Gene description for ATP2B3
Gene name ATPase, Ca++ transporting, plasma membrane 3
Gene symbol ATP2B3
Other names/aliases CFAP39
CLA2
OPCA
PMCA3
PMCA3a
SCAX1
Species Homo sapiens
 Database cross references - ATP2B3
ExoCarta ExoCarta_492
Vesiclepedia VP_492
Entrez Gene 492
HGNC 816
MIM 300014
UniProt Q16720  
 ATP2B3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ATP2B3
Molecular Function
    P-type calcium transporter activity GO:0005388 IBA
    P-type calcium transporter activity GO:0005388 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    calcium ion transmembrane transporter activity GO:0015085 IDA
    ATP hydrolysis activity GO:0016887 IEA
    metal ion binding GO:0046872 IEA
    P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration GO:1905056 IDA
    P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration GO:1905056 IMP
    P-type calcium transporter activity involved in regulation of presynaptic cytosolic calcium ion concentration GO:1905056 NAS
Biological Process
    monoatomic ion transmembrane transport GO:0034220 TAS
    regulation of cytosolic calcium ion concentration GO:0051480 IBA
    regulation of cytosolic calcium ion concentration GO:0051480 IDA
    regulation of presynaptic cytosolic calcium ion concentration GO:0099509 IEA
    regulation of cardiac conduction GO:1903779 TAS
    calcium ion export across plasma membrane GO:1990034 IDA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    presynaptic membrane GO:0042734 ISS
    intracellular membrane-bounded organelle GO:0043231 IBA
    parallel fiber to Purkinje cell synapse GO:0098688 ISS
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 NAS
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
    GABA-ergic synapse GO:0098982 NAS
    extracellular vesicle GO:1903561 HDA
    parallel fiber GO:1990032 ISS
 Experiment description of studies that identified ATP2B3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ATP2B3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VCAM1 7412
Affinity Capture-MS Homo sapiens
2 SLC19A1 6573
Affinity Capture-MS Homo sapiens
3 GPM6A 2823
Affinity Capture-MS Homo sapiens
4 RNF43  
Proximity Label-MS Homo sapiens
5 P2RY2 5029
Affinity Capture-MS Homo sapiens
6 UBE2N 7334
Co-fractionation Homo sapiens
7 KIF23 9493
Affinity Capture-MS Homo sapiens
8 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ADAM7  
Affinity Capture-MS Homo sapiens
10 HSD17B6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 TERF2  
Affinity Capture-MS Homo sapiens
12 PNPLA4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LGR4 55366
Affinity Capture-MS Homo sapiens
14 CLGN 1047
Co-fractionation Homo sapiens
15 TMEM59 9528
Affinity Capture-MS Homo sapiens
16 CANX 821
Co-fractionation Homo sapiens
17 CXCR4 7852
Affinity Capture-MS Homo sapiens
18 CALML3 810
Affinity Capture-MS Homo sapiens
19 TXNDC15  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 LILRB4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ATP2B1 490
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
23 LRRC55  
Affinity Capture-MS Homo sapiens
24 GALNT6 11226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 NPTN 27020
Affinity Capture-MS Homo sapiens
26 ENTPD7  
Affinity Capture-MS Homo sapiens
27 BSG 682
Affinity Capture-MS Homo sapiens
28 COX2 4513
Co-fractionation Homo sapiens
29 CD1B 910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 C17orf78  
Affinity Capture-MS Homo sapiens
31 IFT27 11020
Affinity Capture-MS Homo sapiens
32 HCRTR2  
Affinity Capture-MS Homo sapiens
33 NR3C1 2908
Proximity Label-MS Homo sapiens
34 GCGR  
Affinity Capture-MS Homo sapiens
35 CLEC4A  
Affinity Capture-MS Homo sapiens
36 LGALS9B 284194
Affinity Capture-MS Homo sapiens
37 CALM1 801
Affinity Capture-MS Homo sapiens
38 PARK2  
Affinity Capture-MS Homo sapiens
39 HTR2C  
Two-hybrid Homo sapiens
40 OPRL1 4987
Affinity Capture-MS Homo sapiens
41 P2RY8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PRKAG1 5571
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MCAM 4162
Proximity Label-MS Homo sapiens
44 CD226 10666
Affinity Capture-MS Homo sapiens
45 CD27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 C5AR1  
Affinity Capture-MS Homo sapiens
47 CTDSP2 10106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 HM13 81502
Co-fractionation Homo sapiens
49 C3orf18  
Affinity Capture-MS Homo sapiens
50 IL2RA  
Affinity Capture-MS Homo sapiens
51 CYP2B6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 S1PR1 1901
Affinity Capture-MS Homo sapiens
53 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 FAM174A 345757
Affinity Capture-MS Homo sapiens
56 FAF2 23197
Affinity Capture-MS Homo sapiens
57 TPST2 8459
Affinity Capture-MS Homo sapiens
58 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 FOXR1  
Affinity Capture-MS Homo sapiens
60 PTGFR 5737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 NIPAL1  
Affinity Capture-MS Homo sapiens
62 OR13J1  
Affinity Capture-MS Homo sapiens
63 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 TNFRSF13C  
Affinity Capture-MS Homo sapiens
66 SLC26A4 5172
Affinity Capture-MS Homo sapiens
67 RANBP6 26953
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 AGTR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CSGALNACT1 55790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 OPALIN  
Affinity Capture-MS Homo sapiens
72 DGCR2 9993
Affinity Capture-MS Homo sapiens
73 AVPR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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