Gene description for TSPAN5
Gene name tetraspanin 5
Gene symbol TSPAN5
Other names/aliases NET-4
NET4
TM4SF9
TSPAN-5
Species Homo sapiens
 Database cross references - TSPAN5
ExoCarta ExoCarta_10098
Vesiclepedia VP_10098
Entrez Gene 10098
HGNC 17753
MIM 613136
UniProt P62079  
 TSPAN5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
 Gene ontology annotations for TSPAN5
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
Biological Process
    positive regulation of Notch signaling pathway GO:0045747 IMP
    regulation of membrane protein ectodomain proteolysis GO:0051043 IDA
    protein maturation GO:0051604 IMP
    protein localization to plasma membrane GO:0072659 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    actin cytoskeleton GO:0015629 IDA
 Experiment description of studies that identified TSPAN5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
 Protein-protein interactions for TSPAN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADAM10 102
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 ATP2B3 492
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DNAJC19 131118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SLC20A1 6574
Affinity Capture-MS Homo sapiens
5 GLUD2 2747
Affinity Capture-MS Homo sapiens
6 LCLAT1 253558
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SLC38A2 54407
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GPR89A  
Affinity Capture-MS Homo sapiens
9 RDH14 57665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PSEN1 5663
Affinity Capture-MS Homo sapiens
11 AGPAT4 56895
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SNX25 83891
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TMEM87A 25963
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
15 ZDHHC9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PIEZO1 9780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FAM210B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GP1BB 2812
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 B4GALT1 2683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TVP23C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SEC11A 23478
Affinity Capture-MS Homo sapiens
22 TMEM248 55069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 TMEM206 55248
Affinity Capture-MS Homo sapiens
24 RDH12 145226
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 FAM213A 84293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TMUB1  
Affinity Capture-MS Homo sapiens
28 CD47 961
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 BSCL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 TSPAN15 23555
Affinity Capture-MS Homo sapiens
31 EFNB1 1947
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TMEM186  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 GHDC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 THAP11 57215
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 IMMP1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AGPAT3 56894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 NAT14 57106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 APP 351
Reconstituted Complex Homo sapiens
39 KLHL17  
Affinity Capture-MS Homo sapiens
40 KEAP1 9817
Affinity Capture-MS Homo sapiens
41 SNX19  
Affinity Capture-MS Homo sapiens
42 KDSR 2531
Affinity Capture-MS Homo sapiens
43 TMEM43 79188
Affinity Capture-MS Homo sapiens
44 MAGT1 84061
Affinity Capture-MS Homo sapiens
45 UBAC2 337867
Affinity Capture-MS Homo sapiens
46 SOAT1 6646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ALG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RFT1 91869
Affinity Capture-MS Homo sapiens
50 ZDHHC6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SEC62 7095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NDC1 55706
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NTSR1  
Affinity Capture-MS Homo sapiens
54 NAT8L 339983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SCAMP2 10066
Affinity Capture-MS Homo sapiens
56 LEMD2 221496
Affinity Capture-MS Homo sapiens
57 CYB5R3 1727
Affinity Capture-MS Homo sapiens
58 SPCS1 28972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 RDH11 51109
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SLC7A2 6542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 DAGLB 221955
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 SLC27A2 11001
Affinity Capture-MS Homo sapiens
63 PIGS 94005
Affinity Capture-MS Homo sapiens
64 FTSJ2 29960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PRAF2 11230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RER1 11079
Affinity Capture-MS Homo sapiens
67 GDPD1 284161
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 ALG11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 WDR41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 BRI3BP 140707
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ZDHHC21  
Affinity Capture-MS Homo sapiens
72 SC5D 6309
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PSEN2 5664
Affinity Capture-MS Homo sapiens
74 SPCS2 9789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 TMEM192 201931
Affinity Capture-MS Homo sapiens
77 CELSR2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TSPAN5 is involved
PathwayEvidenceSource
Amyloid fiber formation TAS Reactome
Metabolism of proteins TAS Reactome





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