS Homo sapiens
18 FAM163A  
Affinity Capture-MS Homo sapiens
19 CSTF2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 ADAD2  
Affinity Capture-MS Homo sapiens
21 ARMC9 80210
Co-fractionation Homo sapiens
22 PRELID1  
Affinity Capture-MS Homo sapiens
23 C1orf106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PACSIN2 11252
Co-fractionation Homo sapiens
25 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SF3B4 10262
Affinity Capture-MS Homo sapiens
27 EMID1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 AJUBA  
Affinity Capture-MS Homo sapiens
29 PRG2  
Affinity Capture-MS Homo sapiens
30 HRG 3273
Affinity Capture-MS Homo sapiens
31 RAD18  
Affinity Capture-MS Homo sapiens
32 SURF2  
Co-fractionation Homo sapiens
33 GEMIN2 8487
Affinity Capture-MS Homo sapiens
34 VHL  
Affinity Capture-MS Homo sapiens
35 Sf3a1 67465
Affinity Capture-MS Mus musculus
36 PARK2  
Affinity Capture-MS Homo sapiens
37 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SMR3B  
Affinity Capture-MS Homo sapiens
39 SNRPF 6636
Affinity Capture-MS Homo sapiens
40 SH3GL1 6455
Co-fractionation Homo sapiens
41 ZNF397  
Affinity Capture-MS Homo sapiens
42 IKZF4  
Affinity Capture-MS Homo sapiens
43 COL10A1  
Affinity Capture-MS Homo sapiens
44 VPS26A 9559
Affinity Capture-MS Homo sapiens
45 Srsf1 110809
Affinity Capture-MS Mus musculus
46 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 MBIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 WDR48 57599
Affinity Capture-MS Homo sapiens
49 TSC22D4 81628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 IDS  
Affinity Capture-MS Homo sapiens
51 PRC1 9055
Affinity Capture-MS Homo sapiens
52 VPS37D 155382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 OGT 8473
Reconstituted Complex Homo sapiens
54 RNPS1 10921
Affinity Capture-MS Homo sapiens
55 COL8A2 1296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 COLQ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MIA3 375056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 FIP1L1 81608
Affinity Capture-MS Homo sapiens
59 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 COL8A1 1295
Affinity Capture-MS Homo sapiens
61 HK1 3098
Co-fractionation Homo sapiens
62 POLR2B 5431
Co-fractionation Homo sapiens
63 GAS2L1 10634
Affinity Capture-MS Homo sapiens
64 CIT 11113
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 SH3GLB2 56904
Co-fractionation Homo sapiens
67 RIPPLY3  
Affinity Capture-MS Homo sapiens
68 ALX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SP100 6672
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which CRTAP is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes TAS Reactome
Collagen formation TAS Reactome
Extracellular matrix organization TAS Reactome





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