Gene description for AP1M2
Gene name adaptor-related protein complex 1, mu 2 subunit
Gene symbol AP1M2
Other names/aliases AP1-mu2
HSMU1B
MU-1B
MU1B
mu2
Species Homo sapiens
 Database cross references - AP1M2
ExoCarta ExoCarta_10053
Vesiclepedia VP_10053
Entrez Gene 10053
HGNC 558
MIM 607309
UniProt Q9Y6Q5  
 AP1M2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for AP1M2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    regulation of defense response to virus by virus GO:0050690 TAS
    membrane organization GO:0061024 TAS
    vesicle targeting GO:0006903 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    viral process GO:0016032 TAS
    post-Golgi vesicle-mediated transport GO:0006892 TAS
    protein targeting GO:0006605 TAS
Subcellular Localization
    cytoplasmic vesicle membrane GO:0030659 TAS
    cytosol GO:0005829 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    clathrin-coated endocytic vesicle membrane GO:0030669 TAS
    clathrin adaptor complex GO:0030131 IEA
    lysosomal membrane GO:0005765 TAS
    Golgi membrane GO:0000139 TAS
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified AP1M2 in exosomes
1
Experiment ID 282
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
EV Enriched markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 211
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
MISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
EV Enriched markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 191
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD81|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AP1M2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP1B1 162
Invivo Homo sapiens
2 TGOLN2 10618
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 LDLR 3949
Affinity Capture-Western Homo sapiens
4 AP1G1 164
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
5 PVR 5817
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
6 GAK 2580
Affinity Capture-MS Homo sapiens
7 AP2B1 163
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
8 AP1M1 8907
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
9 AP1S1 1174
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
10 EHD2 30846
Reconstituted Complex Homo sapiens
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