Gene description for TANK
Gene name TRAF family member-associated NFKB activator
Gene symbol TANK
Other names/aliases I-TRAF
ITRAF
TRAF2
Species Homo sapiens
 Database cross references - TANK
ExoCarta ExoCarta_10010
Vesiclepedia VP_10010
Entrez Gene 10010
HGNC 11562
MIM 603893
UniProt Q92844  
 TANK identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TANK
Molecular Function
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
Biological Process
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    innate immune response GO:0045087 TAS
    I-kappaB kinase/NF-kappaB signaling GO:0007249 IEA
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    signal transduction GO:0007165 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified TANK in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TANK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TBK1 29110
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 MAP4K5 11183
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 IKBKG 8517
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 ITGB1BP1 9270
Affinity Capture-Western Homo sapiens
5 USP7 7874
Invitro Homo sapiens
6 TRAF2 7186
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 CHUK 1147
Affinity Capture-Western Homo sapiens
8 TRAF1 7185
Two-hybrid Homo sapiens
9 IKBKE 9641
Invivo Homo sapiens
Two-hybrid Homo sapiens
10 MCPIP  
Two-hybrid Homo sapiens
11 TRAF3 7187
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TANK is involved
PathwayEvidenceSource
Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon TAS Reactome
TRAF6 mediated IRF7 activation TAS Reactome





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