Gene description for CXCR4
Gene name chemokine (C-X-C motif) receptor 4
Gene symbol CXCR4
Other names/aliases CD184
D2S201E
FB22
HM89
HSY3RR
LAP-3
LAP3
LCR1
LESTR
NPY3R
NPYR
NPYRL
NPYY3R
WHIM
WHIMS
Species Homo sapiens
 Database cross references - CXCR4
ExoCarta ExoCarta_7852
Entrez Gene 7852
HGNC 2561
MIM 162643
UniProt P61073  
 CXCR4 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
T cells 11907077    
 Gene ontology annotations for CXCR4
Molecular Function
    virus receptor activity GO:0001618 IEA
    cytokine binding GO:0019955 IEA
    actin binding GO:0003779 IDA
    ubiquitin protein ligase binding GO:0031625 IPI
    myosin light chain binding GO:0032027 IDA
    coreceptor activity GO:0015026 TAS
    ubiquitin binding GO:0043130 IDA
    C-X-C chemokine receptor activity GO:0016494 NAS
    protein binding GO:0005515 IPI
    G-protein coupled receptor activity GO:0004930 TAS
Biological Process
    germ cell development GO:0007281 IEA
    dendritic cell chemotaxis GO:0002407 TAS
    activation of MAPK activity GO:0000187 TAS
    neuron migration GO:0001764 IEA
    brain development GO:0007420 IEA
    patterning of blood vessels GO:0001569 IEA
    positive regulation of oligodendrocyte differentiation GO:0048714 ISS
    entry into host cell GO:0030260 TAS
    neutrophil activation GO:0042119 IEA
    ameboidal-type cell migration GO:0001667 IEA
    viral process GO:0016032 TAS
    chemokine-mediated signaling pathway GO:0070098 NAS
    motor neuron axon guidance GO:0008045 IEA
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    positive regulation of cytosolic calcium ion concentration GO:0007204 TAS
    calcium-mediated signaling GO:0019722 IMP
    viral entry into host cell GO:0046718 IEA
    response to virus GO:0009615 TAS
    germ cell migration GO:0008354 IEA
    neural precursor cell proliferation GO:0061351 IEA
    response to hypoxia GO:0001666 IEP
    apoptotic process GO:0006915 TAS
    inflammatory response GO:0006954 TAS
    regulation of chemotaxis GO:0050920 IMP
    regulation of cell migration GO:0030334 IEA
    T cell proliferation GO:0042098 IEA
    myelin maintenance GO:0043217 ISS
    cellular response to cytokine stimulus GO:0071345 IDA
Subcellular Localization
    cell surface GO:0009986 IDA
    growth cone GO:0030426 IEA
    cell-cell junction GO:0005911 IEA
    lysosome GO:0005764 IDA
    early endosome GO:0005769 IDA
    cytoplasmic membrane-bounded vesicle GO:0016023 IDA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 TAS
    cytoplasmic vesicle GO:0031410 IDA
    integral component of membrane GO:0016021 IEA
    cytoplasm GO:0005737 TAS
    cell leading edge GO:0031252 IDA
    plasma membrane GO:0005886 TAS
    late endosome GO:0005770 IDA
    external side of plasma membrane GO:0009897 IEA
 Experiment description of studies that identified CXCR4 in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 18
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method FACS
PubMed ID 11907077    
Organism Homo sapiens
Experiment description TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex.
Authors Blanchard N, Lankar D, Faure F, Regnault A, Dumont C, Raposo G, Hivroz C
Journal name JIMMU
Publication year 2002
Sample T cells
Sample name Jurkat
T cell blasts
Isolation/purification methods Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study FACS
 Protein-protein interactions for CXCR4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD4 920
Affinity Capture-Western Homo sapiens
2 JAK3 3718
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 SOCS3 9021
Affinity Capture-Western Homo sapiens
4 JAK2 3717
Affinity Capture-Western Homo sapiens
5 CCR5 1234
Reconstituted Complex Homo sapiens
6 PTK2 5747
Affinity Capture-Western Homo sapiens
7 ADRBK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 PTPN6 5777
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 CXCL12  
Invitro Homo sapiens
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