Gene description for AGPAT4
Gene name 1-acylglycerol-3-phosphate O-acyltransferase 4
Gene symbol AGPAT4
Other names/aliases 1-AGPAT4
LPAAT-delta
dJ473J16.2
Species Homo sapiens
 Database cross references - AGPAT4
ExoCarta ExoCarta_56895
Vesiclepedia VP_56895
Entrez Gene 56895
HGNC 20885
MIM 614795
UniProt Q9NRZ5  
 AGPAT4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for AGPAT4
Molecular Function
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 IBA
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 ISS
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 NAS
    1-acylglycerol-3-phosphate O-acyltransferase activity GO:0003841 TAS
    protein binding GO:0005515 IPI
Biological Process
    phosphatidic acid biosynthetic process GO:0006654 TAS
    phospholipid biosynthetic process GO:0008654 NAS
    CDP-diacylglycerol biosynthetic process GO:0016024 IEA
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IBA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    endomembrane system GO:0012505 IBA
 Experiment description of studies that identified AGPAT4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AGPAT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GRPR  
Affinity Capture-MS Homo sapiens
2 REEP5 7905
Affinity Capture-MS Homo sapiens
3 RAMP3  
Affinity Capture-MS Homo sapiens
4 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 VIPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 ATP13A3 79572
Affinity Capture-MS Homo sapiens
7 CXCR4 7852
Affinity Capture-MS Homo sapiens
8 TTYH1  
Affinity Capture-MS Homo sapiens
9 TSPAN15 23555
Affinity Capture-MS Homo sapiens
10 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LDLRAD1  
Affinity Capture-MS Homo sapiens
12 REL 5966
Two-hybrid Homo sapiens
13 CLEC2D  
Affinity Capture-MS Homo sapiens
14 SLC39A4 55630
Affinity Capture-MS Homo sapiens
15 IGHM 3507
Affinity Capture-MS Homo sapiens
16 TSPAN17 26262
Affinity Capture-MS Homo sapiens
17 GCGR  
Affinity Capture-MS Homo sapiens
18 NIPAL1  
Affinity Capture-MS Homo sapiens
19 DDX58 23586
Affinity Capture-RNA Homo sapiens
20 LPAR1 1902
Affinity Capture-MS Homo sapiens
21 SCN4A  
Affinity Capture-MS Homo sapiens
22 TSPAN8 7103
Affinity Capture-MS Homo sapiens
23 CYB5A 1528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SEC62 7095
Affinity Capture-MS Homo sapiens
25 CMTM5  
Affinity Capture-MS Homo sapiens
26 VIPR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CACNG1  
Affinity Capture-MS Homo sapiens
28 SLC22A4 6583
Affinity Capture-MS Homo sapiens
29 OR1D4  
Affinity Capture-MS Homo sapiens
30 OPALIN  
Affinity Capture-MS Homo sapiens
31 SLC7A1 6541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TSPAN10 83882
Affinity Capture-MS Homo sapiens
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