Gene description for NAT8L
Gene name N-acetyltransferase 8-like (GCN5-related, putative)
Gene symbol NAT8L
Other names/aliases CML3
NACED
NAT8-LIKE
Species Homo sapiens
 Database cross references - NAT8L
ExoCarta ExoCarta_339983
Vesiclepedia VP_339983
Entrez Gene 339983
HGNC 26742
MIM 610647
UniProt Q8N9F0  
 NAT8L identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NAT8L
Molecular Function
    protein binding GO:0005515 IPI
    aspartate N-acetyltransferase activity GO:0017188 IBA
    aspartate N-acetyltransferase activity GO:0017188 IDA
    aspartate N-acetyltransferase activity GO:0017188 IMP
    aspartate N-acetyltransferase activity GO:0017188 TAS
Biological Process
    aspartate family amino acid metabolic process GO:0009066 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    mitochondrial membrane GO:0031966 IBA
    mitochondrial membrane GO:0031966 IDA
 Experiment description of studies that identified NAT8L in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NAT8L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZDHHC12  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 KIAA0232 9778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 FLNC 2318
Affinity Capture-MS Homo sapiens
5 FAT3 120114
Affinity Capture-MS Homo sapiens
6 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 C3orf52  
Affinity Capture-MS Homo sapiens
8 TRPV3  
Affinity Capture-MS Homo sapiens
9 PEX19 5824
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NAT8L is involved
PathwayEvidenceSource
Aspartate and asparagine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here