Gene description for EPN1
Gene name epsin 1
Gene symbol EPN1
Other names/aliases -
Species Homo sapiens
 Database cross references - EPN1
ExoCarta ExoCarta_29924
Vesiclepedia VP_29924
Entrez Gene 29924
HGNC 21604
MIM 607262
UniProt Q9Y6I3  
 EPN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for EPN1
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    clathrin binding GO:0030276 IBA
    molecular sequestering activity GO:0140313 EXP
    molecular sequestering activity GO:0140313 IPI
Biological Process
    in utero embryonic development GO:0001701 IEA
    endocytosis GO:0006897 IBA
    Notch signaling pathway GO:0007219 IEA
    female pregnancy GO:0007565 IEA
    embryonic organ development GO:0048568 IEA
    negative regulation of sprouting angiogenesis GO:1903671 IGI
Subcellular Localization
    nucleus GO:0005634 IEA
    endosome GO:0005768 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IEA
    clathrin vesicle coat GO:0030125 IBA
 Experiment description of studies that identified EPN1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EPN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP2B1 163
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
2 OCLN 100506658
Affinity Capture-Western Homo sapiens
3 SCNN1A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 EPN3 55040
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 S100A6 6277
Affinity Capture-MS Homo sapiens
7 FBXO25  
Biochemical Activity Homo sapiens
8 EPS15 2060
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
9 MAP4 4134
Affinity Capture-MS Homo sapiens
10 KIF20A 10112
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
12 FPR1  
Affinity Capture-MS Homo sapiens
13 CLTB 1212
Proximity Label-MS Homo sapiens
14 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
15 B3GAT1  
Proximity Label-MS Homo sapiens
16 LRP6 4040
Affinity Capture-Western Homo sapiens
17 PTPN12 5782
Co-fractionation Homo sapiens
18 H1FX 8971
Affinity Capture-MS Homo sapiens
19 AP2A1 160
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
20 KDR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 CXCR4 7852
Affinity Capture-MS Homo sapiens
22 EPS15L1 58513
Affinity Capture-Western Homo sapiens
23 ITGA4 3676
Affinity Capture-MS Homo sapiens
24 CRMP1 1400
Two-hybrid Homo sapiens
25 VCAM1 7412
Affinity Capture-MS Homo sapiens
26 GTSE1 51512
Affinity Capture-MS Homo sapiens
27 LDLRAD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 SMAD5 4090
Co-fractionation Homo sapiens
29 GTF3A 2971
Affinity Capture-Western Homo sapiens
30 FZD7 8324
Affinity Capture-Western Homo sapiens
31 DVL2 1856
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 LAMTOR1 55004
Proximity Label-MS Homo sapiens
33 TFAP2A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 H2AFZ 3015
Affinity Capture-MS Homo sapiens
35 BCAR1 9564
Co-fractionation Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 ARRDC1 92714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 FZD8 8325
Affinity Capture-Western Homo sapiens
39 LAPTM5 7805
Two-hybrid Homo sapiens
40 ERBB3 2065
Affinity Capture-Western Homo sapiens
41 EHD2 30846
Affinity Capture-Western Homo sapiens
42 DAZAP2  
Two-hybrid Homo sapiens
43 PLEKHB2 55041
Two-hybrid Homo sapiens
44 CCNB1 891
Co-fractionation Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 AP1B1 162
Affinity Capture-Western Homo sapiens
47 TMEM45B 120224
Affinity Capture-MS Homo sapiens
48 REPS2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Far Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 PIK3R1 5295
Affinity Capture-Western Homo sapiens
50 TECR 9524
Affinity Capture-MS Homo sapiens
51 MED31  
Two-hybrid Homo sapiens
52 EHD1 10938
Affinity Capture-Western Homo sapiens
53 SCNN1G  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
54 FAM189B  
Affinity Capture-MS Homo sapiens
55 SLC25A12 8604
Affinity Capture-MS Homo sapiens
56 XPO1 7514
Affinity Capture-MS Homo sapiens
57 FLVCR2 55640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 BAG2 9532
Affinity Capture-MS Homo sapiens
59 UBQLN2 29978
Affinity Capture-Western Homo sapiens
60 LAMP1 3916
Affinity Capture-MS Homo sapiens
61 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 MAP3K1 4214
Biochemical Activity Homo sapiens
63 KIAA1377  
Two-hybrid Homo sapiens
64 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 SART1 9092
Affinity Capture-MS Homo sapiens
66 FN1 2335
Affinity Capture-MS Homo sapiens
67 NXF1 10482
Affinity Capture-RNA Homo sapiens
68 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SLC2A14 144195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
71 AP2A2 161
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
72 HSPA1A 3303
Affinity Capture-MS Homo sapiens
73 AP1M2 10053
Affinity Capture-Western Homo sapiens
74 DLL1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
75 PARP1 142
Affinity Capture-MS Homo sapiens
76 RHOB 388
Proximity Label-MS Homo sapiens
77 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
78 LPPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 TADA2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 PHGDH 26227
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 RALBP1 10928
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 RPS27A 6233
Affinity Capture-MS Homo sapiens
83 RNF11 26994
Two-hybrid Homo sapiens
84 FLT4  
Affinity Capture-Western Homo sapiens
85 IRS2 8660
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
86 SMURF1 57154
Biochemical Activity Homo sapiens
87 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
88 SLC6A3  
Affinity Capture-Western Homo sapiens
89 SCNN1B 6338
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
90 CDK1 983
Biochemical Activity Homo sapiens
91 C10orf88  
Affinity Capture-MS Homo sapiens
92 MOV10 4343
Affinity Capture-RNA Homo sapiens
93 KDELR2 11014
Affinity Capture-MS Homo sapiens
94 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RAB33B 83452
Affinity Capture-MS Homo sapiens
96 KRAS 3845
Proximity Label-MS Homo sapiens
97 GAS2L1 10634
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NOTCH1 4851
Affinity Capture-Western Homo sapiens
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