Gene description for PPIF
Gene name peptidylprolyl isomerase F
Gene symbol PPIF
Other names/aliases CYP3
CyP-M
Cyp-D
CypD
Species Homo sapiens
 Database cross references - PPIF
ExoCarta ExoCarta_10105
Entrez Gene 10105
HGNC 9259
MIM 604486
UniProt P30405  
 PPIF identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PPIF
Molecular Function
    cyclosporin A binding GO:0016018 IEA
    protein binding GO:0005515 IPI
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
Biological Process
    cellular response to calcium ion GO:0071277 ISS
    response to ischemia GO:0002931 ISS
    negative regulation of ATPase activity GO:0032780 ISS
    negative regulation of oxidative phosphorylation uncoupler activity GO:2000276 ISS
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IDA
    regulation of proton-transporting ATPase activity, rotational mechanism GO:0010849 ISS
    protein peptidyl-prolyl isomerization GO:0000413 IBA
    regulation of mitochondrial membrane permeability GO:0046902 ISS
    necroptotic process GO:0070266 IEA
    cellular response to arsenic-containing substance GO:0071243 ISS
    positive regulation of release of cytochrome c from mitochondria GO:0090200 ISS
    negative regulation of oxidative phosphorylation GO:0090324 ISS
    regulation of mitochondrial membrane permeability involved in programmed necrotic cell death GO:1902445 IMP
    apoptotic mitochondrial changes GO:0008637 IBA
    cellular response to hydrogen peroxide GO:0070301 IMP
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
    protein folding GO:0006457 IEA
    regulation of necrotic cell death GO:0010939 IEA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    membrane GO:0016020 TAS
    mitochondrial matrix GO:0005759 IBA
    mitochondrial proton-transporting ATP synthase complex GO:0005753 ISS
 Experiment description of studies that identified PPIF in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PPIF
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PPIF is involved
No pathways found





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