ExoCarta: Gene summary

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Gene description for AP1M2
Gene name	adaptor-related protein complex 1, mu 2 subunit
Gene symbol	AP1M2
Other names/aliases	AP1-mu2 HSMU1B MU-1B MU1B mu2
Species	Homo sapiens
Database cross references - AP1M2
ExoCarta	ExoCarta_10053
Vesiclepedia	VP_10053
Entrez Gene	10053
HGNC	558
MIM	607309
UniProt	Q9Y6Q5
AP1M2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells	25890246
Colorectal cancer cells	25890246
Colorectal cancer cells	34887515
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Squamous carcinoma cells	20124223

Gene ontology annotations for AP1M2

Molecular Function
	protein binding	GO:0005515	IPI
	clathrin adaptor activity	GO:0035615	IBA
Biological Process
	protein targeting	GO:0006605	TAS
	intracellular protein transport	GO:0006886	IEA
	vesicle targeting	GO:0006903	TAS
	vesicle-mediated transport	GO:0016192	IBA
	vesicle-mediated transport	GO:0016192	NAS
	basolateral protein secretion	GO:0110010	NAS
Subcellular Localization
	Golgi membrane	GO:0000139	TAS
	lysosomal membrane	GO:0005765	TAS
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	AP-1 adaptor complex	GO:0030121	NAS
	clathrin-coated vesicle	GO:0030136	IBA
	cytoplasmic vesicle membrane	GO:0030659	TAS
	trans-Golgi network membrane	GO:0032588	NAS
	trans-Golgi network membrane	GO:0032588	TAS
	intracellular membrane-bounded organelle	GO:0043231	IDA

Experiment description of studies that identified AP1M2 in exosomes

Experiment ID

282

MISEV standards
✔ CEM	Biophysical techniques
✔ Alix\|TSG101\|CD63\|CD81\|EpCAM	Enriched markers
✘	Negative markers
✔ DLS	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25890246

Organism

Homo sapiens

Experiment description

Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.

Authors

"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."

Journal name

Methods

Publication year

2015

Sample

Colorectal cancer cells

Sample name

LIM1863 - Ultracentrifugation - Rep 1

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry
Western blotting

Experiment ID

283

MISEV standards
✔ CEM	Biophysical techniques
✔ Alix\|TSG101\|CD63\|CD81\|EpCAM	Enriched markers
✘	Negative markers
✔ DLS	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

25890246

Organism

Homo sapiens

Experiment description

Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.

Authors

"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."

Journal name

Methods

Publication year

2015

Sample

Colorectal cancer cells

Sample name

LIM1863 - Ultracentrifugation - Rep 2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry
Western blotting

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

191

MISEV standards
✘	Biophysical techniques
✔ Alix\|CD81\|CD9	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

20124223

Organism

Homo sapiens

Experiment description

Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.

Authors

"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."

Journal name

Mol Cell Proteomics

Publication year

2010

Sample

Squamous carcinoma cells

Sample name

Squamous carcinoma cell (A431)

Isolation/purification methods

Differential centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for AP1M2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

SYNRG

11276

Affinity Capture-MS

Homo sapiens

A2M

Two-hybrid

Homo sapiens

TMF1

7110

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SCNN1G

Affinity Capture-Western

Homo sapiens

TUBA1A

7846

Affinity Capture-MS

Homo sapiens

AP2B1

163

Two-hybrid		Homo sapiens
Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RAB3D

9545

Two-hybrid

Homo sapiens

AP1M1

8907

Affinity Capture-MS

Homo sapiens

PSEN1

5663

Two-hybrid

Homo sapiens

SCNN1A

Affinity Capture-Western

Homo sapiens

MSH2

4436

Co-fractionation

Homo sapiens

OPALIN

Affinity Capture-MS

Homo sapiens

AP1S2

8905

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

TFAP2A

Affinity Capture-Western

Homo sapiens

LRRC25

Affinity Capture-MS

Homo sapiens

KIF13A

63971

Affinity Capture-MS

Homo sapiens

NECAP1

25977

Affinity Capture-MS

Homo sapiens

ATRN

8455

Affinity Capture-MS

Homo sapiens

PRKACG

Affinity Capture-MS

Homo sapiens

MON1A

84315

Co-fractionation

Homo sapiens

EGFR

1956

Affinity Capture-MS

Homo sapiens

LOXL4

84171

Two-hybrid

Homo sapiens

ERBB3

2065

Affinity Capture-MS

Homo sapiens

RIPK1

8737

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

EHD2

30846

Reconstituted Complex

Homo sapiens

AFTPH

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

APP

351

Two-hybrid

Homo sapiens

SPACA1

81833

Affinity Capture-MS

Homo sapiens

AP1G2

8906

Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

AP1S3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MGRN1

23295

Affinity Capture-MS

Homo sapiens

HEATR5B

54497

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDRG1

10397

Affinity Capture-MS

Homo sapiens

EPN1

29924

Affinity Capture-Western

Homo sapiens

BLMH

642

Two-hybrid

Homo sapiens

PTGES3

10728

Affinity Capture-MS

Homo sapiens

AP1B1

162

Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LDLR

3949

Affinity Capture-Western

Homo sapiens

MEA1

Affinity Capture-MS

Homo sapiens

AP1G1

164

Affinity Capture-Western		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PVR

5817

Two-hybrid

Homo sapiens

367

Affinity Capture-MS

Homo sapiens

HECTD1

25831

Affinity Capture-MS

Homo sapiens

RAB3A

5864

Two-hybrid

Homo sapiens

SCNN1B

6338

Affinity Capture-Western

Homo sapiens

ANKRD46

157567

Affinity Capture-MS

Homo sapiens

MAST1

Two-hybrid

Homo sapiens

AP1S1

1174

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ECSIT

51295

Two-hybrid

Homo sapiens

HLA-C

3107

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which AP1M2 is involved

Pathway	Evidence	Source
Adaptive Immune System	TAS	Reactome
Disease	TAS	Reactome
Golgi Associated Vesicle Biogenesis	TAS	Reactome
HIV Infection	TAS	Reactome
Host Interactions of HIV factors	TAS	Reactome
Immune System	TAS	Reactome
Infectious disease	TAS	Reactome
Lysosome Vesicle Biogenesis	TAS	Reactome
Membrane Trafficking	TAS	Reactome
MHC class II antigen presentation	TAS	Reactome
Nef mediated downregulation of MHC class I complex cell surface expression	TAS	Reactome
Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters	TAS	Reactome
The role of Nef in HIV-1 replication and disease pathogenesis	TAS	Reactome
trans-Golgi Network Vesicle Budding	TAS	Reactome
Vesicle-mediated transport	TAS	Reactome
Viral Infection Pathways	TAS	Reactome

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