Gene description for PPIF
Gene name peptidylprolyl isomerase F
Gene symbol PPIF
Other names/aliases CYP3
CyP-M
Cyp-D
CypD
Species Homo sapiens
 Database cross references - PPIF
ExoCarta ExoCarta_10105
Vesiclepedia VP_10105
Entrez Gene 10105
HGNC 9259
MIM 604486
UniProt P30405  
 PPIF identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PPIF
Molecular Function
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IBA
    peptidyl-prolyl cis-trans isomerase activity GO:0003755 IDA
    protein binding GO:0005515 IPI
    cyclosporin A binding GO:0016018 IBA
    peptide binding GO:0042277 IEA
    RNA polymerase II CTD heptapeptide repeat P3 isomerase activity GO:0140839 IEA
    RNA polymerase II CTD heptapeptide repeat P6 isomerase activity GO:0140840 IEA
Biological Process
    response to ischemia GO:0002931 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein folding GO:0006457 IBA
    apoptotic mitochondrial changes GO:0008637 IEA
    regulation of proton-transporting ATPase activity, rotational mechanism GO:0010849 ISS
    negative regulation of ATP-dependent activity GO:0032780 ISS
    negative regulation of apoptotic process GO:0043066 IDA
    regulation of mitochondrial membrane permeability GO:0046902 IMP
    regulation of mitochondrial membrane permeability GO:0046902 ISS
    mitochondrial depolarization GO:0051882 IEA
    necroptotic process GO:0070266 IEA
    cellular response to hydrogen peroxide GO:0070301 IBA
    cellular response to hydrogen peroxide GO:0070301 IMP
    cellular response to arsenic-containing substance GO:0071243 ISS
    cellular response to calcium ion GO:0071277 ISS
    negative regulation of release of cytochrome c from mitochondria GO:0090201 IDA
    negative regulation of oxidative phosphorylation GO:0090324 ISS
    skeletal muscle fiber differentiation GO:0098528 IEA
    regulation of mitochondrial membrane permeability involved in programmed necrotic cell death GO:1902445 IBA
    regulation of mitochondrial membrane permeability involved in programmed necrotic cell death GO:1902445 IMP
    mitochondrial outer membrane permeabilization involved in programmed cell death GO:1902686 IMP
    negative regulation of oxidative phosphorylation uncoupler activity GO:2000276 ISS
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial permeability transition pore complex GO:0005757 IDA
    mitochondrial matrix GO:0005759 IEA
    membrane GO:0016020 TAS
 Experiment description of studies that identified PPIF in exosomes
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PPIF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 ACOT7 11332
Affinity Capture-MS Homo sapiens
3 SLIRP 81892
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
4 CLIP1 6249
Affinity Capture-MS Homo sapiens
5 SRPRB 58477
Co-fractionation Homo sapiens
6 TRUB2  
Proximity Label-MS Homo sapiens
7 MTG2  
Proximity Label-MS Homo sapiens
8 LGALS1 3956
Affinity Capture-MS Homo sapiens
9 NABP2  
Co-fractionation Homo sapiens
10 RPUSD4 84881
Proximity Label-MS Homo sapiens
11 MTRF1  
Proximity Label-MS Homo sapiens
12 GFM2 84340
Proximity Label-MS Homo sapiens
13 CCDC90B  
Proximity Label-MS Homo sapiens
14 LRPPRC 10128
Proximity Label-MS Homo sapiens
15 Ophn1  
Affinity Capture-MS Mus musculus
16 CRYZ 1429
Proximity Label-MS Homo sapiens
17 TFAM 7019
Proximity Label-MS Homo sapiens
18 PMPCA 23203
Proximity Label-MS Homo sapiens
19 TBL1XR1 79718
Co-fractionation Homo sapiens
20 HINT2 84681
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
21 MUC1 4582
Two-hybrid Homo sapiens
22 STX7 8417
Co-fractionation Homo sapiens
23 ETFA 2108
Co-fractionation Homo sapiens
24 CIDEB  
Two-hybrid Homo sapiens
25 RER1 11079
Affinity Capture-MS Homo sapiens
26 MRPS26 64949
Proximity Label-MS Homo sapiens
27 SURF1  
Proximity Label-MS Homo sapiens
28 EXD2  
Proximity Label-MS Homo sapiens
29 MOB1B 92597
Co-fractionation Homo sapiens
30 PMPCB 9512
Proximity Label-MS Homo sapiens
31 TACO1  
Proximity Label-MS Homo sapiens
32 FASTKD2  
Proximity Label-MS Homo sapiens
33 VWA8 23078
Proximity Label-MS Homo sapiens
34 SOD1 6647
Co-fractionation Homo sapiens
35 MTERF3  
Proximity Label-MS Homo sapiens
36 MDH2 4191
Proximity Label-MS Homo sapiens
37 E2F4  
Affinity Capture-MS Homo sapiens
38 BANP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 ANKRD20A8P  
Cross-Linking-MS (XL-MS) Homo sapiens
40 C6orf203  
Proximity Label-MS Homo sapiens
41 UBE4B 10277
Co-fractionation Homo sapiens
42 MAGEA11  
Two-hybrid Homo sapiens
43 FASTKD5  
Proximity Label-MS Homo sapiens
44 TUFM 7284
Proximity Label-MS Homo sapiens
45 ATF2  
Affinity Capture-MS Homo sapiens
46 AGTRAP 57085
Two-hybrid Homo sapiens
47 NELFE 7936
Affinity Capture-MS Homo sapiens
48 YIF1A 10897
Two-hybrid Homo sapiens
49 MYH9 4627
Affinity Capture-MS Homo sapiens
50 CCDC109B 55013
Proximity Label-MS Homo sapiens
51 GFM1 85476
Proximity Label-MS Homo sapiens
52 BCL6  
Affinity Capture-MS Homo sapiens
53 SLC25A4 291
Reconstituted Complex Homo sapiens
54 MRPL11 65003
Proximity Label-MS Homo sapiens
55 MTRF1L  
Proximity Label-MS Homo sapiens
56 TMEM70  
Proximity Label-MS Homo sapiens
57 ATP6V1E1 529
Co-fractionation Homo sapiens
58 COX4I1 1327
Proximity Label-MS Homo sapiens
59 SUGP1  
Co-fractionation Homo sapiens
60 ANLN 54443
Affinity Capture-MS Homo sapiens
61 TPX2  
Co-fractionation Homo sapiens
62 LIMA1 51474
Cross-Linking-MS (XL-MS) Homo sapiens
63 MRPL14 64928
Co-fractionation Homo sapiens
64 PTPN11 5781
Co-fractionation Homo sapiens
65 OTC 5009
Proximity Label-MS Homo sapiens
66 MRRF  
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
67 AIFM1 9131
Proximity Label-MS Homo sapiens
68 MRPS12  
Proximity Label-MS Homo sapiens
69 MTIF2 4528
Proximity Label-MS Homo sapiens
70 AIFM3  
Co-fractionation Homo sapiens
71 OTUD1 220213
Affinity Capture-MS Homo sapiens
72 CHCHD1  
Proximity Label-MS Homo sapiens
73 NME1-NME2 654364
Co-fractionation Homo sapiens
74 CLPP 8192
Proximity Label-MS Homo sapiens
75 MTIF3  
Proximity Label-MS Homo sapiens
76 RMND1 55005
Proximity Label-MS Homo sapiens
77 RBMS1 5937
Co-fractionation Homo sapiens
78 FNTA 2339
Affinity Capture-MS Homo sapiens
79 ZC3H11A  
Co-fractionation Homo sapiens
80 SYP  
Two-hybrid Homo sapiens
81 C12orf65  
Proximity Label-MS Homo sapiens
82 FAM25A  
Two-hybrid Homo sapiens
83 FAM25G  
Two-hybrid Homo sapiens
84 CMTM5  
Two-hybrid Homo sapiens
85 TRIM13  
Affinity Capture-MS Homo sapiens
86 GSTK1 373156
Co-fractionation Homo sapiens
87 USP24 23358
Cross-Linking-MS (XL-MS) Homo sapiens
88 ACAD9 28976
Proximity Label-MS Homo sapiens
89 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
90 METTL15  
Proximity Label-MS Homo sapiens
91 MCUR1 63933
Proximity Label-MS Homo sapiens
92 HSPE1 3336
Co-fractionation Homo sapiens
93 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
94 VDAC3 7419
Co-fractionation Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 TSFM 10102
Proximity Label-MS Homo sapiens
97 AUH 549
Proximity Label-MS Homo sapiens
98 RRBP1 6238
Co-fractionation Homo sapiens
99 TANGO2  
Co-fractionation Homo sapiens
100 NDUFAF2  
Co-fractionation Homo sapiens
101 ICT1 3396
Proximity Label-MS Homo sapiens
102 MRPL53  
Co-fractionation Homo sapiens
103 GPX4 2879
Co-fractionation Homo sapiens
104 S100A10 6281
Co-fractionation Homo sapiens
105 MPLKIP  
Co-fractionation Homo sapiens
106 COX8A  
Proximity Label-MS Homo sapiens
107 FKBP1A 2280
Co-fractionation Homo sapiens
108 FAM25C  
Two-hybrid Homo sapiens
109 ARFIP2 23647
Two-hybrid Homo sapiens
110 SSBP1 6742
Proximity Label-MS Homo sapiens
111 FASTKD3  
Proximity Label-MS Homo sapiens
112 ABI2 10152
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
113 PRB3  
Two-hybrid Homo sapiens
114 TBRG4 9238
Proximity Label-MS Homo sapiens
115 AARS2  
Proximity Label-MS Homo sapiens
116 TEFM  
Proximity Label-MS Homo sapiens
117 RPUSD3  
Proximity Label-MS Homo sapiens
118 C1QBP 708
Proximity Label-MS Homo sapiens
119 METTL17  
Proximity Label-MS Homo sapiens
120 RANGAP1 5905
Co-fractionation Homo sapiens
121 TIMM44 10469
Co-fractionation Homo sapiens
122 ALDOA 226
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which PPIF is involved
No pathways found





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