Gene description for SOD2
Gene name superoxide dismutase 2, mitochondrial
Gene symbol SOD2
Other names/aliases IPOB
MNSOD
MVCD6
Species Homo sapiens
 Database cross references - SOD2
ExoCarta ExoCarta_6648
Vesiclepedia VP_6648
Entrez Gene 6648
HGNC 11180
MIM 147460
UniProt P04179  
 SOD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SOD2
Molecular Function
    superoxide dismutase activity GO:0004784 TAS
    manganese ion binding GO:0030145 TAS
    oxygen binding GO:0019825 IEA
    identical protein binding GO:0042802 IPI
    DNA binding GO:0003677 IEA
Biological Process
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IEA
    negative regulation of neuron apoptotic process GO:0043524 IGI
    vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure GO:0003069 ISS
    respiratory electron transport chain GO:0022904 IEA
    regulation of mitochondrial membrane potential GO:0051881 IEA
    release of cytochrome c from mitochondria GO:0001836 ISS
    glutathione metabolic process GO:0006749 IEA
    superoxide metabolic process GO:0006801 IDA
    detection of oxygen GO:0003032 IEA
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    response to silicon dioxide GO:0034021 IEA
    response to hydrogen peroxide GO:0042542 IEA
    erythrophore differentiation GO:0048773 IEA
    hemopoiesis GO:0030097 IEA
    locomotory behavior GO:0007626 IEA
    response to manganese ion GO:0010042 IEA
    response to superoxide GO:0000303 IMP
    response to hyperoxia GO:0055093 IEA
    response to drug GO:0042493 IEA
    response to electrical stimulus GO:0051602 IEA
    removal of superoxide radicals GO:0019430 IMP
    response to activity GO:0014823 IEA
    response to lipopolysaccharide GO:0032496 IEA
    response to magnetism GO:0071000 IEA
    hydrogen peroxide biosynthetic process GO:0050665 IEA
    negative regulation of cell proliferation GO:0008285 IMP
    cellular response to ethanol GO:0071361 IEA
    regulation of blood pressure GO:0008217 ISS
    post-embryonic development GO:0009791 IEA
    neuron development GO:0048666 IEA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IMP
    negative regulation of fat cell differentiation GO:0045599 IEA
    response to cold GO:0009409 IEA
    liver development GO:0001889 IEA
    heart development GO:0007507 IEA
    response to L-ascorbic acid GO:0033591 IEA
    response to gamma radiation GO:0010332 IEA
    age-dependent response to reactive oxygen species GO:0001315 IMP
    response to cadmium ion GO:0046686 IEA
    protein homotetramerization GO:0051289 IPI
    response to isolation stress GO:0035900 IEA
    negative regulation of fibroblast proliferation GO:0048147 IEA
    iron ion homeostasis GO:0055072 IEA
    response to zinc ion GO:0010043 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 IMP
    response to hypoxia GO:0001666 IEA
    response to selenium ion GO:0010269 IEA
    superoxide anion generation GO:0042554 IEA
    response to reactive oxygen species GO:0000302 TAS
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    response to axon injury GO:0048678 IEA
    oxygen homeostasis GO:0032364 IMP
    response to immobilization stress GO:0035902 IEA
    regulation of catalytic activity GO:0050790 IEA
Subcellular Localization
    mitochondrial matrix GO:0005759 TAS
    mitochondrial nucleoid GO:0042645 IEA
    mitochondrion GO:0005739 IDA
    extracellular exosome GO:0070062 IDA
    mitochondrial inner membrane GO:0005743 IEA
 Experiment description of studies that identified SOD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD.
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SOD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SOD2 6648
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NOL12 79159
Two-hybrid Homo sapiens
3 AURKA 6790
Affinity Capture-MS Homo sapiens
4 RPS3A 6189
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SOD2 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species TAS Reactome





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