Gene description for GET4
Gene name golgi to ER traffic protein 4 homolog (S. cerevisiae)
Gene symbol GET4
Other names/aliases C7orf20
CEE
CGI-20
TRC35
Species Homo sapiens
 Database cross references - GET4
ExoCarta ExoCarta_51608
Vesiclepedia VP_51608
Entrez Gene 51608
HGNC 21690
MIM 612056
UniProt Q7L5D6  
 GET4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for GET4
Molecular Function
    protein binding GO:0005515 IPI
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 IDA
    regulation of protein stability GO:0031647 IDA
    ERAD pathway GO:0036503 IMP
    maintenance of unfolded protein GO:0036506 IMP
    protein insertion into ER membrane GO:0045048 IBA
    protein insertion into ER membrane GO:0045048 IMP
    cytoplasmic sequestering of protein GO:0051220 IMP
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IDA
    tail-anchored membrane protein insertion into ER membrane GO:0071816 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    chromosome GO:0005694 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 NAS
    BAT3 complex GO:0071818 IBA
    BAT3 complex GO:0071818 IDA
    BAT3 complex GO:0071818 IPI
 Experiment description of studies that identified GET4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GET4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENKD1  
Two-hybrid Homo sapiens
2 ICOSLG  
Affinity Capture-MS Homo sapiens
3 UBL4A 8266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
4 EEF1G 1937
Two-hybrid Homo sapiens
5 USP13  
Affinity Capture-Western Homo sapiens
6 RABEP2 79874
Co-fractionation Homo sapiens
7 MYEOV  
Affinity Capture-MS Homo sapiens
8 TARDBP 23435
Affinity Capture-Western Homo sapiens
9 GIT2 9815
Two-hybrid Homo sapiens
10 TTR 7276
Two-hybrid Homo sapiens
11 DNAJB2 3300
Affinity Capture-MS Homo sapiens
12 PAICS 10606
Co-fractionation Homo sapiens
13 TUBB8 347688
Affinity Capture-MS Homo sapiens
14 C9orf78 51759
Affinity Capture-MS Homo sapiens
15 HSP90AB1 3326
Co-fractionation Homo sapiens
16 SLC25A48  
Affinity Capture-MS Homo sapiens
17 ASXL1  
Affinity Capture-MS Homo sapiens
18 ZP3 7784
Affinity Capture-MS Homo sapiens
19 CDKN1A  
Two-hybrid Homo sapiens
20 RDX 5962
Co-fractionation Homo sapiens
21 NPLOC4 55666
Affinity Capture-MS Homo sapiens
22 IL1RL2 8808
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HERC2 8924
Affinity Capture-MS Homo sapiens
24 FAF1 11124
Affinity Capture-MS Homo sapiens
25 RNF126  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 SDCBP 6386
Two-hybrid Homo sapiens
27 RAB3A 5864
Affinity Capture-MS Homo sapiens
28 PLS3 5358
Co-fractionation Homo sapiens
29 CCT5 22948
Co-fractionation Homo sapiens
30 IQCF2  
Affinity Capture-MS Homo sapiens
31 EXOC2 55770
Co-fractionation Homo sapiens
32 MTCH2 23788
Proximity Label-MS Homo sapiens
33 IRAK1  
Affinity Capture-MS Homo sapiens
34 FBXW12  
Affinity Capture-MS Homo sapiens
35 SCN1B  
Affinity Capture-MS Homo sapiens
36 AMFR 267
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
37 CSTF2 1478
Co-fractionation Homo sapiens
38 TUBA1A 7846
Affinity Capture-MS Homo sapiens
39 WRAP73 49856
Affinity Capture-MS Homo sapiens
40 BAG6 7917
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
41 ASNA1 439
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
42 TIGIT  
Affinity Capture-MS Homo sapiens
43 TUBA1C 84790
Affinity Capture-MS Homo sapiens
44 BTF3 689
Affinity Capture-MS Homo sapiens
45 VCP 7415
Affinity Capture-MS Homo sapiens
46 GDPD5 81544
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 KCNA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PLK1 5347
Two-hybrid Homo sapiens
50 NOMO3 408050
Affinity Capture-MS Homo sapiens
51 UBC 7316
Affinity Capture-MS Homo sapiens
52 PRMT2  
Affinity Capture-MS Homo sapiens
53 IL17RC 84818
Affinity Capture-MS Homo sapiens
54 CEBPA  
Protein-peptide Homo sapiens
55 SEC61B 10952
Reconstituted Complex Homo sapiens
56 Asna1 56495
Affinity Capture-MS Mus musculus
57 DDX5 1655
Co-fractionation Homo sapiens
58 UFD1L 7353
Affinity Capture-MS Homo sapiens
59 LSM8 51691
Co-fractionation Homo sapiens
60 AHCY 191
Co-fractionation Homo sapiens
61 VAPB 9217
Affinity Capture-MS Homo sapiens
62 AIFM1 9131
Co-fractionation Homo sapiens
63 TAX1BP1 8887
Affinity Capture-MS Homo sapiens
64 ARRDC4  
Affinity Capture-MS Homo sapiens
65 CCT6A 908
Co-fractionation Homo sapiens
66 C1orf109  
Two-hybrid Homo sapiens
67 HERC2P9  
Affinity Capture-MS Homo sapiens
68 PRKAR1A 5573
Co-fractionation Homo sapiens
69 KRTAP9-3  
Two-hybrid Homo sapiens
70 TRA  
Affinity Capture-Western Homo sapiens
71 SNCAIP  
Two-hybrid Homo sapiens
72 CPNE9  
Affinity Capture-MS Homo sapiens
73 EGFR 1956
Negative Genetic Homo sapiens
74 C16orf72 29035
Affinity Capture-MS Homo sapiens
75 KIAA1377  
Two-hybrid Homo sapiens
76 SNX27 81609
Affinity Capture-MS Homo sapiens
77 WDR90  
Affinity Capture-MS Homo sapiens
78 SGTB  
Affinity Capture-MS Homo sapiens
79 PRKCSH 5589
Two-hybrid Homo sapiens
80 MAPK6  
Affinity Capture-MS Homo sapiens
81 RPA3 6119
Proximity Label-MS Homo sapiens
82 KAT5  
Two-hybrid Homo sapiens
83 PSMC5 5705
Affinity Capture-MS Homo sapiens
84 DUSP21  
Two-hybrid Homo sapiens
85 SGTA 6449
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Co-crystal Structure Homo sapiens
86 PRKAB2 5565
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 GABRD  
Affinity Capture-MS Homo sapiens
88 UBXN1 51035
Affinity Capture-MS Homo sapiens
89 HUWE1 10075
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 MTA2 9219
Co-fractionation Homo sapiens
91 FBN1 2200
Two-hybrid Homo sapiens
92 KIF2A 3796
Affinity Capture-MS Homo sapiens
93 FASN 2194
Negative Genetic Homo sapiens
94 NSUN5P1  
Affinity Capture-MS Homo sapiens
95 FBXL6  
Affinity Capture-MS Homo sapiens
96 CDK8 1024
Affinity Capture-MS Homo sapiens
97 FUZ 80199
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 ABCA9 10350
Affinity Capture-MS Homo sapiens
99 FLYWCH2  
Two-hybrid Homo sapiens
100 UBL4B  
Affinity Capture-MS Homo sapiens
101 KEAP1 9817
Affinity Capture-MS Homo sapiens
102 EDEM1  
Affinity Capture-MS Homo sapiens
103 TCAF1 9747
Affinity Capture-MS Homo sapiens
104 ERLIN2 11160
Affinity Capture-MS Homo sapiens
105 CAPNS1 826
Two-hybrid Homo sapiens
106 CATSPERD  
Affinity Capture-MS Homo sapiens
107 MOV10 4343
Affinity Capture-RNA Homo sapiens
108 INF2 64423
Affinity Capture-MS Homo sapiens
109 EIF4B 1975
Co-fractionation Homo sapiens
110 VWA2 340706
Affinity Capture-MS Homo sapiens
111 HTR3E  
Affinity Capture-MS Homo sapiens
112 TCTEX1D4  
Affinity Capture-MS Homo sapiens
113 SHARPIN  
Affinity Capture-MS Homo sapiens
114 SERBP1 26135
Affinity Capture-MS Homo sapiens
115 TXNIP 10628
Affinity Capture-MS Homo sapiens
116 C9orf72  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
117 ATXN3 4287
Affinity Capture-MS Homo sapiens
118 NUP85 79902
Two-hybrid Homo sapiens
119 WARS 7453
Co-fractionation Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here