Gene description for Asna1
Gene name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Gene symbol Asna1
Other names/aliases 1810048H22Rik
ArsA
Species Mus musculus
 Database cross references - Asna1
ExoCarta ExoCarta_56495
Vesiclepedia VP_56495
Entrez Gene 56495
UniProt O54984  
 Asna1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Asna1
Molecular Function
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IEA
    membrane insertase activity GO:0032977 IEA
    membrane insertase activity GO:0032977 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 ISO
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IBA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 ISO
    tail-anchored membrane protein insertion into ER membrane GO:0071816 ISS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    GET complex GO:0043529 IBA
    GET complex GO:0043529 ISO
    GET complex GO:0043529 ISS
 Experiment description of studies that identified Asna1 in exosomes
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
EM
Biophysical techniques
GAPDH|UCHL1|HSP90
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Asna1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fitm2  
Positive Genetic Mus musculus
2 Vamp2  
Co-fractionation Mus musculus
3 BAG6 7917
Affinity Capture-MS Homo sapiens
4 IVNS1ABP  
Affinity Capture-MS Homo sapiens
5 Tubb5 22154
Co-fractionation Mus musculus
6 UBL4A 8266
Affinity Capture-MS Homo sapiens
7 Eed  
Affinity Capture-MS Mus musculus
8 Ruvbl2 20174
Co-fractionation Mus musculus
9 IK 3550
Affinity Capture-MS Homo sapiens
10 Tfe3  
Affinity Capture-MS Mus musculus
11 BMPR2 659
Affinity Capture-MS Homo sapiens
12 Vapb  
Affinity Capture-Western Mus musculus
13 GET4 51608
Affinity Capture-MS Homo sapiens
14 Sgta 52551
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Asna1 is involved
No pathways found





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