Experiment description of studies that identified TSPAN8 in exosomes

1 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

2 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

3 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 282 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

7 Experiment ID 283 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Ultracentrifugation - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

8 Experiment ID 285 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

9 Experiment ID 286 MISEV standards ✔ CEM Biophysical techniques ✔ Alix|TSG101|CD63|CD81|EpCAM Enriched markers ✘ Negative markers ✔ DLS Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25890246     Organism Homo sapiens Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ." Journal name Methods Publication year 2015 Sample Colorectal cancer cells Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 Isolation/purification methods Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

10 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

Protein-protein interactions for TSPAN8

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HAL 3034 Affinity Capture-MS Homo sapiens 2 ZDHHC20 253832 Affinity Capture-MS Homo sapiens 3 PIGT 51604 Affinity Capture-MS Homo sapiens 4 TMEM246 84302 Affinity Capture-MS Homo sapiens 5 SLC35E1 79939 Affinity Capture-MS Homo sapiens 6 ARV1   Affinity Capture-MS Homo sapiens 7 LCLAT1 253558 Affinity Capture-MS Homo sapiens 8 C4orf32   Affinity Capture-MS Homo sapiens 9 SEC61A2 55176 Affinity Capture-MS Homo sapiens 10 GPAA1 8733 Affinity Capture-MS Homo sapiens 11 AGPAT4 56895 Affinity Capture-MS Homo sapiens 12 TMEM87A 25963 Affinity Capture-MS Homo sapiens 13 DGAT1 8694 Affinity Capture-MS Homo sapiens 14 USP34 9736 Affinity Capture-MS Homo sapiens 15 FITM2   Affinity Capture-MS Homo sapiens 16 PCYOX1 51449 Affinity Capture-MS Homo sapiens 17 TMEM68   Affinity Capture-MS Homo sapiens 18 TELO2 9894 Affinity Capture-MS Homo sapiens 19 PXMP2   Affinity Capture-MS Homo sapiens 20 TMEM63B 55362 Affinity Capture-MS Homo sapiens 21 C10orf35   Affinity Capture-MS Homo sapiens 22 POF1B 79983 Affinity Capture-MS Homo sapiens 23 MGAT2 4247 Affinity Capture-MS Homo sapiens 24 SCAMP3 10067 Affinity Capture-MS Homo sapiens 25 PNLIPRP1   Two-hybrid Homo sapiens 26 TMEM186   Affinity Capture-MS Homo sapiens 27 TMEM161B   Affinity Capture-MS Homo sapiens 28 UNC93B1 81622 Affinity Capture-MS Homo sapiens 29 REEP4   Affinity Capture-MS Homo sapiens 30 CIB1 10519 Affinity Capture-MS Homo sapiens 31 AGPAT3 56894 Affinity Capture-MS Homo sapiens 32 ARL6IP1 23204 Affinity Capture-MS Homo sapiens 33 VAC14 55697 Affinity Capture-MS Homo sapiens 34 LPCAT3 10162 Affinity Capture-MS Homo sapiens 35 CYFIP2 26999 Affinity Capture-MS Homo sapiens 36 SLC38A7 55238 Two-hybrid Homo sapiens 37 CPT1A 1374 Affinity Capture-MS Homo sapiens 38 SLC19A2 10560 Affinity Capture-MS Homo sapiens 39 KDELR1 10945 Affinity Capture-MS Homo sapiens 40 POTEF 728378 Affinity Capture-MS Homo sapiens 41 SNX19   Affinity Capture-MS Homo sapiens 42 CNNM3 26505 Affinity Capture-MS Homo sapiens 43 TMEM43 79188 Affinity Capture-MS Homo sapiens 44 SHH   Affinity Capture-Western Homo sapiens 45 FAM134C 162427 Affinity Capture-MS Homo sapiens 46 SLC5A3 6526 Affinity Capture-MS Homo sapiens 47 IST1 9798 Affinity Capture-MS Homo sapiens 48 TP53I11   Affinity Capture-MS Homo sapiens 49 LMAN2L 81562 Affinity Capture-MS Homo sapiens 50 TMEM194A   Affinity Capture-MS Homo sapiens 51 SLC30A7 148867 Affinity Capture-MS Homo sapiens 52 SLC25A52   Affinity Capture-MS Homo sapiens 53 AGPAT6 137964 Affinity Capture-MS Homo sapiens 54 MPDU1 9526 Affinity Capture-MS Homo sapiens 55 TIMM23 100287932 Two-hybrid Homo sapiens 56 LETMD1   Affinity Capture-MS Homo sapiens 57 TAP1 6890 Affinity Capture-MS Homo sapiens 58 PIGS 94005 Affinity Capture-MS Homo sapiens 59 SLC30A3   Affinity Capture-MS Homo sapiens 60 ATP9B 374868 Affinity Capture-MS Homo sapiens 61 RER1 11079 Affinity Capture-MS Homo sapiens 62 SERPINA12   Affinity Capture-MS Homo sapiens 63 PTCH1   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 64 ALG11   Affinity Capture-MS Homo sapiens 65 SLC25A17 10478 Affinity Capture-MS Homo sapiens 66 XPO7 23039 Affinity Capture-MS Homo sapiens 67 ABCB7 22 Affinity Capture-MS Homo sapiens 68 HSDL1 83693 Affinity Capture-MS Homo sapiens 69 TMCO3 55002 Affinity Capture-MS Homo sapiens 70 NSUN5   Affinity Capture-MS Homo sapiens 71 FAM210B   Affinity Capture-MS Homo sapiens 72 STX2 2054 Two-hybrid Homo sapiens View the network

Pathways in which TSPAN8 is involved

No pathways found