Gene description for AIMP2
Gene name aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
Gene symbol AIMP2
Other names/aliases JTV-1
JTV1
P38
PRO0992
Species Homo sapiens
 Database cross references - AIMP2
ExoCarta ExoCarta_7965
Entrez Gene 7965
HGNC 20609
MIM 600859
UniProt Q13155  
 AIMP2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for AIMP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    Type II pneumocyte differentiation GO:0060510 IEA
    tRNA aminoacylation for protein translation GO:0006418 TAS
    apoptotic process GO:0006915 IEA
    negative regulation of cell proliferation GO:0008285 IEA
    positive regulation of protein ubiquitination GO:0031398 IEA
    gene expression GO:0010467 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified AIMP2 in exosomes
1
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AIMP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FHL3  
Two-hybrid Homo sapiens
2 LNX  
Two-hybrid Homo sapiens
3 KARS 3735
Two-hybrid Homo sapiens
4 EPS8 2059
Two-hybrid Homo sapiens
5 DARS 1615
Two-hybrid Homo sapiens
6 CEP170L  
Two-hybrid Homo sapiens
7 MGC11271  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
9 LMO3  
Two-hybrid Homo sapiens
10 AIMP2 7965
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 EPRS 2058
Invivo Homo sapiens
12 AIMP1 9255
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which AIMP2 is involved
PathwayEvidenceSource
Cytosolic tRNA aminoacylation TAS Reactome





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