Gene description for RNPS1
Gene name RNA binding protein S1, serine-rich domain
Gene symbol RNPS1
Other names/aliases E5.1
Species Homo sapiens
 Database cross references - RNPS1
ExoCarta ExoCarta_10921
Entrez Gene 10921
HGNC 10080
MIM 606447
UniProt Q15287  
 RNPS1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for RNPS1
Molecular Function
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    mRNA 3'-UTR binding GO:0003730 IDA
    RNA binding GO:0003723 IDA
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IMP
    RNA splicing GO:0008380 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA export from nucleus GO:0006406 TAS
    mRNA 3'-end processing GO:0031124 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    gene expression GO:0010467 TAS
    transcription, DNA-templated GO:0006351 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    positive regulation of apoptotic process GO:0043065 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    ASAP complex GO:0061574 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    nuclear speck GO:0016607 IEA
    exon-exon junction complex GO:0035145 TAS
 Experiment description of studies that identified RNPS1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RNPS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC2L2  
Invivo Homo sapiens
2 SNRPA 6626
Affinity Capture-MS Homo sapiens
3 NUDT21 11051
Affinity Capture-MS Homo sapiens
4 RBM8A 9939
Reconstituted Complex Homo sapiens
5 AP2B1 163
Affinity Capture-MS Homo sapiens
6 USP7 7874
Affinity Capture-MS Homo sapiens
7 CSNK2B 1460
Affinity Capture-MS Homo sapiens
8 PRPF4B 8899
Affinity Capture-MS Homo sapiens
9 SREK1 140890
Affinity Capture-MS Homo sapiens
10 DVL2 1856
Two-hybrid Homo sapiens
11 LUC7L  
Affinity Capture-MS Homo sapiens
12 C14orf102  
Affinity Capture-MS Homo sapiens
13 RBM25 58517
Affinity Capture-MS Homo sapiens
14 SRPK2 6733
Affinity Capture-MS Homo sapiens
15 STRAP 11171
Affinity Capture-MS Homo sapiens
16 PNN 5411
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 SRSF2 6427
Affinity Capture-MS Homo sapiens
18 PRPF6 24148
Affinity Capture-MS Homo sapiens
19 SRSF1 6426
Affinity Capture-MS Homo sapiens
20 SF3B1 23451
Affinity Capture-MS Homo sapiens
21 WDR33 55339
Affinity Capture-MS Homo sapiens
22 SRRT 51593
Affinity Capture-MS Homo sapiens
23 PRPF8 10594
Affinity Capture-MS Homo sapiens
24 THRAP3 9967
Affinity Capture-MS Homo sapiens
25 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
26 PRPF3  
Affinity Capture-MS Homo sapiens
27 PSME3 10197
Affinity Capture-MS Homo sapiens
28 DDX20  
Affinity Capture-MS Homo sapiens
29 CPSF2 53981
Affinity Capture-MS Homo sapiens
30 DDX41  
Affinity Capture-MS Homo sapiens
31 PUF60 22827
Affinity Capture-MS Homo sapiens
32 PRPF40A 55660
Affinity Capture-MS Homo sapiens
33 DDX23 9416
Affinity Capture-MS Homo sapiens
34 SRRM1  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
35 SRSF6 6431
Two-hybrid Homo sapiens
36 C19orf29  
Affinity Capture-MS Homo sapiens
37 EFTUD2 9343
Affinity Capture-MS Homo sapiens
38 RANBP9 10048
Affinity Capture-MS Homo sapiens
39 SNRNP200 23020
Affinity Capture-MS Homo sapiens
40 GEMIN4 50628
Affinity Capture-MS Homo sapiens
41 CROP  
Affinity Capture-MS Homo sapiens
42 SNRNP70 6625
Affinity Capture-MS Homo sapiens
43 SF3B3 23450
Affinity Capture-MS Homo sapiens
44 DHX38  
Affinity Capture-MS Homo sapiens
45 GID8 54994
Affinity Capture-MS Homo sapiens
46 CDK11B 984
Affinity Capture-MS Homo sapiens
47 SRSF7 6432
Affinity Capture-MS Homo sapiens
48 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
49 U2AF2 11338
Affinity Capture-MS Homo sapiens
50 SFRS8  
Affinity Capture-MS Homo sapiens
51 SART3 9733
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
52 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
53 GEMIN2 8487
Affinity Capture-MS Homo sapiens
54 SRRM2 23524
Affinity Capture-MS Homo sapiens
55 KHK 3795
Affinity Capture-MS Homo sapiens
56 RARS 5917
Affinity Capture-MS Homo sapiens
57 MKLN1  
Affinity Capture-MS Homo sapiens
58 CPSF6 11052
Affinity Capture-MS Homo sapiens
59 LUC7L2  
Affinity Capture-MS Homo sapiens
60 SRPK1 6732
Affinity Capture-MS Homo sapiens
61 TRA2B 6434
Affinity Capture-MS Homo sapiens
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