Gene description for LCLAT1
Gene name lysocardiolipin acyltransferase 1
Gene symbol LCLAT1
Other names/aliases 1AGPAT8
AGPAT8
ALCAT1
HSRG1849
LYCAT
UNQ1849
Species Homo sapiens
 Database cross references - LCLAT1
ExoCarta ExoCarta_253558
Vesiclepedia VP_253558
Entrez Gene 253558
HGNC 26756
MIM 614241
UniProt Q6UWP7  
 LCLAT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LCLAT1
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IDA
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    protein binding GO:0005515 IPI
    O-acyltransferase activity GO:0008374 TAS
    acyltransferase activity GO:0016746 IBA
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
    cardiolipin acyl-chain remodeling GO:0035965 TAS
    phosphatidylinositol acyl-chain remodeling GO:0036149 IBA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 IDA
    endomembrane system GO:0012505 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified LCLAT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LCLAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NKAIN1  
Affinity Capture-MS Homo sapiens
2 ADAM30 11085
Affinity Capture-MS Homo sapiens
3 ADAM2  
Affinity Capture-MS Homo sapiens
4 KCNMB3  
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 IGFL3  
Affinity Capture-MS Homo sapiens
8 SLC30A2  
Affinity Capture-MS Homo sapiens
9 KCNA2  
Affinity Capture-MS Homo sapiens
10 TTYH1  
Affinity Capture-MS Homo sapiens
11 RXFP1  
Affinity Capture-MS Homo sapiens
12 SIDT2 51092
Affinity Capture-MS Homo sapiens
13 TP53 7157
Affinity Capture-MS Homo sapiens
14 TM4SF5  
Affinity Capture-MS Homo sapiens
15 FOLR1 2348
Affinity Capture-MS Homo sapiens
16 PGRMC1 10857
Affinity Capture-MS Homo sapiens
17 FRMD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CLEC2D  
Affinity Capture-MS Homo sapiens
20 SLC39A7 7922
Affinity Capture-MS Homo sapiens
21 ADAM32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TSPAN15 23555
Affinity Capture-MS Homo sapiens
23 COPB2 9276
Affinity Capture-MS Homo sapiens
24 NIPAL1  
Affinity Capture-MS Homo sapiens
25 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
26 PRRT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 DNAJC5 80331
Affinity Capture-MS Homo sapiens
28 GPR114  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TMEM72  
Affinity Capture-MS Homo sapiens
30 COPE 11316
Affinity Capture-MS Homo sapiens
31 FAM174A 345757
Affinity Capture-MS Homo sapiens
32 COPA 1314
Affinity Capture-MS Homo sapiens
33 TMEM63C  
Affinity Capture-MS Homo sapiens
34 MS4A15  
Affinity Capture-MS Homo sapiens
35 GPR52  
Affinity Capture-MS Homo sapiens
36 TSPAN2 10100
Affinity Capture-MS Homo sapiens
37 KIAA1429 25962
Affinity Capture-MS Homo sapiens
38 GABRG2  
Affinity Capture-MS Homo sapiens
39 LPCAT2 54947
Affinity Capture-MS Homo sapiens
40 ADAM21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TSPAN8 7103
Affinity Capture-MS Homo sapiens
42 CHRNA9  
Affinity Capture-MS Homo sapiens
43 FAM134C 162427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 C3orf52  
Affinity Capture-MS Homo sapiens
45 IPPK  
Affinity Capture-MS Homo sapiens
46 CMTM5  
Affinity Capture-MS Homo sapiens
47 ATP2B2 491
Affinity Capture-MS Homo sapiens
48 GPR21  
Affinity Capture-MS Homo sapiens
49 SLC4A8 9498
Affinity Capture-MS Homo sapiens
50 MARCH4  
Affinity Capture-MS Homo sapiens
51 PVRIG  
Affinity Capture-MS Homo sapiens
52 OR10H2  
Affinity Capture-MS Homo sapiens
53 LYPD6  
Affinity Capture-MS Homo sapiens
54 RNF2  
Affinity Capture-MS Homo sapiens
55 CCR6  
Affinity Capture-MS Homo sapiens
56 CD63 967
Affinity Capture-MS Homo sapiens
57 LMNA 4000
Proximity Label-MS Homo sapiens
58 RPA3 6119
Proximity Label-MS Homo sapiens
59 LNX1  
Two-hybrid Homo sapiens
60 GOPC 57120
Two-hybrid Homo sapiens
61 CHRND  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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