Gene description for RAB3A
Gene name RAB3A, member RAS oncogene family
Gene symbol RAB3A
Other names/aliases -
Species Homo sapiens
 Database cross references - RAB3A
ExoCarta ExoCarta_5864
Vesiclepedia VP_5864
Entrez Gene 5864
HGNC 9777
MIM 179490
UniProt P20336  
 RAB3A identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for RAB3A
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    GTPase activity GO:0003924 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    protein-macromolecule adaptor activity GO:0030674 IEA
    GTP-dependent protein binding GO:0030742 IEA
    myosin V binding GO:0031489 IBA
    myosin V binding GO:0031489 IPI
Biological Process
    plasma membrane repair GO:0001778 IDA
    respiratory system process GO:0003016 IEA
    exocytosis GO:0006887 IDA
    mitochondrion organization GO:0007005 IEA
    neuromuscular synaptic transmission GO:0007274 IEA
    axonogenesis GO:0007409 IBA
    post-embryonic development GO:0009791 IEA
    synaptic vesicle exocytosis GO:0016079 IBA
    synaptic vesicle exocytosis GO:0016079 ISS
    synaptic vesicle maturation GO:0016188 IEA
    insulin secretion GO:0030073 IEA
    lung development GO:0030324 IEA
    regulation of synaptic vesicle fusion to presynaptic active zone membrane GO:0031630 ISS
    lysosome localization GO:0032418 IMP
    synaptic vesicle recycling GO:0036465 ISS
    constitutive secretory pathway GO:0045054 TAS
    regulated exocytosis GO:0045055 IMP
    positive regulation of exocytosis GO:0045921 TAS
    regulation of short-term neuronal synaptic plasticity GO:0048172 ISS
    synaptic vesicle transport GO:0048489 IEA
    maintenance of presynaptic active zone structure GO:0048790 IEA
    sensory perception of touch GO:0050975 IEA
    response to electrical stimulus GO:0051602 IEA
    acrosomal vesicle exocytosis GO:0060478 IBA
    acrosomal vesicle exocytosis GO:0060478 IDA
    evoked neurotransmitter secretion GO:0061670 IEA
    synaptic vesicle clustering GO:0097091 IEA
    regulation of presynaptic dense core granule exocytosis GO:0099161 IEA
    positive regulation of regulated secretory pathway GO:1903307 IMP
    regulation of plasma membrane repair GO:1905684 IMP
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    lysosome GO:0005764 IEA
    endosome GO:0005768 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IBA
    synaptic vesicle GO:0008021 ISS
    axon GO:0030424 IBA
    axon GO:0030424 ISS
    secretory granule membrane GO:0030667 TAS
    synaptic vesicle membrane GO:0030672 IBA
    terminal bouton GO:0043195 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    presynaptic active zone GO:0048786 ISS
    clathrin-sculpted acetylcholine transport vesicle membrane GO:0060201 TAS
    clathrin-sculpted glutamate transport vesicle membrane GO:0060203 TAS
    clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane GO:0061202 TAS
    clathrin-sculpted monoamine transport vesicle membrane GO:0070083 TAS
    presynapse GO:0098793 ISS
    postsynapse GO:0098794 ISS
    extracellular vesicle GO:1903561 HDA
 Experiment description of studies that identified RAB3A in exosomes
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
6
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAB3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Two-hybrid Homo sapiens
2 GPM6A 2823
Affinity Capture-MS Homo sapiens
3 SYTL4 94121
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHML  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SLC15A1  
Affinity Capture-MS Homo sapiens
7 RAB3D 9545
Affinity Capture-MS Homo sapiens
8 RTN4 57142
Affinity Capture-MS Homo sapiens
9 PSEN1 5663
Two-hybrid Homo sapiens
10 IBA57  
Affinity Capture-MS Homo sapiens
11 RPN2 6185
Affinity Capture-MS Homo sapiens
12 BSN  
Affinity Capture-Western Homo sapiens
13 RIMS1  
Reconstituted Complex Homo sapiens
14 MOCOS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 VWA5B2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ADRB2  
PCA Homo sapiens
18 DDOST 1650
Affinity Capture-MS Homo sapiens
19 SNCA 6622
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 RAB3IL1 5866
Two-hybrid Homo sapiens
21 CHRM4  
Two-hybrid Homo sapiens
22 SYTL5 94122
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 RANGRF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 RNF32  
Two-hybrid Homo sapiens
25 EGFR 1956
PCA Homo sapiens
26 RAB14 51552
Cross-Linking-MS (XL-MS) Homo sapiens
27 RAB3C 115827
Affinity Capture-MS Homo sapiens
28 VRTN  
Two-hybrid Homo sapiens
29 RABGGTB 5876
Two-hybrid Homo sapiens
30 FFAR1  
Affinity Capture-MS Homo sapiens
31 GET4 51608
Affinity Capture-MS Homo sapiens
32 A2M 2
Two-hybrid Homo sapiens
33 GDI2 2665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 EPHA8  
Affinity Capture-MS Homo sapiens
35 RAB3B 5865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SLC18A2  
Affinity Capture-MS Homo sapiens
37 Ccdc64b  
Affinity Capture-Western Mus musculus
38 RAB33B 83452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 TMED10 10972
Affinity Capture-MS Homo sapiens
40 RABAC1 10567
Two-hybrid Homo sapiens
41 KRTAP10-8  
Two-hybrid Homo sapiens
42 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RAB3IP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 AP1M2 10053
Two-hybrid Homo sapiens
46 GRSF1 2926
Two-hybrid Homo sapiens
47 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 WDR45B 56270
Affinity Capture-MS Homo sapiens
49 GDI1 2664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 RPN1 6184
Affinity Capture-MS Homo sapiens
51 HAX1  
Affinity Capture-MS Homo sapiens
52 RABGGTA 5875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RABIF  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 CHM 1121
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 MAST1  
Two-hybrid Homo sapiens
56 MLNR  
Affinity Capture-MS Homo sapiens
57 REP15  
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
58 C3orf52  
Affinity Capture-MS Homo sapiens
59 ECSIT 51295
Two-hybrid Homo sapiens
60 OST4  
Affinity Capture-MS Homo sapiens
61 RPH3A 22895
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
View the network image/svg+xml



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