Gene description for HRNR
Gene name hornerin
Gene symbol HRNR
Other names/aliases FLG3
S100A16
S100a18
Species Homo sapiens
 Database cross references - HRNR
ExoCarta ExoCarta_388697
Vesiclepedia VP_388697
Entrez Gene 388697
HGNC 20846
MIM 616293
UniProt Q86YZ3  
 HRNR identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells 36408942    
Plasma 19028452    
 Gene ontology annotations for HRNR
Molecular Function
    calcium ion binding GO:0005509 IEA
    transition metal ion binding GO:0046914 IEA
Biological Process
    keratinization GO:0031424 IEA
    cell envelope organization GO:0043163 IDA
    establishment of skin barrier GO:0061436 IBA
    establishment of skin barrier GO:0061436 IEP
Subcellular Localization
    cornified envelope GO:0001533 IBA
    cornified envelope GO:0001533 IDA
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IDA
    azurophil granule lumen GO:0035578 TAS
    keratohyalin granule GO:0036457 IBA
    keratohyalin granule GO:0036457 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HRNR in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 44
MISEV standards
✔
IEM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for HRNR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cep290  
Affinity Capture-MS Mus musculus
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 CEP57  
Affinity Capture-MS Homo sapiens
4 DIRAS2 54769
Affinity Capture-MS Homo sapiens
5 ANLN 54443
Affinity Capture-MS Homo sapiens
6 POT1  
Affinity Capture-MS Homo sapiens
7 FLG2 388698
Affinity Capture-MS Homo sapiens
8 MECP2 4204
Affinity Capture-MS Homo sapiens
9 AIMP2 7965
Affinity Capture-MS Homo sapiens
10 RRM2 6241
Affinity Capture-MS Homo sapiens
11 SERBP1 26135
Affinity Capture-MS Homo sapiens
12 PARP1 142
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
13 SOD1 6647
Affinity Capture-MS Homo sapiens
14 RLIM 51132
Affinity Capture-MS Homo sapiens
15 METTL14  
Affinity Capture-MS Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 ESR1  
Affinity Capture-MS Homo sapiens
18 EFNA1  
Affinity Capture-MS Homo sapiens
19 DDX3X 1654
Affinity Capture-MS Homo sapiens
20 BTF3 689
Affinity Capture-MS Homo sapiens
21 IQCB1  
Affinity Capture-MS Homo sapiens
22 LGALS3 3958
Affinity Capture-MS Homo sapiens
23 PDHA1 5160
Affinity Capture-MS Homo sapiens
24 DSG1 1828
Affinity Capture-MS Homo sapiens
25 JUP 3728
Affinity Capture-MS Homo sapiens
26 UBC 7316
Reconstituted Complex Homo sapiens
27 EGFR 1956
Affinity Capture-MS Homo sapiens
28 CSTA 1475
Affinity Capture-MS Homo sapiens
29 PINX1  
Affinity Capture-MS Homo sapiens
30 CDK2 1017
Affinity Capture-MS Homo sapiens
31 Ddb1 13194
Affinity Capture-MS Mus musculus
32 PARK7 11315
Affinity Capture-MS Homo sapiens
33 FABP5 2171
Affinity Capture-MS Homo sapiens
34 CDK1 983
Affinity Capture-MS Homo sapiens
35 SERPINB12 89777
Affinity Capture-MS Homo sapiens
36 CUL9  
Affinity Capture-MS Homo sapiens
37 PPP1R16B  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
38 AURKB 9212
Affinity Capture-MS Homo sapiens
39 EFNA3  
Affinity Capture-MS Homo sapiens
40 KHDRBS2  
Affinity Capture-MS Homo sapiens
41 COQ2  
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 TRIM37  
Proximity Label-MS Homo sapiens
44 SOAT1 6646
Affinity Capture-MS Homo sapiens
45 LINC00152  
Affinity Capture-RNA Homo sapiens
46 FBXO6 26270
Affinity Capture-MS Homo sapiens
47 MYC  
Affinity Capture-MS Homo sapiens
48 ZUFSP 221302
Affinity Capture-MS Homo sapiens
49 DYRK1A 1859
Affinity Capture-MS Homo sapiens
50 TRIM21 6737
Affinity Capture-MS Homo sapiens
51 KIF23 9493
Affinity Capture-MS Homo sapiens
52 MCM2 4171
Affinity Capture-MS Homo sapiens
53 CDKN3 1033
Affinity Capture-MS Homo sapiens
54 GSTK1 373156
Affinity Capture-MS Homo sapiens
55 FOXN3 1112
Affinity Capture-MS Homo sapiens
56 METTL3  
Affinity Capture-MS Homo sapiens
57 Invs  
Affinity Capture-MS Mus musculus
58 COPS5 10987
Affinity Capture-MS Homo sapiens
59 MYCN  
Affinity Capture-MS Homo sapiens
60 CCDC8  
Affinity Capture-MS Homo sapiens
61 TXNIP 10628
Affinity Capture-MS Homo sapiens
62 WTAP 9589
Affinity Capture-MS Homo sapiens
63 CHMP4B 128866
Affinity Capture-MS Homo sapiens
64 ARF1 375
Proximity Label-MS Homo sapiens
65 VDAC1 7416
Affinity Capture-MS Homo sapiens
66 BTK 695
Affinity Capture-MS Homo sapiens
67 STYX  
Affinity Capture-MS Homo sapiens
68 CUL2 8453
Affinity Capture-MS Homo sapiens
69 CEP76  
Affinity Capture-MS Homo sapiens
70 DDX39B 7919
Affinity Capture-MS Homo sapiens
71 PRC1 9055
Affinity Capture-MS Homo sapiens
72 KLF5  
Affinity Capture-MS Homo sapiens
73 MDM2  
Affinity Capture-MS Homo sapiens
74 CYLD  
Affinity Capture-MS Homo sapiens
75 CUL4A 8451
Affinity Capture-MS Homo sapiens
76 PPME1 51400
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
77 KPRP 448834
Affinity Capture-MS Homo sapiens
78 AR 367
Affinity Capture-MS Homo sapiens
79 DSP 1832
Affinity Capture-MS Homo sapiens
80 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HRNR is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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