Gene description for GSTK1
Gene name glutathione S-transferase kappa 1
Gene symbol GSTK1
Other names/aliases GST
GST 13-13
GST13
GST13-13
GSTK1-1
hGSTK1
Species Homo sapiens
 Database cross references - GSTK1
ExoCarta ExoCarta_373156
Entrez Gene 373156
HGNC 16906
MIM 602321
UniProt Q9Y2Q3  
 GSTK1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for GSTK1
Molecular Function
    protein binding GO:0005515 IPI
    protein disulfide oxidoreductase activity GO:0015035 IEA
    receptor binding GO:0005102 IPI
    glutathione peroxidase activity GO:0004602 IDA
    glutathione transferase activity GO:0004364 IDA
Biological Process
    epithelial cell differentiation GO:0030855 IEP
    oxidation-reduction process GO:0055114 IEA
    glutathione metabolic process GO:0006749 IEA
Subcellular Localization
    mitochondrial inner membrane GO:0005743 IEA
    peroxisome GO:0005777 IDA
    intracellular GO:0005622 IDA
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    mitochondrial matrix GO:0005759 IEA
 Experiment description of studies that identified GSTK1 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GSTK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MCM7 4176
Affinity Capture-MS Homo sapiens
2 RPN2 6185
Affinity Capture-MS Homo sapiens
3 HNRNPM 4670
Affinity Capture-MS Homo sapiens
4 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
5 HRNR 388697
Affinity Capture-MS Homo sapiens
6 EPPK1 83481
Affinity Capture-MS Homo sapiens
7 DNAAF5 54919
Affinity Capture-MS Homo sapiens
8 FANCI 55215
Affinity Capture-MS Homo sapiens
9 ATXN10 25814
Affinity Capture-MS Homo sapiens
10 CCAR2 57805
Affinity Capture-MS Homo sapiens
11 CYCS  
Affinity Capture-MS Homo sapiens
12 XPO5 57510
Affinity Capture-MS Homo sapiens
13 LRPPRC 10128
Affinity Capture-MS Homo sapiens
14 EFTUD2 9343
Affinity Capture-MS Homo sapiens
15 ZW10 9183
Affinity Capture-MS Homo sapiens
16 KIF1BP 26128
Affinity Capture-MS Homo sapiens
17 SLC3A2 6520
Affinity Capture-MS Homo sapiens
18 IPO4 79711
Affinity Capture-MS Homo sapiens
19 TSC22D1  
Affinity Capture-MS Homo sapiens
20 DCTN1 1639
Affinity Capture-MS Homo sapiens
21 MATR3 9782
Affinity Capture-MS Homo sapiens
22 RPN1 6184
Affinity Capture-MS Homo sapiens
23 HTR1B  
Affinity Capture-MS Homo sapiens
24 IARS 3376
Affinity Capture-MS Homo sapiens
25 DDOST 1650
Affinity Capture-MS Homo sapiens
26 SEC63  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GSTK1 is involved
No pathways found





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