Gene description for SOD2
Gene name superoxide dismutase 2, mitochondrial
Gene symbol SOD2
Other names/aliases IPOB
MNSOD
MVCD6
Species Homo sapiens
 Database cross references - SOD2
ExoCarta ExoCarta_6648
Vesiclepedia VP_6648
Entrez Gene 6648
HGNC 11180
MIM 147460
UniProt P04179  
 SOD2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SOD2
Molecular Function
    DNA binding GO:0003677 IEA
    superoxide dismutase activity GO:0004784 IBA
    superoxide dismutase activity GO:0004784 IDA
    protein binding GO:0005515 IPI
    oxygen binding GO:0019825 IEA
    enzyme binding GO:0019899 IEA
    manganese ion binding GO:0030145 IBA
    manganese ion binding GO:0030145 IDA
    manganese ion binding GO:0030145 TAS
    identical protein binding GO:0042802 IPI
Biological Process
    response to superoxide GO:0000303 IMP
    response to hypoxia GO:0001666 IEA
    release of cytochrome c from mitochondria GO:0001836 ISS
    liver development GO:0001889 IEA
    detection of oxygen GO:0003032 IEA
    acetylcholine-mediated vasodilation involved in regulation of systemic arterial blood pressure GO:0003069 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IMP
    glutathione metabolic process GO:0006749 IEA
    superoxide metabolic process GO:0006801 IDA
    heart development GO:0007507 IEA
    locomotory behavior GO:0007626 IEA
    regulation of blood pressure GO:0008217 ISS
    negative regulation of cell population proliferation GO:0008285 IMP
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IEA
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    post-embryonic development GO:0009791 IEA
    response to manganese ion GO:0010042 IEA
    response to zinc ion GO:0010043 IEA
    response to selenium ion GO:0010269 IEA
    response to gamma radiation GO:0010332 IEA
    positive regulation of hydrogen peroxide biosynthetic process GO:0010729 IEA
    response to activity GO:0014823 IEA
    removal of superoxide radicals GO:0019430 IMP
    respiratory electron transport chain GO:0022904 IEA
    hemopoiesis GO:0030097 IEA
    positive regulation of cell migration GO:0030335 IMP
    intracellular oxygen homeostasis GO:0032364 IMP
    response to lipopolysaccharide GO:0032496 IEA
    response to L-ascorbic acid GO:0033591 IEA
    response to silicon dioxide GO:0034021 IEA
    cellular response to oxidative stress GO:0034599 IMP
    response to isolation stress GO:0035900 IEA
    response to immobilization stress GO:0035902 IEA
    response to hydrogen peroxide GO:0042542 IEA
    superoxide anion generation GO:0042554 IEA
    negative regulation of neuron apoptotic process GO:0043524 IGI
    positive regulation of nitric oxide biosynthetic process GO:0045429 IEA
    negative regulation of fat cell differentiation GO:0045599 IEA
    response to cadmium ion GO:0046686 IEA
    negative regulation of fibroblast proliferation GO:0048147 IEA
    neuron development GO:0048666 IEA
    response to axon injury GO:0048678 IEA
    erythrophore differentiation GO:0048773 IEA
    hydrogen peroxide biosynthetic process GO:0050665 IEA
    protein homotetramerization GO:0051289 IPI
    response to electrical stimulus GO:0051602 IEA
    regulation of mitochondrial membrane potential GO:0051881 IEA
    response to hyperoxia GO:0055093 IEA
    multicellular organismal-level iron ion homeostasis GO:0060586 IEA
    response to magnetism GO:0071000 IEA
    cellular response to ethanol GO:0071361 IEA
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IMP
    negative regulation of membrane hyperpolarization GO:1902631 IEA
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IDA
    positive regulation of vascular associated smooth muscle cell apoptotic process GO:1905461 IDA
    positive regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching GO:1905932 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    mitochondrial nucleoid GO:0042645 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SOD2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SOD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ENO3 2027
Co-fractionation Homo sapiens
2 UBL4A 8266
Co-fractionation Homo sapiens
3 RPS3A 6189
Two-hybrid Homo sapiens
4 VAPA 9218
Co-fractionation Homo sapiens
5 PKM 5315
Co-fractionation Homo sapiens
6 CYP4F12  
Two-hybrid Homo sapiens
7 ALDH2 217
Co-fractionation Homo sapiens
8 NABP2  
Co-fractionation Homo sapiens
9 GPT2  
Co-fractionation Homo sapiens
10 PPIA 5478
Co-fractionation Homo sapiens
11 PIR 8544
Co-fractionation Homo sapiens
12 PEBP1 5037
Co-fractionation Homo sapiens
13 PARK7 11315
Co-fractionation Homo sapiens
14 ALDH7A1 501
Co-fractionation Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 CMBL 134147
Co-fractionation Homo sapiens
17 USP36  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 CEP70  
Two-hybrid Homo sapiens
19 AK3 50808
Co-fractionation Homo sapiens
20 STX7 8417
Co-fractionation Homo sapiens
21 MDH1 4190
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
22 DCTD 1635
Co-fractionation Homo sapiens
23 PEPD 5184
Co-fractionation Homo sapiens
24 SOD1 6647
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
25 ABAT  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
26 NFU1 27247
Co-fractionation Homo sapiens
27 E2F4  
Affinity Capture-MS Homo sapiens
28 AURKA 6790
Affinity Capture-MS Homo sapiens
29 DLD 1738
Co-fractionation Homo sapiens
30 PPIB 5479
Affinity Capture-MS Homo sapiens
31 PRDX5 25824
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
32 SUCLG1 8802
Co-fractionation Homo sapiens
33 ALDH5A1 7915
Co-fractionation Homo sapiens
34 TBL2 26608
Co-fractionation Homo sapiens
35 HRSP12 10247
Co-fractionation Homo sapiens
36 TRIM24  
Affinity Capture-MS Homo sapiens
37 ACACA 31
Positive Genetic Homo sapiens
38 C12orf49  
Positive Genetic Homo sapiens
39 KDELC2 143888
Co-fractionation Homo sapiens
40 MYDGF 56005
Co-fractionation Homo sapiens
41 TPI1 7167
Co-fractionation Homo sapiens
42 FH 2271
Co-fractionation Homo sapiens
43 PPA2 27068
Co-fractionation Homo sapiens
44 CYB5A 1528
Co-fractionation Homo sapiens
45 CTSB 1508
Co-fractionation Homo sapiens
46 FAHD2A 51011
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
47 UBR2 23304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CAT 847
Co-fractionation Homo sapiens
49 ADH5 128
Co-fractionation Homo sapiens
50 HTRA2 27429
Co-fractionation Homo sapiens
51 PITRM1 10531
Co-fractionation Homo sapiens
52 ANXA1 301
Co-fractionation Homo sapiens
53 SREBF1 6720
Positive Genetic Homo sapiens
54 EGFR 1956
Negative Genetic Homo sapiens
55 TST 7263
Co-fractionation Homo sapiens
56 ECI1 1632
Co-fractionation Homo sapiens
57 ZC3H11A  
Co-fractionation Homo sapiens
58 NOL12 79159
Two-hybrid Homo sapiens
59 CFL1 1072
Co-fractionation Homo sapiens
60 CTSS 1520
Co-fractionation Homo sapiens
61 CFL2 1073
Co-fractionation Homo sapiens
62 DSTN 11034
Co-fractionation Homo sapiens
63 H2AFX 3014
Affinity Capture-MS Homo sapiens
64 NIT2 56954
Co-fractionation Homo sapiens
65 HSPE1 3336
Co-fractionation Homo sapiens
66 CS 1431
Co-fractionation Homo sapiens
67 FHL1 2273
Co-fractionation Homo sapiens
68 ALDOC 230
Co-fractionation Homo sapiens
69 SOD2 6648
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
70 ALDH4A1 8659
Co-fractionation Homo sapiens
71 CUTA 51596
Co-fractionation Homo sapiens
72 HMGB1 3146
Affinity Capture-MS Homo sapiens
73 ABHD10 55347
Co-fractionation Homo sapiens
74 LGALS3 3958
Co-fractionation Homo sapiens
75 ALDH1B1 219
Co-fractionation Homo sapiens
76 ACAA2 10449
Co-fractionation Homo sapiens
77 GPX4 2879
Co-fractionation Homo sapiens
78 MT1X 4501
Co-fractionation Homo sapiens
79 TKT 7086
Co-fractionation Homo sapiens
80 SUCLA2 8803
Co-fractionation Homo sapiens
81 UCHL3 7347
Co-fractionation Homo sapiens
82 OAT 4942
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
83 IDH2 3418
Co-fractionation Homo sapiens
84 ISOC2  
Co-fractionation Homo sapiens
85 ACO2 50
Co-fractionation Homo sapiens
86 REXO2 25996
Co-fractionation Homo sapiens
87 LDLR 3949
Positive Genetic Homo sapiens
88 ALDOA 226
Co-fractionation Homo sapiens
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