Gene description for IK
Gene name IK cytokine, down-regulator of HLA II
Gene symbol IK
Other names/aliases CSA2
RED
Species Homo sapiens
 Database cross references - IK
ExoCarta ExoCarta_3550
Vesiclepedia VP_3550
Entrez Gene 3550
HGNC 5958
MIM 600549
UniProt Q13123  
 IK identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IK
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    mitotic cell cycle GO:0000278 IMP
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    protein localization to kinetochore GO:0034501 IMP
Subcellular Localization
    nuclear chromosome GO:0000228 IMP
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    nuclear speck GO:0016607 IDA
    U2-type precatalytic spliceosome GO:0071005 IDA
    mitotic spindle pole GO:0097431 IDA
 Experiment description of studies that identified IK in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 TTC14  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 CDK11B 984
Co-fractionation Homo sapiens
5 QARS 5859
Two-hybrid Homo sapiens
6 SNRPE 6635
Affinity Capture-MS Homo sapiens
7 COIL  
Proximity Label-MS Homo sapiens
8 PPIE 10450
Affinity Capture-MS Homo sapiens
9 TOP2B 7155
Co-fractionation Homo sapiens
10 NUP62 23636
Two-hybrid Homo sapiens
11 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
12 ACTC1 70
Proximity Label-MS Homo sapiens
13 CTNNBL1 56259
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
14 DHX8 1659
Proximity Label-MS Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 ATM 472
Affinity Capture-RNA Homo sapiens
17 TRIM55  
Two-hybrid Homo sapiens
18 SNRPF 6636
Affinity Capture-MS Homo sapiens
19 APP 351
Reconstituted Complex Homo sapiens
20 APEX1 328
Proximity Label-MS Homo sapiens
21 C9orf78 51759
Affinity Capture-MS Homo sapiens
22 Pqbp1  
Affinity Capture-MS Mus musculus
23 MRFAP1L1  
Affinity Capture-MS Homo sapiens
24 SRCAP  
Co-fractionation Homo sapiens
25 RNPS1 10921
Affinity Capture-MS Homo sapiens
26 CEP70  
Two-hybrid Homo sapiens
27 NOLC1 9221
Co-fractionation Homo sapiens
28 FRA10AC1 118924
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
29 SRSF10 10772
Co-fractionation Homo sapiens
30 WDR18 57418
Co-fractionation Homo sapiens
31 PRC1 9055
Affinity Capture-MS Homo sapiens
32 SNRPC 6631
Affinity Capture-MS Homo sapiens
33 OBSL1 23363
Affinity Capture-MS Homo sapiens
34 EZH1  
Affinity Capture-MS Homo sapiens
35 SRSF3 6428
Co-fractionation Homo sapiens
36 ARID1A 8289
Negative Genetic Homo sapiens
37 U2AF1 7307
Co-fractionation Homo sapiens
38 ATG16L1 55054
Affinity Capture-MS Homo sapiens
39 DDX6 1656
Two-hybrid Homo sapiens
40 RAB8B 51762
Affinity Capture-MS Homo sapiens
41 PFN2 5217
Two-hybrid Homo sapiens
42 MECP2 4204
Affinity Capture-MS Homo sapiens
43 MYCN  
Affinity Capture-MS Homo sapiens
44 CHD3 1107
Two-hybrid Homo sapiens
45 THOC1 9984
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
46 NCSTN 23385
Co-fractionation Homo sapiens
47 RBM14 10432
Co-fractionation Homo sapiens
48 KIF14 9928
Affinity Capture-MS Homo sapiens
49 VTN 7448
Co-fractionation Homo sapiens
50 UFL1 23376
Affinity Capture-MS Homo sapiens
51 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
52 DDX21 9188
Co-fractionation Homo sapiens
53 FKBP6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SNRPA 6626
Affinity Capture-MS Homo sapiens
55 SMU1 55234
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
56 IFI27L1  
Affinity Capture-MS Homo sapiens
57 SUPT16H 11198
Co-fractionation Homo sapiens
58 IK 3550
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
59 SAFB2 9667
Co-fractionation Homo sapiens
60 OGT 8473
Reconstituted Complex Homo sapiens
61 RPL31 6160
Proximity Label-MS Homo sapiens
62 RPS7 6201
Co-fractionation Homo sapiens
63 U2AF2 11338
Co-fractionation Homo sapiens
64 RAD18  
Affinity Capture-MS Homo sapiens
65 LUC7L2 51631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ARRDC3 57561
Affinity Capture-MS Homo sapiens
67 RPA4  
Proximity Label-MS Homo sapiens
68 HECTD1 25831
Affinity Capture-MS Homo sapiens
69 MYBBP1A 10514
Co-fractionation Homo sapiens
70 DUSP23 54935
Affinity Capture-MS Homo sapiens
71 CEBPA  
Protein-peptide Homo sapiens
72 TRIM63  
Two-hybrid Homo sapiens
73 Asna1 56495
Affinity Capture-MS Mus musculus
74 RBM10  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
75 CD3EAP  
Proximity Label-MS Homo sapiens
76 ZNF326 284695
Co-fractionation Homo sapiens
77 CALML3 810
Affinity Capture-MS Homo sapiens
78 SRSF11 9295
Co-fractionation Homo sapiens
79 NTRK1 4914
Affinity Capture-MS Homo sapiens
80 PRPF4B 8899
Affinity Capture-MS Homo sapiens
81 C11orf57  
Affinity Capture-MS Homo sapiens
82 MSH2 4436
Co-fractionation Homo sapiens
83 XPO1 7514
Affinity Capture-MS Homo sapiens
84 EGLN3  
Two-hybrid Homo sapiens
85 TPR 7175
Co-fractionation Homo sapiens
86 SMARCA5 8467
Co-fractionation Homo sapiens
87 SIRT7  
Affinity Capture-MS Homo sapiens
88 SNRPB 6628
Affinity Capture-MS Homo sapiens
89 SRSF1 6426
Co-fractionation Homo sapiens
90 CERK 64781
Affinity Capture-MS Homo sapiens
91 LOXL2 4017
Affinity Capture-MS Homo sapiens
92 XAB2 56949
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
93 SRSF5 6430
Co-fractionation Homo sapiens
94 EGFR 1956
Negative Genetic Homo sapiens
95 TSSC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 EFTUD2 9343
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
97 RPA3 6119
Proximity Label-MS Homo sapiens
98 MFAP1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
99 MYC  
Affinity Capture-MS Homo sapiens
100 USP47 55031
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
101 MRFAP1  
Affinity Capture-MS Homo sapiens
102 TOP1 7150
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
103 KAT5  
Two-hybrid Homo sapiens
104 TOLLIP 54472
Affinity Capture-MS Homo sapiens
105 KDM1A 23028
Two-hybrid Homo sapiens
106 CUL7 9820
Affinity Capture-MS Homo sapiens
107 DDRGK1 65992
Affinity Capture-MS Homo sapiens
108 RNF2  
Affinity Capture-MS Homo sapiens
109 BMI1  
Affinity Capture-MS Homo sapiens
110 SEPT7 989
Co-fractionation Homo sapiens
111 C9orf72  
Affinity Capture-MS Homo sapiens
112 PPP2CB 5516
Affinity Capture-MS Homo sapiens
113 PHGDH 26227
Co-fractionation Homo sapiens
114 PPP2R5E 5529
Co-fractionation Homo sapiens
115 TOE1 114034
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
116 GRB2 2885
Two-hybrid Homo sapiens
117 FBXL6  
Proximity Label-MS Homo sapiens
118 FANCC 2176
Two-hybrid Homo sapiens
119 WWOX 51741
Affinity Capture-MS Homo sapiens
120 SAP18 10284
Co-fractionation Homo sapiens
121 PDHA1 5160
Affinity Capture-MS Homo sapiens
122 THRAP3 9967
Co-fractionation Homo sapiens
123 PRMT1 3276
Affinity Capture-MS Homo sapiens
124 PUF60 22827
Affinity Capture-MS Homo sapiens
125 DDX23 9416
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 MKI67  
Affinity Capture-MS Homo sapiens
127 NIFK 84365
Proximity Label-MS Homo sapiens
128 WIF1 11197
Affinity Capture-MS Homo sapiens
129 TRA2A 29896
Co-fractionation Homo sapiens
130 DHX40  
Proximity Label-MS Homo sapiens
131 SNRNP40 9410
Affinity Capture-MS Homo sapiens
132 SNIP1  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
133 MND1  
Affinity Capture-MS Homo sapiens
134 SRSF7 6432
Co-fractionation Homo sapiens
135 ZCCHC10  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
136 RANBP2 5903
Co-fractionation Homo sapiens
137 PRPF6 24148
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
138 SNW1 22938
Affinity Capture-MS Homo sapiens
139 HNRNPU 3192
Co-fractionation Homo sapiens
140 GNB2L1 10399
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
141 AR 367
Proximity Label-MS Homo sapiens
142 RNF113A 7737
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
143 Supt5  
Two-hybrid Mus musculus
144 ZNF830  
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Two-hybrid Homo sapiens
145 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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