Gene description for CYB5A
Gene name cytochrome b5 type A (microsomal)
Gene symbol CYB5A
Other names/aliases CYB5
MCB5
Species Homo sapiens
 Database cross references - CYB5A
ExoCarta ExoCarta_1528
Vesiclepedia VP_1528
Entrez Gene 1528
HGNC 2570
MIM 613218
UniProt P00167  
 CYB5A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CYB5A
Molecular Function
    cytochrome-c oxidase activity GO:0004129 TAS
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    heme binding GO:0020037 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrial outer membrane GO:0005741 TAS
    endoplasmic reticulum membrane GO:0005789 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified CYB5A in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CYB5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Co-fractionation Homo sapiens
2 COX5A 9377
Co-fractionation Homo sapiens
3 GJD3  
Proximity Label-MS Homo sapiens
4 REEP5 7905
Proximity Label-MS Homo sapiens
5 CYP1A2  
Reconstituted Complex Homo sapiens
6 METTL7A 25840
Proximity Label-MS Homo sapiens
7 TPP1 1200
Co-fractionation Homo sapiens
8 SLC38A6  
Affinity Capture-MS Homo sapiens
9 SRPR 6734
Affinity Capture-MS Homo sapiens
10 TAB1 10454
Affinity Capture-MS Homo sapiens
11 SYVN1 84447
Affinity Capture-MS Homo sapiens
12 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RTN4 57142
Affinity Capture-MS Homo sapiens
14 SOX2  
Affinity Capture-MS Homo sapiens
15 HSPA5 3309
Co-fractionation Homo sapiens
16 NDUFB9 4715
Co-fractionation Homo sapiens
17 B3GAT1  
Proximity Label-MS Homo sapiens
18 CORO1B 57175
Co-fractionation Homo sapiens
19 ISYNA1 51477
Co-fractionation Homo sapiens
20 NDUFA7 4701
Co-fractionation Homo sapiens
21 COPB2 9276
Affinity Capture-MS Homo sapiens
22 RCN2 5955
Co-fractionation Homo sapiens
23 SEC24C 9632
Co-fractionation Homo sapiens
24 NDUFA5 4698
Co-fractionation Homo sapiens
25 COA3 28958
Co-fractionation Homo sapiens
26 SSR1 6745
Proximity Label-MS Homo sapiens
27 RER1 11079
Affinity Capture-MS Homo sapiens
28 DDOST 1650
Affinity Capture-MS Homo sapiens
29 ATP5O 539
Co-fractionation Homo sapiens
30 CYP2D6 1565
Reconstituted Complex Homo sapiens
31 CCDC47 57003
Affinity Capture-MS Homo sapiens
32 PHB 5245
Co-fractionation Homo sapiens
33 MXRA7 439921
Co-fractionation Homo sapiens
34 UGT1A4  
Two-hybrid Homo sapiens
35 ATP5H 10476
Co-fractionation Homo sapiens
36 AGPAT4 56895
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MLEC 9761
Co-fractionation Homo sapiens
38 CYBRD1 79901
Co-fractionation Homo sapiens
39 LAMTOR1 55004
Co-fractionation Homo sapiens
40 FAS 355
Proximity Label-MS Homo sapiens
41 NDUFB1 4707
Co-fractionation Homo sapiens
42 KIAA1715 80856
Proximity Label-MS Homo sapiens
43 ATP6V1G1 9550
Co-fractionation Homo sapiens
44 CYP2W1  
Two-hybrid Homo sapiens
45 MTCH2 23788
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
46 NDUFS4 4724
Co-fractionation Homo sapiens
47 EMD 2010
Proximity Label-MS Homo sapiens
48 TMX1 81542
Co-fractionation Homo sapiens
49 CXADR 1525
Proximity Label-MS Homo sapiens
50 OR10H3  
Affinity Capture-MS Homo sapiens
51 SYPL2  
Affinity Capture-MS Homo sapiens
52 UCHL5 51377
Affinity Capture-MS Homo sapiens
53 KDELC2 143888
Co-fractionation Homo sapiens
54 SSUH2  
Affinity Capture-MS Homo sapiens
55 RPN1 6184
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
56 ELOVL5 60481
Proximity Label-MS Homo sapiens
57 SEC61B 10952
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
58 GPRC5A 9052
Co-fractionation Homo sapiens
59 ETHE1 23474
Co-fractionation Homo sapiens
60 SLC25A24 29957
Co-fractionation Homo sapiens
61 CYP2A6  
Reconstituted Complex Homo sapiens
62 C4orf32  
Affinity Capture-MS Homo sapiens
63 CYP3A4  
Reconstituted Complex Homo sapiens
64 RPN2 6185
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CANX 821
Affinity Capture-MS Homo sapiens
66 AGPAT1 10554
Affinity Capture-MS Homo sapiens
67 LRRC59 55379
Proximity Label-MS Homo sapiens
68 PANX1 24145
Proximity Label-MS Homo sapiens
69 BCAP31 10134
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
70 HSD3B7 80270
Proximity Label-MS Homo sapiens
71 ENDOD1 23052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 HSPA14 51182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 STIM1 6786
Proximity Label-MS Homo sapiens
74 EGFR 1956
Negative Genetic Homo sapiens
75 OCIAD1 54940
Proximity Label-MS Homo sapiens
76 PRKCSH 5589
Co-fractionation Homo sapiens
77 ARHGAP15 55843
Affinity Capture-MS Homo sapiens
78 NDUFB3 4709
Co-fractionation Homo sapiens
79 COX6C 1345
Co-fractionation Homo sapiens
80 Cyp1a2  
Reconstituted Complex Rattus norvegicus
81 FYTTD1  
Affinity Capture-MS Homo sapiens
82 LMNA 4000
Proximity Label-MS Homo sapiens
83 HSD17B12 51144
Co-fractionation Homo sapiens
84 AKAP1 8165
Proximity Label-MS Homo sapiens
85 TMX2 51075
Co-fractionation Homo sapiens
86 OST4  
Affinity Capture-MS Homo sapiens
87 GBAS 2631
Co-fractionation Homo sapiens
88 PHB2 11331
Co-fractionation Homo sapiens
89 TMED10 10972
Affinity Capture-MS Homo sapiens
90 SOD2 6648
Co-fractionation Homo sapiens
91 PGRMC1 10857
Co-fractionation Homo sapiens
92 HSD17B11 51170
Proximity Label-MS Homo sapiens
93 EMC4 51234
Affinity Capture-MS Homo sapiens
94 NUP35 129401
Proximity Label-MS Homo sapiens
95 WIF1 11197
Affinity Capture-MS Homo sapiens
96 GNG12 55970
Co-fractionation Homo sapiens
97 MBNL1 4154
Affinity Capture-MS Homo sapiens
98 ATP6V0C 527
Co-fractionation Homo sapiens
99 LRPAP1 4043
Co-fractionation Homo sapiens
100 SEC62 7095
Proximity Label-MS Homo sapiens
101 CYP2E1  
Reconstituted Complex Homo sapiens
102 DHFRL1  
Proximity Label-MS Homo sapiens
103 C1QBP 708
Co-fractionation Homo sapiens
104 SNX2 6643
Affinity Capture-MS Homo sapiens
105 KRAS 3845
Negative Genetic Homo sapiens
106 COX7C 1350
Co-fractionation Homo sapiens
107 CYCS 54205
Reconstituted Complex Homo sapiens
108 CKAP4 10970
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
109 CCR1  
Affinity Capture-MS Homo sapiens
110 KCNMB1  
Co-fractionation Homo sapiens
111 CUL4A 8451
Affinity Capture-MS Homo sapiens
112 DERL1 79139
Proximity Label-MS Homo sapiens
113 ATP2A1 487
Proximity Label-MS Homo sapiens
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