Gene description for RAB3A
Gene name RAB3A, member RAS oncogene family
Gene symbol RAB3A
Other names/aliases -
Species Homo sapiens
 Database cross references - RAB3A
ExoCarta ExoCarta_5864
Vesiclepedia VP_5864
Entrez Gene 5864
HGNC 9777
MIM 179490
UniProt P20336  
 RAB3A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for RAB3A
Molecular Function
    protein binding GO:0005515 IPI
    myosin V binding GO:0031489 IPI
    ATPase binding GO:0051117 IEA
    protein C-terminus binding GO:0008022 IEA
    GTPase binding GO:0051020 IEA
    GTPase activity GO:0003924 IMP
    GTP binding GO:0005525 IEA
    ATPase activator activity GO:0001671 IEA
Biological Process
    regulation of short-term neuronal synaptic plasticity GO:0048172 ISS
    protein localization to plasma membrane GO:0072659 IBA
    vesicle docking involved in exocytosis GO:0006904 IBA
    response to electrical stimulus GO:0051602 IEA
    intracellular protein transport GO:0006886 IBA
    regulation of synaptic vesicle fusion to presynaptic membrane GO:0031630 ISS
    protein secretion GO:0009306 IBA
    maintenance of presynaptic active zone structure GO:0048790 IEA
    sensory perception of touch GO:0050975 IEA
    mitochondrion organization GO:0007005 IEA
    respiratory system process GO:0003016 IEA
    positive regulation of ATPase activity GO:0032781 IEA
    lung development GO:0030324 IEA
    axonogenesis GO:0007409 IEA
    synaptic vesicle recycling GO:0036465 ISS
    glutamate secretion GO:0014047 TAS
    constitutive secretory pathway GO:0045054 TAS
    positive regulation of exocytosis GO:0045921 TAS
    synaptic transmission GO:0007268 TAS
    metabolic process GO:0008152 TAS
    Rab protein signal transduction GO:0032482 IBA
    neurotransmitter secretion GO:0007269 TAS
    positive regulation of regulated secretory pathway GO:1903307 IMP
    neuromuscular synaptic transmission GO:0007274 IEA
    synaptic vesicle maturation GO:0016188 IEA
    post-embryonic development GO:0009791 IEA
    synaptic vesicle exocytosis GO:0016079 ISS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    endosome GO:0005768 IBA
    synaptic vesicle GO:0008021 ISS
    clathrin-sculpted acetylcholine transport vesicle membrane GO:0060201 TAS
    protein complex GO:0043234 IEA
    acrosomal vesicle GO:0001669 IEA
    extracellular vesicle GO:1903561 IDA
    clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane GO:0061202 TAS
    secretory granule membrane GO:0030667 IBA
    terminal bouton GO:0043195 ISS
    axon GO:0030424 ISS
    clathrin-sculpted glutamate transport vesicle membrane GO:0060203 TAS
    cytosol GO:0005829 IEA
    clathrin-sculpted monoamine transport vesicle membrane GO:0070083 TAS
 Experiment description of studies that identified RAB3A in exosomes
1
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 274
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
EV Enriched markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
6
Experiment ID 193
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAB3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB3IP  
Affinity Capture-MS Homo sapiens
2 DMXL2  
Invivo Homo sapiens
3 BSN  
Affinity Capture-Western Homo sapiens
4 SYTL4 94121
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHML  
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 RAB3IL1 5866
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 RAB3A 5864
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SYTL5 94122
Invivo Homo sapiens
Invitro Homo sapiens
9 RABIF  
Reconstituted Complex Homo sapiens
10 RABAC1 10567
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 CHM 1121
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 RIMS2  
Affinity Capture-Western Homo sapiens
13 RPH3AL 9501
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
14 RIMS1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 RABGGTB 5876
Two-hybrid Homo sapiens
16 RPH3A 22895
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
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