Gene description for CYB5A
Gene name cytochrome b5 type A (microsomal)
Gene symbol CYB5A
Other names/aliases CYB5
MCB5
Species Homo sapiens
 Database cross references - CYB5A
ExoCarta ExoCarta_1528
Entrez Gene 1528
HGNC 2570
MIM 613218
UniProt P00167  
 CYB5A identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for CYB5A
Molecular Function
    heme binding GO:0020037 IEA
    aldo-keto reductase (NADP) activity GO:0004033 TAS
    metal ion binding GO:0046872 IEA
    cytochrome-c oxidase activity GO:0004129 TAS
    enzyme binding GO:0019899 IPI
Biological Process
    hydrogen ion transmembrane transport GO:1902600 TAS
    small molecule metabolic process GO:0044281 TAS
    L-ascorbic acid metabolic process GO:0019852 TAS
    oxidation-reduction process GO:0055114 IEA
    vitamin metabolic process GO:0006766 TAS
    water-soluble vitamin metabolic process GO:0006767 TAS
Subcellular Localization
    mitochondrial outer membrane GO:0005741 TAS
    membrane GO:0016020 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified CYB5A in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CYB5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SCD 6319
Invitro Homo sapiens
2 CYP1A1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
3 CYCS  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 CYP17A1 1586
Invitro Homo sapiens
5 CYB5R3 1727
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which CYB5A is involved
PathwayEvidenceSource
Vitamin C (ascorbate) metabolism TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here