Gene description for GPX4
Gene name glutathione peroxidase 4
Gene symbol GPX4
Other names/aliases GPx-4
GSHPx-4
MCSP
PHGPx
snGPx
snPHGPx
Species Homo sapiens
 Database cross references - GPX4
ExoCarta ExoCarta_2879
Vesiclepedia VP_2879
Entrez Gene 2879
HGNC 4556
MIM 138322
UniProt P36969  
 GPX4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for GPX4
Molecular Function
    glutathione peroxidase activity GO:0004602 IBA
    glutathione peroxidase activity GO:0004602 IDA
    glutathione peroxidase activity GO:0004602 IMP
    protein binding GO:0005515 IPI
    selenium binding GO:0008430 IEA
    identical protein binding GO:0042802 IMP
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 IBA
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 IDA
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 ISS
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 TAS
Biological Process
    chromatin organization GO:0006325 IEA
    phospholipid metabolic process GO:0006644 TAS
    apoptotic process GO:0006915 IEA
    response to oxidative stress GO:0006979 ISS
    spermatogenesis GO:0007283 ISS
    dendrite development GO:0016358 IEA
    arachidonate metabolic process GO:0019369 IDA
    lipoxygenase pathway GO:0019372 IDA
    cerebellum development GO:0021549 IEA
    response to estradiol GO:0032355 IEA
    response to lipopolysaccharide GO:0032496 IEA
    multicellular organism growth GO:0035264 IEA
    long-chain fatty acid biosynthetic process GO:0042759 TAS
    protein polymerization GO:0051258 IMP
    cellular oxidant detoxification GO:0098869 IEA
    negative regulation of ferroptosis GO:0110076 ISS
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nuclear envelope GO:0005635 IEA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IMP
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GPX4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
10
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
11
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GPX4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 TAGLN 6876
Co-fractionation Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 GSTO2 119391
Affinity Capture-MS Homo sapiens
5 VCY  
Affinity Capture-MS Homo sapiens
6 OTUD5 55593
Affinity Capture-MS Homo sapiens
7 UBE2H 7328
Affinity Capture-MS Homo sapiens
8 IMPA2 3613
Co-fractionation Homo sapiens
9 IL18 3606
Affinity Capture-MS Homo sapiens
10 NUTF2 10204
Co-fractionation Homo sapiens
11 SOD2 6648
Co-fractionation Homo sapiens
12 RAB35 11021
Co-fractionation Homo sapiens
13 AKR1B1 231
Co-fractionation Homo sapiens
14 SOD1 6647
Co-fractionation Homo sapiens
15 HNRNPA0 10949
Co-fractionation Homo sapiens
16 OMP  
Affinity Capture-MS Homo sapiens
17 CDA 978
Affinity Capture-MS Homo sapiens
18 HSPE1 3336
Co-fractionation Homo sapiens
19 PIN1 5300
Co-fractionation Homo sapiens
20 LHFPL4  
Affinity Capture-MS Homo sapiens
21 EZR 7430
Co-fractionation Homo sapiens
22 MAPK13 5603
Two-hybrid Homo sapiens
23 ATP5A1 498
Co-fractionation Homo sapiens
24 STIP1 10963
Co-fractionation Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 ETFB 2109
Co-fractionation Homo sapiens
27 PGAM4 441531
Co-fractionation Homo sapiens
28 HSD17B10 3028
Co-fractionation Homo sapiens
29 TKT 7086
Co-fractionation Homo sapiens
30 DUT 1854
Co-fractionation Homo sapiens
31 OTUB1 55611
Affinity Capture-MS Homo sapiens
32 FKBP1A 2280
Co-fractionation Homo sapiens
33 UCHL3 7347
Co-fractionation Homo sapiens
34 ENO3 2027
Co-fractionation Homo sapiens
35 PLOD1 5351
Co-fractionation Homo sapiens
36 ERO1LB 56605
Co-fractionation Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 SNRNP48  
Two-hybrid Homo sapiens
39 RPE  
Affinity Capture-MS Homo sapiens
40 DIABLO 56616
Affinity Capture-MS Homo sapiens
41 GPA33 10223
Affinity Capture-MS Homo sapiens
42 PLOD2 5352
Co-fractionation Homo sapiens
43 C12orf49  
Positive Genetic Homo sapiens
44 TAGLN2 8407
Co-fractionation Homo sapiens
45 ALDOC 230
Co-fractionation Homo sapiens
46 KIF23 9493
Affinity Capture-MS Homo sapiens
47 GLRX2  
Co-fractionation Homo sapiens
48 CACFD1  
Affinity Capture-MS Homo sapiens
49 CYB561  
Affinity Capture-MS Homo sapiens
50 ENO2 2026
Co-fractionation Homo sapiens
51 ENO1 2023
Co-fractionation Homo sapiens
52 PRDX6 9588
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
53 KRAS 3845
Negative Genetic Homo sapiens
54 PTPN11 5781
Co-fractionation Homo sapiens
55 CST6  
Affinity Capture-MS Homo sapiens
56 VDAC1 7416
Co-fractionation Homo sapiens
57 ETFA 2108
Co-fractionation Homo sapiens
58 DDX58 23586
Affinity Capture-RNA Homo sapiens
59 PRDX2 7001
Co-fractionation Homo sapiens
60 HINT1 3094
Co-fractionation Homo sapiens
61 FKBP2 2286
Co-fractionation Homo sapiens
62 VDAC2 7417
Co-fractionation Homo sapiens
63 COIL  
Proximity Label-MS Homo sapiens
64 ALDOA 226
Co-fractionation Homo sapiens
65 PPIF 10105
Co-fractionation Homo sapiens
66 ME1 4199
Co-fractionation Homo sapiens
View the network image/svg+xml



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